162,640 research outputs found
[Report to Chief J. E. Curry, by an unknown author #1]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
[Report to Chief J. E. Curry, by an unknown author #2]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
VAR, SVAR and SVEC Models: Implementation Within R Package vars
No full textThe structure of the package vars and its implementation of vector autoregressive, structural vector autoregressive and structural vector error correction models are explained in this paper. In addition to the three cornerstone functions VAR(), SVAR() and SVEC() for estimating such models, functions for diagnostic testing, estimation of a restricted models, prediction, causality analysis, impulse response analysis and forecast error variance decomposition are provided too. It is further possible to convert vector error correction models into their level VAR representation. The different methods and functions are elucidated by employing a macroeconomic data set for Canada. However, the focus in this writing is on the implementation part rather than the usage of the tools at hand.
MSC-derived exosomes inhibit proliferation and migration of SVEC cells <i>in vitro</i>.
No full text<p>(A) 2.0×10<sup>3</sup> SVEC cells were incubated with the conditioned media from 4T1 cells that were treated with various concentrations of MSC-derived exosomes or carrier control (PBS). Cell proliferation rates were determined by an EZ-Cytox cell viability assay kit. (B) SVEC cells transwell migration assay was performed in the presence of the conditioned media from 4T1 cells that were treated with various concentrations of MSC-derived exosomes or carrier control (PBS) in the lower chambers. Serum-starved SVEC cells were added to the upper chamber and incubated for 24 h to allow cell migration through the membrane. The membranes were stained with crystal violet and cell migration was analyzed by Image J. (C) SVEC cells were scratched and incubated with the conditioned media from 4T1 cells stimulated with MSC-derived exosomes (100 μg/ml) or vehicle control (PBS) for 24 h. In order to neutralize VEGF derived from 4T1 cells, anti-VEGF antibodies (20 μg/ml) were added to the conditioned media. Photographs were taken immediately and 24 h after wounding (data not shown) and analyzed by Studio Lite, version 1.0. (D) SVEC cells were serum-starved for 24 h and 2×10<sup>4</sup> SVEC cells were seeded in a Matrigel-coated well. The cells were treated with conditioned media collected from 4T1 cells stimulated with MSC-derived exosomes (100 μg/ml) or carrier control (PBS) for 24 h and viewed under a microscope. The values are presented as the mean ± SD; n = 3 for each group. Significant differences were evaluated using an unpaired two-tailed Student's t-test. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 compared with control.</p
Manipulated wettability of a superhydrophobic quartz crystal microbalance through electrowetting
No full textThe liquid phase response of quartz crystal microbalances (QCM) with a thin coating (~9 µm) of epoxy resin with and without a carbon nanoparticles top layer is reported. The nanoparticles convert the epoxy surface to a superhydrophobic one with a high static contact angle (~151º-155º) and low contact angle hysteresis (~1º-3.7º) where droplets of water are in the suspended Cassie-Baxter state. The frequency decrease of the fully immersed QCM with the superhydrophobic surface is less than with only epoxy layer, thus indicating a decoupling of the QCM response. A wettability transition to a liquid penetrating into the surface roughness state (for droplets a high contact angle hysteresis Wenzel state) was triggered using a molarity of ethanol droplet test (MED) and electrowetting; the MED approach caused some surface damage. The electrowetting induced transition caused a frequency decrease of 739 Hz at a critical voltage of ~100 V compared to the QCM in air. This critical voltage correlates to a contact angle decrease of 26º and a high contact angle hysteresis state in droplet experiments. These experiments provide a proof-of-concept that QCMs can be used to sense wetting state transitions and not only mass attachments or changes in viscosity-density products of liquids
Development of imidazotetrazines for the treatment of glioblastoma & as synthetic precursors to diazo species
No full textGlioblastoma multiforme (GBM), a grade IV astrocytoma, is the most prevalent, aggressive, and deadly form of malignant brain tumor with only 10% of patients surviving five years. The current standard treatment for GBM patients is bulk surgical resection and radiotherapy with concomitant and adjuvant temozolomide (TMZ), a small molecule DNA alkylating agent. The antitumor effect of TMZ is ultimately mediated through methylation of the O6-position of guanine and subsequent mismatch repair (MMR)-dependent cell death. Although TMZ was first synthesized in 1984 and has been FDA approved for two decades, certain key derivatives have been inaccessible due to synthetic challenges, precluding a broad exploration of the link between imidazotetrazine structure and biological activity. Novel synthetic strategies provided access to several new classes of C8-substituted imidazotetrazines and an evaluation of their aqueous stabilities enabled the derivation of a predictive model for imidazotetrazine hydrolytic stability based on the Hammett constant of the C8 substituent. Promising compounds modified at the C8 position were identified and found to have appropriate hydrolytic stability, enhanced blood-brain barrier permeability, lower hematological toxicity profiles, and superior activity relative to TMZ in a mouse model of GBM.
Despite its widespread use, certain GBM patient populations do not respond to TMZ therapy. Expression of O6-methylguanine DNA methyltransferase (MGMT) and loss of MMR function are the primary clinical modes of resistance to TMZ. Imidazotetrazine analogs modified at the N3 position were designed to deliver alternative DNA adducts that were irremovable by MGMT. An analog bearing a propargyl group at N3 demonstrated MGMT-independent activity in GBM cells in culture. Tuning the stability with an electron-donating chloro substituent at the C8 position produced novel dual-substituted imidazotetrazine CPZ, which displayed anticancer activity irrespective of MGMT expression and MMR status.
Imidazotetrazines such as TMZ are precursors to alkyl diazoniums, used therapeutically to alkylate DNA and elicit an anticancer effect. Given their stability as prodrugs and the reactivity of the species released, imidazotetrazines were repurposed into synthetic surrogates for diazomethane and other diazoalkanes. TMZ was employed to conduct esterifications and metal-catalyzed cyclopropanations, with methyl ester formation from a wide variety of substrates proving efficient and operationally simple. As a commercially available solid that is non-explosive and non-toxic, TMZ should find broad utility as a synthetic replacement for diazomethane.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2022-05-01The student, Riley Svec, accepted the attached license on 2020-04-14 at 17:59.The student, Riley Svec, submitted this Dissertation for approval on 2020-04-14 at 18:07.This Dissertation was approved for publication on 2020-04-16 at 13:46.DSpace SAF Submission Ingestion Package generated from Vireo submission #14980 on 2020-08-25 at 17:40:15Made available in DSpace on 2020-08-27T00:49:56Z (GMT). No. of bitstreams: 3
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Previous issue date: 2020-04-16Embargo set by: Seth Robbins for item 115864
Lift date: 2022-08-27T00:50:22Z
Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 115864
Lift date: 2022-08-27T00:51:40Z
Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemAuthor requested closed access (OA after 2yrs) in Vireo ETD systemLimite
Murder on the mountain: author talk with Peter J. Wosh
No full textAuthor talk by Peter J. Wosh on May 5th, 2022, on his book, "Murder on the Mountain: crime, passion, and punishment in gilded age New Jersey.
Mr. Melvin J. Collier, RWWL AUC, June 2011
No full textThis video is a conversation with Mr. Melvin J. Collier. Mr. Collier talks about his book, "From Mississippi to Africa: A Journey of Discovery". Daniel Le, AUC Woodruff Library, is the interviewer
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