7 research outputs found
Production and evaluation of high specific activity 186Re: An isotope for radioimmunotherapy in cancer treatment
No full textCyclotron production of high–specific activity 55Co and in vivo evaluation of the stability of 55Co metal-chelate-peptide complexes
No full textThis work describes the production of high–specific activity 55 Co and the evaluation of the stability of 55 Co-metal-chelate-peptide complexes in vivo. 55 Co was produced via the 58 Ni(p,α) 55 Co reaction and purified using anion exchange chromatography with an average recovery of 92% and an average specific activity of 1.96 GBq/μmol. 55 Co-DO3A and 55 Co-NO2A peptide complexes were radiolabeled at 3.7 MBq/μg and injected into HCT-116 tumor xenografted mice. Positron emission tomography (PET) and biodistribution studies were performed at 24 and 48 hours postinjection and compared to those of 55 CoCl 2 . Both 55 Co-metal-chelate complexes demonstrated good in vivo stability by reducing the radiotracers’ uptake in the liver by sixfold at 24 hours with ˜ 1% ID/g and at 48 hours with ˜ 0.5% ID/g and reducing uptake in the heart by fourfold at 24 hours with ˜ 0.7% ID/g and sevenfold at 48 hours with ˜ 0.35% ID/g. These results support the use of 55 Co as a promising new radiotracer for PET imaging of cancer and other diseases
Empowering Ni-Vanuatu women: Amplifying Wantok authority and achieving fair market access
No full textThe Republic of Vanuatu (2004) report on Vanuatu’s implementation of the United Nations Convention on the Elimination of All Forms of Discrimination against Women (CEDAW) notes that many urban disenfranchised Ni-Vanuatu women live in poverty and have little access to paid employment. The women who do gain paid employment in formal jobs rarely gain access to positions of authority. The United Nations (UN) offered two strategies to improve the position of Ni-Vanuatu women in Vanuatu. The first is informed by CEDAW in Article Eleven on Employment. The “Equity Desk of the Vanuatu Department of Strategic Management” and the “Vanuatu Department of Women’s Affairs Gender Planner” (The Republic of Vanuatu, 2004, pp. 12-13) have been charged with the responsibility of implementing Article Eleven and developing Equal Employment Opportunities (EEO) programmes for the public sector. This strategy aims to increase women’s access to paid employment in the formal employment sector and encourage women to achieve positions of authority. The second strategy offered by the UN is the establishment of microfinance projects aimed at providing disenfranchised urban women unable to find employment with a means to own and run microfinance businesses to earn a living. Both these strategies have the overarching aim of improving the well-being of Ni-Vanuatu women.
This study has investigated the extent to which access to formal sector jobs and the implementation of microfinance businesses in the informal sector addresses the well-being of Ni-Vanuatu women. These programmes are being implemented within a complex historical, socio-political cultural and economic environment (Van Trease, 1995). This complexity includes the continuance of Wantok systems of governance in the form of matrilineality (predominant in Vanuatu) and patrilineality (adopted from Christian influences in 1800s and colonial legacy in 1906) (Van Trease, 1987; Facey, 1981; Allen, 1981 & Macdonald-Milne & Thomas, 1981). Matrilineal cultural values bequeath patrimony and legacy of lineage and land inheritance from mothers to daughters. Matrilineal women share power with men in community affairs (Maltali, Sandy & Tamashiro, 2009). In patrilineal communities, patrimony and legacy of lineage and land inheritance is passed from fathers to sons (Van Trease, 1987). Patrilineal mothers and daughters have no lineage, land inheritance, or power-sharing rights (Stege, Maetala, Naupa & Simo 2008). Both Wantok systems are based on communal values practised primarily in the rural sector. Urban centres are organised around a modern-cash and market-economy and a governance framework based on the British Westminster model and the French Head of State model (ILO, 2006). This European generated governance system is underpinned by values informed by liberal competitive individualism and an assumed commitment to meritocracy. It is, however, a system of governance steeped in patriarchal nuances as a direct legacy of the colonial regime now adapted and administered by the Vanuatu’s ruling elite, referred as Vanuatu’s urban patriarchy throughout this thesis.
The theoretical frameworks used in this research draw on both liberal feminist studies and on an adaptation of subaltern scholarship (Thomas & Humphries, 2010 & 2011). The focus is on the legacy of imperialism and colonisation, the politics of power and hegemony, and the expressions of equal rights, emancipation and empowerment as these pertain to the well-being of women in Vanuatu.
Three sets of qualitative empirical observations were collected: i) a focus group discussion with 20 employer and employee representatives; ii) 36 conversations with women employed in the formal employment sector who held positions of authority within their respective organisations; and iii) 39 conversations with women who owned a microfinance business. My field notes were analysed thematically using a point and counterpoint framework crafted from my interest in the work of Huxley (cf Baker & James, 2000a & 2000b & Dawson, 2009). The point is informed by a liberal feminist lens (Gamble, 1999 & Heywood, 2000). A counterpoint to this liberal feminist interpretation is generated from a post-colonial feminist perspective through an adaptation of subaltern studies (Thomas & Humphries 2010 & 2011; Gamble, 1999 & Spivak, 1988). I draw on my Matrilineal Wantok Feminist Voice (MWFV) to form a standpoint in the discussion and to frame insights drawn from the ideas associated with the solidarity economy (Allard, Davidson & Matthaei, 2009; Harvey, 2006 & Harding, 2004).
Point/counterpoint/standpoint for the research as a whole
Point: Liberal feminist strategies for the emancipation of women (and the intended improvement and well-being of their families associated with this perspective) encourage women to pursue better living standards, achieve empowerment in the home, and seek formal jobs or other market-based income opportunities. If in formal jobs, women are encouraged to seek positions of authority. For these women, the major transition in orientation is the move from Wantok-related patterns of responsibilities and opportunities to those made available in the formal Western-generated economy. These Western ways, with emphasis on individualized opportunity, appear to offer financial gain and familial influences, particularly to women born into patrilineal lineage descent groups.
Counterpoint: Viewed through the adaptation of subaltern perspectives that I have applied to the liberal feminist remedies for the enhancement of well-being for the women of Vanuatu, it appears that the women of Vanuatu are involved in multiple and simultaneous complex master/slave relationships (Kohn, 2005 & Honderich, 1995). These relationships are exemplified in salaried/professional occupations held by women, between the women and their employers and work-place cultures, between women and rural and urban patriarchal hegemonies, and between women and the cash and market economy. While EEO activities can be seen to make a difference in the lives of some women, taken together, these interventions are reducing the overall well-being for Ni-Vanuatu women more generally. For the Vanwods microfinance women entrepreneurs, master/slave relationships could be discerned between the Vanwods MFI’s social control of the Mamas, the Vanuatu Government’s imposition of high business licence fees to the Mamas, the Mamas and their greater dependence on the cash and market economy, and the Mamas and their relationship with rural and urban patriarchal hegemonies (Thomas & Humphries, 2010 & 2011). These forms of systemic subservience interpreted from the women’s narratives provide a caution against the uncritical adoption of Western liberal feminist ideals (DeVault, 1990). It is matrilineal women; however, who appear to suffer the most from their move into the urban centres as there they must contend with an urban patriarchal hegemony, an impediment which they had not encountered in their former rural communities governed in accordance with matrilineal Wantok values.
Standpoint: The research findings suggest that all women in this study worked long hours, experiencing discrimination and oppression, received low pay, and experienced increased financial obligations as a result of their engagement in formal and informal jobs. As well as being increasingly dependent on inadequate and unsustainable livelihoods in the urban areas, family and Wantok social relations were challenged and diminished as a consequence of their necessary commitment to their jobs and the demands of urban living. Access to traditional forms of authority and sustenance was undermined.
I conclude that, overall, the implementation of CEDAW-EEO programmes along with the establishment of microfinance projects devised for the emancipation of the disenfranchised women of Vanuatu, while apparently proving beneficial from a liberal feminist interpretation in granting urban women with access to incomes, property and power-sharing, may provide an element of liberation for women of patrilineal descent groups but add new dimensions of patriarchal inhibitors for women of matrilineal descent groups who take up employment under the Westminster rules of governance. The remedies taken as a whole, while promising improved well-being through market-based income generation, remove women from the Wantok kinship social support networks embedded in their indigenous Wantok governance frameworks causing complex problems and hardships for them. Drawing on my Matrilineal Wantok Feminist standpoint position, I suggest that the Solidarity Economy, which combines aspects of market access while still engaging in the traditional systems of social organization, offers an alternative organisational and economic framework for developing and enhancing community well-being in both the rural and urban areas of Vanuatu
Harvesting Cu-67 from the Collection of a Secondary Beam Cocktail at the National Superconducting Cyclotron Laboratory
No full textIsotope harvesting is a promising new method to obtain isotopes for which there is no reliable continuous supply at present. To determine the possibility of obtaining radiochemically pure radioisotopes from an aqueous beam dump at a heavy-ion fragmentation facility, preliminary experiments were performed to chemically extract a copper isotope from a large mixture of projectile fragmentation products in an aqueous medium. In this work a 93 MeV/u secondary beam cocktail was collected in an aqueous beam stop at the National Superconducting Cyclotron Laboratory (NSCL) located on the Michigan State University (MSU) campus. The beam cocktail consisted of similar to 2.9% Cu-67 in a large mixture of co-produced isotopes ranging in atomic number from similar to 19 to 34. The chemical extraction of 67Cu was achieved via a two-step process: primary extraction using a divalent metal chelation disk followed by anion-exchange chromatography. A significant fraction (74 +/- 4%) of the 67Cu collected in the aqueous beam stop was recovered with \u3e99% radiochemical purity. To illustrate the utility of this product, the purified 67Cu material was then used to radiolabel an anti-EGFR antibody, Panitumumab, and injected into mice bearing colon cancer xenografts. The tumor uptake at 5 days postinjection was found to be 12.5 +/- 0.7% which was in very good agreement with previously reported studies with this radiolabeled antibody. The present results demonstrate that harvesting isotopes from a heavy-ion fragmentation facility could be a promising new method for obtaining high-quality isotopes that are not currently available by traditional methods
A diachronic study into the distributions of two Italo-Romance synthetic conditional forms
No full textTwo distinct conditional paradigms are available to speakers of Italian, derived from the Latin periphrases cantare habui/cantare habebam. The aim of this thesis is to describe and explain their patterns of attestation in the earliest northern Italian and Tuscan texts, which date from between 1200 and 1400.
Textual analysis showed that while the cantare habui periphrasis was native to both areas, the use of the cantare habebam periphrasis differed in the northern and central dialects. In the northern dialects, the cantare habebam periphrasis was attested in all genres over the whole time period, whereas in the Tuscan dialects it only appeared in literary genres. Moreover, although the northern texts attested both periphrases consistently over time in every genre, only Tuscan poetry followed this pattern. Other genres attested reflexes of the cantare habebam periphrasis for short periods in the fourteenth century. These results suggest that different influences resulted in different patterns of conditional use in the two areas.
This thesis postulates that in the northern Italo-Romance dialects the cantare habebam periphrasis was introduced through the proximity to, and influence of, Provençal. Although the use of reflexes of cantare habebam was reinforced in the north by the Sicilian school of poets, the dual nature of the sources meant that it was also retained in prose, and thence into modern dialect use. In contrast, reflexes of the cantare habebam periphrasis were introduced into central Italy through the Sicilian school alone. Although it appeared in prose texts, this was a sporadic phenomenon, resulting from imitation of the influential poetic texts. Because there was no prose source for reflexes of the cantare habebam periphrasis, it did not enter non-literary genres and quickly disappeared from literary prose genres. The cantare habebam periphrasis eventually disappeared entirely from Tuscan poetry as well, and is not attested at all in the modern central dialects
Sustained biochemical response to oral antibiotics in pediatric PSC and ASC is correlated to changes in gut microbiota and serum bile acids during therapy
No full textObjectives and Study: Concomitant presence of autoimmune hepatitis and primary sclerosing cholangitis (PSC) is labelled as autoimmune sclerosing cholangitis (ASC) in children. Based upon the possible implication of microbiota and microbial metabolites, such as bile acids (BAs), in the pathogenesis of PSC, oral antibiotics are increasingly being used as a novel therapeutic approach and shown to have benefit in PSC but their role in pediatric ASC is not well evaluated. We prospectively analysed the gut microflora and serum BAs pool before and after antibiotic therapy in children with ASC or PSC alone, and evaluated whether changes in gut microflora and/or BAs correlated with response to treatment. Method: Patients diagnosed with ASC or PSC on basis of biochemical, liver biopsy and radiology findings were included. They prospectively received metronidazole (MTZ) for 14 days as induction or rescue therapy. MTZ were administrated in addition to the standard treatment of UDCA for PSC patients and azathioprine, and UDCA and/or steroids for ASC patients. Stool and serum samples were collected before and after MTZ therapy. DNA isolation, amplification and Illumina sequencing to profile the microbiota composition were performed using the bacterial 16s rRNA while serum BAs were assessed by ultraperformance liquid chromatography coupled to mass spectrometry. The beta-diversity measured the dissimilarity between each paired stool samples. The outcome parameters to assess the efficacy of antibiotics were reduction liver enzymes and subsequently achievement of sustained biochemical remission. Results: Seven children (4 ASC, 3 PSC) were included, of which 5 have a concomitant ulcerative colitis (UC). All patients showed a significant decrease in their AST (-55%, p< 0.025), ALT (-56%, p< 0.025) and GGT (-41%, p< 0.025) under MTZ. Three children relapsed after stopping MTZ while the four others children showed a sustained biochemical remission (liver enzymes below 1.5 times upper limit of normal) after a median follow-up of 375 days. Among these four patients, three exhibited a wide different microbial composition before and after MTZ as expressed by the beta-diversity variation. They also expanded their total serum BAs size (from 1542.96 to 2620.99 pmol/100μl, +69.87%), primarily due to the large increment of UDCA and its glycine- and taurine-conjugates which finally represented more than half of the total serum BAs. On the contrary, the microbiota of patients who relapsed remained unchanged pre- and post-MTZ and total BAs pool decreased. Conclusion: Our study suggests that oral antibiotic could be an effective treatment of ASC and PSC, especially those with a concomitant UC, and that intestinal microflora and BAs play a major role in these diseases as sustained biochemical remission is associated with wide changes in gut microbiota communities and BAs profile after taking antibiotics
