312 research outputs found
Corrigendum to “La-doped Sr4Fe4Co2O13-δ as a promising in-situ self-assembled composite cathode for protonic ceramic fuel cells” [Compos. B Eng. 280 (2024) 111517]
No full textThe authors regret that the email address of another corresponding author Dr. Shanshan Jiang is not specified. Her email address is [email protected] . The authors would like to apologise for any inconvenience caused
Regulation of organic anion transporters: molecular and cellular mechanisms
No full textHuman organic anion transporters (hOATs) play critical roles in the body disposition of clinically important drugs. Understanding the regulation of hOATs has profound clinical significance. My thesis work focuses on delineating molecular and cellular mechanisms underlying hOAT regulation, and consists of three parts. In the first part, we examined the regulation of hOAT trafficking and function by ubiquitination through cell signaling pathways (protein kinase C, protein kinase A, and their upstream hormones AngII and bradykinin) by combined approaches of cellular biology, site-directed mutagenesis and mass spectroscopy. We identified PKC isoforms involved in OAT regulation. We also identified lysine residues serving as ubiquitin-conjugating sites. In the second part, we examined transporter-mediated drug-drug interactions by screening two prescription drug libraries against hOAT1 and hOAT3. High potent inhibitors were identified. Computational analyses reveal several important properties which differentiate between inhibitors and non-inhibitors. Such model provides mechanistic insights for predicting new OAT inhibitors. In the third part, we studied structure and function relationship of hOATs. Critical transmembrane domain and amino acid residues were identified, which play important role in OAT stability, maturation efficiency as well as ologomerization.Ph. D.Includes bibliographical referencesIncludes vitaby Shanshan L
Enhanced physicochemical and functional properties of pea (Pisum sativum) protein by pH-shifting and ultrasonication combined process
No full textIn recent years, pea protein as a novel food ingredient has drawn increasing attention due to its high nutritional value, hypoallergenic, and low price. As an amphiphilic molecule, protein is known as a natural and bio-safe emulsifier. However, similar to other legume proteins, the low water solubility and poor functional properties of pea protein limit its applications in the food industry. This study was undertaken to investigate the effects of pH-shifting in combination with ultrasonication on the structural and physicochemical properties of pea protein isolate (PPI).
PPI dispersions (30 mg/ml each) were treated with ultrasonication, pH-shifting, and pH-shifting in combination with ultrasound and compared to control (no treatment). Water solubility, particle size, solution turbidity, surface hydrophobicity, free sulfhydryl group content, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the soluble pea protein obtained by the above treatments were determined. The PPI samples (10 mg/ml) treated with pH-shifting at pH 12 in combination with ultrasound (pH12+U5), which had highest solubility, were used to prepare nanoemulsions (0.25% oil) and nanocomplexes loaded with vitamin D3 (VD3). Storage stability, photooxidation protective ability, and morphological structure of the PPI-stabilized nano-systems were examined.
The pH12+U5 treatment increased the solubility of PPI from 8.17% (Control) to 60.83%, and reduced the volume-weighted mean diameters D [4, 3] of the soluble protein aggregates from 206.9 (Control) to 45.2 nm. The surface hydrophobicity of the pH12+U5-treated PPI was significantly higher than that of the native protein, while its free sulfhydryl group content was slightly decreased. Structural rearrangement of the treated PPI was observed in the SDS-PAGE, showing that the alkaline pH-shifting and ultrasonic treatment can disrupt covalent and non-covalent bonds. Even though there was no significant improvement in the antioxidant activity of the pH12+U5-processed protein compared to the native PPI, it exhibited good radical scavenging ability. After exposure to UV-light (312 nm, 15 W) for 180 minutes, the VD3 retained in the PPI-based nanoemulsion and nanocomplex was 74.22% and 65.37%, respectively, in contrast to 8.71% in the Control, demonstrating a good photooxidation protection ability of the nano-structures. Besides, the D [4, 3] of the droplets in the nanoemulsion and nanocomplex stabilized by the pH12+U5-treated PPI were 113.93 and 88.90 nm, respectively, and both nano-systems exhibited good stability during storage for 30 days.
In summary, the combination of pH-shifting and ultrasonication effectively improved the structural and physicochemical properties of pea protein isolate. The pea protein isolate processed with this new method would be a promising carrier to deliver and protect lipophilic bioactive components in food products, which could lead to foods with improved flavor, nutritional value, and shelf life.Submission published under a 24 month embargo labeled 'U of I only', the embargo will last until 2017-08-01The student, Shanshan Jiang, accepted the attached license on 2015-07-22 at 21:45.The student, Shanshan Jiang, submitted this Thesis for approval on 2015-07-22 at 22:01.This Thesis was approved for publication on 2015-07-24 at 12:01.DSpace SAF Submission Ingestion Package generated from Vireo submission #8620 on 2015-09-29 at 15:00:57Made available in DSpace on 2015-09-29T20:50:24Z (GMT). No. of bitstreams: 2
JIANG-THESIS-2015.pdf: 2037667 bytes, checksum: ed7d3df4d9efc82a05ddc72e1d73e2b5 (MD5)
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Previous issue date: 2015-07-24Embargo set by: Seth Robbins for item 89509
Lift date: 2017-09-29T20:50:34Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemU of I Only Restriction Lifted for Item 89509 on 2017-09-30T09:15:22Z
Split-step orthogonal spline collocation method for the complex Ginzburg-Landau equation in two dimensions
No full textThe computer coordination method and research of inland river traffic based on ship database
No full textRethinking the everydayness of Dutch 20th-Century malls
No full textArchitecture, Urbanism and Building Sciences | Heritage & Architectur
Inhibitory effects of crude extracts from several plants on postharvest pathogens of citrus
No full textBuilding Biographies through Heritage Repurposing: Socio-spatial Research in Shipai Village under Rapid Urbanization
No full textChinese urban village has attracted much discussion as an urban phenomenon by sociologists and architects. It was transformed from a traditional village gathered by clans with blood ties to an urban enclave focusing on a land-profit economy. At the same time, with the migration of locals and outsiders, the collective memory has changed from a mono-cultural memory to a multicultural memory. And problems such as poor infrastructure, unspoken rules of space usage and social segregation in the urban village have become increasingly severe during urbanization. However, there is not enough research on the urban village to explore the causes of such social conflicts based on the spatial conflicts. This article focuses on the Shipai village in Guangzhou and examed Lefebvre's theory of spatial production to explore the relationships between the changes in collective memory and spatial conflicts in Shipaii village. Based on the perspective of urban ethnography, the repurposing of heritage, such as the transformation of ancestral halls and streets, will be used as spatial outcomes to observe how people fix into the urbanization through morphological dynamics and the impact of collective memory on this transformation. The critical questions are: What is the conflict in SV? What is the relationship between collective memory and spatial contradictions in Shipai Village? What are the processes and outcomes of spatial contradictions? I argued that the contradiction in SV is a group clash, where cultural diversity has resulted in an unshared collective memory.AR2A011Architecture, Urbanism and Building Science
Inverse synthetic aperture radar imaging interface
No full textIn this project, the following two parts of work are mainly considered. The scope of the first part is to study the fundamental theory, particularly the imagery algorithm, for inverse synthetic aperture radar (ISAR) imaging, which is a powerful radar signal processing technique for high-resolution imagery of moving targets. The second part of the project is focused on the Matlab interface design for the simulation and demonstration of different ISAR imaging algorithms in various scenarios.
Firstly, the author reviews the theory of inverse synthetic aperture radar imaging technique, including imagery algorithms for both regular and maneuvering targets. The main task for the author on this part is to study the ISAR imaging technique and simulate the signal processing algorithms with Matlab. Subsequently, the algorithms are tested with simulated and real data.
Secondly, the Matlab interface design is also considered in this project. The graphical user interface (GUI) design procedures and results are presented to interactively demonstrate the performances of ISAR imagery algorithms on various dataset.
Finally, the author concludes this project and discusses the future work.Bachelor of Engineerin
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