25 research outputs found
Characterization of spliceosome assembly in cyanidioschyzon merolae.
No full textPre-mRNA splicing is the removal of intervening sequences from pre-messenger RNA in a reaction catalyzed by the spliceosome, which contains five small nuclear RNAs (snRNAs) and more than 100 proteins. Assembly of the spliceosome occurs in a highly ordered manner, making the spliceosome a very complex and dynamic particle. The spliceosome has been studied in yeast and humans but a simpler system would simplify splicing studies. Cyanidioschyzon merolae (Cm) has been shown to have a simpler spliceosome. The goal of this study was to characterize the Cm spliceosome beginning with the question of how large it is. To measure the size of the Cm spliceosome I used glycerol gradient centrifugation and assembly gels to study the assembly pathways. Lastly an attempt was made to study the components of Cm spliceosome by developing an assay in yeast (Saccharomyces cerevisiae) where small molecule inhibitors were used to stall the spliceososme, which could then be purified and its composition studied. --Leaf ii.The original print copy of this thesis may be available here: http://wizard.unbc.ca/record=b197654
Variation of Proximal Femoral Morphometry in Relation to Total Hip Replacement: A Study on Dry Femora
No full textObjective: Objective: Considerable variations exist in geometry of proximal femur across populations, which play an important role in total hip replacement surgeries. However, a rigorous characterization of femoral geometry across different geographical localities in Pakistan has not been performed. We aimed to assess the variations of the proximal femoral geometry in dry human femur bones from two different regions of Pakistan.
Study type settings & duration: A descriptive study was carried out at Islamic International Medical College, Rawalpindi and at three different Medical Colleges in Peshawar from October 2018 to July 2019.
Methodology: After obtaining ethical approvals, complete, fully ossified bones without additional paint or visible pathology from Rawalpindi (N = 98) and Peshawar (N = 112) were included for measurements of neck shaft angle (NSA), vertical head diameter and femoral neck length. NSA was measured from sketches of bones by using goniometer placed parallel to the shaft axis, while regular scale was used for measuring vertical head diameter and neck length. Unpaired T test was performed to compare the variations of femoral geometry in a side- and region-specific manner from Rawalpindi and Peshawar regions of Pakistan.
Results: Means and SD values of NSA, vertical diameter of head and neck length of all bones were 125.85˚± 7.35, 42.17±4.25 mm and 54.2±6mm, respectively. Significant differences (p value <0.001) were observed for NSA, vertical diameter of head and neck length between bones from the two regions. The femoral geometry was similar between right and left side bones, except for NSA in ......
Laughing with an Iranian American Woman: Firoozeh Dumas\u27s Memoirs and the (Cross-) Cultural Work of Humor
No full textThis essay critically analyzes Firoozeh Dumas\u27s humorous memoirs and situates them in the multiple contexts of post-9/11 Muslim American responses to Islamophobia, women\u27s humor, and Iranian American women\u27s life writing. Drawing on philosophical, feminist, ethnic, and contemporary scientific theories of humor and the methods of literary criticism, the author argues that Dumas employs the beneficial and inclusive (not malign and exclusive) positive mode of humorous personal storytelling to build connection through laughter via the emotional and cognitive shifts structurally central to humor. Dumas addresses multiple audiences and engages in important (cross-) cultural work in a particularly fraught political and cultural climate of anti-Muslim sentiment and tense Iran-U.S. relations
Colonizing Kashmir: state-building under Indian occupation Colonizing Kashmir: state-building under Indian occupation , by Hafsa Kanjwal, Stanford, Stanford University Press, 2023, xiii + 366 pp., $32, ISBN 978-1-5036-3603-3
No full textKashmiri life is expendable for the Indian state. While the love for the land is close to national imaginaries, the people have been subjected to decades of abuse and violence, and infringement of their basic human rights. In this book, Hafsa Kanjwal delves into the history of Kashmir, tracing the role of two pivotal political figures – Sheikh Abdullah (1947–1953) and Bakshi Ghulam Mohammad (1953–1963) – and their relationship with the Indian project of state-building in Kashmir. The author characterizes this as the ‘politics of life’ (9), where the Indian government and client regimes in Kashmir have normalized occupation with the propagation of ‘development, empowerment and progress’ along with bureaucratic integration and the forging of affective intimate relationships with the people of the state. However, the politics of life and appeals to emotions did not mean that there was an absence of coercive measures used by the Indian state to shape conforming and confronting subjectivities
Correlation of maternal neutrophil to lymphocyte ratio with fetal birth weight in preeclampsia
No full textPreeclampsia (PE) is a generalized inflammatory disorder complicating gestation, with most of cases developing in the third trimester. To evaluate the neutrophil to lymphocyte ratio (NLR) in diagnosed cases of preeclampsia and determine its correlation with fetal birth weight, a cross sectional analytical study was performed in Shaikh Zayed Medical Complex and Jinnah Hospital, Lahore. The participants included 60 cases of preeclampsia and 60 normotensive controls in third trimester of pregnancy. All the participants were in the age group of 20-40 years and BMI of 18-25.Total and differential leukocyte count was checked by automated hemoanalyzer and neutrophil lymphocyte ratio was calculated. Fetal birth weight was recorded immediately after birth. Statistical analysis was performed by SPSS (version 20). The p value of <0.05 was considered significant. NLR was significantly high (p-value < 0.001) in the preeclamptic group with a median value of 2.64(2.0-3.8) as compared to 1.87(1.7-2.0) in the healthy group. The fetal birth weight was significantly low (p-value < 0.001) in the pregnancies complicated with preeclampsia. High NLR was negatively correlated with fetal birth weight in preeclampsia (p-value < 0.01). On logistic regression significant association of high NLR with low fetal birth weight was found after controlling for the confounder gestational age, with an Odds ratio (OR) of 8.28 ( 95% confidence Interval 2.84-24.17). The area under the Receiver operating characteristic (ROC) curve for NLR was 0.8. The optimum cutoff value of NLR was 2.3 with 79% sensitivity and76% specificity. High maternal NLR in the third trimester in preeclampsia negatively correlates with fetal birth weight and is a simple promising biomarker to predict low fetal birth weight
Evaluation of salivary parameters (flow rate, pH, amylase and total protein) in periodontitis patients based on CPITN criteria
No full textIntroduction: The available literature on the topic is inconclusive and sometime taking less parameters in consideration. Moreover, there is no published data available in local population. Objectives: To determine and compare salivary flow rate, pH, total proteins, and alpha amylase in periodontitis patients based on community periodontal index of treatment needs (CPITN) score. Materials & Methods: A cross sectional study on 80 participants selected by purposive sampling was completed in 6 months. Participants were divided into 5 groups by CPITN scoring. Individuals more than 30 years with at least 20 teeth having calculus/staining were included. The study was conducted at Dental department of Civil Dispensary Peshawar city, Khyber College of Dentistry (periodontology ward) and Khyber Medical University (Biochemistry lab). After explaining the procedure and taking consent, saliva was collected from the participants following aseptic technique and analyzed through ELISA. Data was analyzed using SPSS version 22. P value <0.05 was considered to be significant in our study. Results: Among 80 subjects, 51 were females and 29 were males with mean age 35.73±7.05 years and BMI 26.47±5.23
Diagnostic Accuracy of Increased Total Leucocyte Count (TLC) And Decreased Absolute Neutrophil Count (ANC) For Diagnosis Of Neonatal Sepsis Taking Culture Sensitivity As Gold Standard
No full textObjective: To find diagnostic accuracy of increased total leucocyte count (TLC) and decreased absolute neutrophil count (ANC) for diagnosis of neonatal sepsis taking culture and sensitivity as gold standard.Methodology: Samples was collected from all the neonates with suspicion of sepsis admitted to neonatal due to severe bad health condition in the Children Hospital, Lahore. Parents were informed for consent. Total 3.5 cc sample was drawn by venipuncture.2.0 cc in CBC voil containing trisodium citrate, this sample was sent to CBC lab for automated CBC on sysmex KX-21 analyzer and peripheral smear. 1.5 cc was taken in specialized containers for blood culture and was sent to microbiology lab. Neonatal sepsis on TLC, ANC and blood count were be recorded. Patients of sepsis was managed as per hospital protocol. Data was analyzed using SPSS.Results: Neonates in this study was at mean age of 3.86±1.81 days. There was almost equal number of males and females neonates. Male were 155(51.7%) and female babies 145(48.3%). It was noted that 182(60.7%) babies were on term while 118(39.3%) were preterm neonates in this study. Neonatal sepsis was positive on TLC in 101(33.7%) cases. in 115(38.3%) on ANC.It was noted that sensitivity of the TLC was 71% while specificity was 66% with a diagnostic accuracy of 68%. On the other and, ANC sowed a sensitivity as 65%, specificity as 56% and diagnostic accuracy of 65%.Conclusion: Conclusively, TLC and ANC is not a good marker for the taking a neonate for the consideration of neonatal sepsis
Readiness for Hospital Discharge - Adult Patient Form - Urdu Translation
No full textThe Readiness for Hospital Discharge Scale, Quality of Discharge Teaching Scale, and Post-Discharge Coping Difficulty Scale were developed by Dr. Marianne Weiss to measure nurse-sensitive discharge care processes and patient outcomes. Three versions of the scales were developed concurrently for different patient populations – Adult medical-surgical patients, Parents of hospitalized children, and Postpartum Patient mothers (see Weiss & Piacentine, 2006, Weiss et al., 2007, and Bobay et al., 2018 for description of the development and initial testing). Additional scales and information about translation rights are located at Marquette Nursing. PERMISSION TO USE
The Readiness for Discharge Scale, Quality of Discharge Teaching Scale, and Post-Discharge Coping Difficulty Scale are available, and permission is granted to use the scales obtained from this website under the following conditions: The scales may not be modified or adapted. The scales may be used for research or for clinical practice. Permission is required from Dr. Weiss to load the scale(s) into the electronic health record and for hospital wide use of the scales. The scales may not be used or incorporated into for-profit/commercial programs. In publications reporting use of the scales, please reference Dr Weiss as the author of the scale and the translator (for non-English Scales) if noted on the scale form. The scales may not be published in manuscripts – only the results of use can be published. You may cite this website as the source of the scales. On publication of results, please send Dr Weiss a copy of the published paper.
No further permission is needed if you are using the scales under the above conditions. As a courtesy, please notify the translator listed on the website.
You must contact Dr Weiss directly ( [email protected]) for use in electronic health record system, hospital or system-wide use or for other purposes not listed above.
To see the scoring rubric, please go to https://www.marquette.edu/nursing/readiness-hospital-discharge-scale.ph
Adult QDTS, Urdu
No full textThe Readiness for Hospital Discharge Scale, Quality of Discharge Teaching Scale, and Post-Discharge Coping Difficulty Scale were developed by Dr. Marianne Weiss to measure nurse-sensitive discharge care processes and patient outcomes. Three versions of the scales were developed concurrently for different patient populations – Adult medical-surgical patients, Parents of hospitalized children, and Postpartum Patient mothers (see Weiss & Piacentine, 2006, Weiss et al., 2007, and Bobay et al., 2018 for description of the development and initial testing). Additional scales and information about translation rights are located at Marquette Nursing. PERMISSION TO USE
The Readiness for Discharge Scale, Quality of Discharge Teaching Scale, and Post-Discharge Coping Difficulty Scale are available, and permission is granted to use the scales obtained from this website under the following conditions: The scales may not be modified or adapted. The scales may be used for research or for clinical practice. Permission is required from Dr. Weiss to load the scale(s) into the electronic health record and for hospital wide use of the scales. The scales may not be used or incorporated into for-profit/commercial programs. In publications reporting use of the scales, please reference Dr Weiss as the author of the scale and the translator (for non-English Scales) if noted on the scale form. The scales may not be published in manuscripts – only the results of use can be published. You may cite this website as the source of the scales. On publication of results, please send Dr Weiss a copy of the published paper.
No further permission is needed if you are using the scales under the above conditions. As a courtesy, please notify the translator listed on the website.
You must contact Dr Weiss directly ( [email protected]) for use in electronic health record system, hospital or system-wide use or for other purposes not listed above.
To see the scoring rubric, please go to https://www.marquette.edu/nursing/hospital-discharge-scales-quality-of-discharge-teaching-scale.ph
