3 research outputs found
Moni se kulkee kontin kanss' (4/4 a)
No full textLaulun sanat: Moni se kulkee kontin kanss', Ajattelee juttuja juostessans'
Political Marketing: A Conceptual framework
No full textThe article presents a broad overview of the concept of political marketing and its significance in the contemporary era of information revolution and democratic resurgence. The article provides meaning, definition and various dimensions of political marketing as a concept and method and differentiates it from mainstream marketing practices. The article also attempts to analyze the origin and development of the concept in different political and social contexts and its usage as a powerful instrument in election campaigning and policy making. The article explains the eight main functions of political marketing including product function, distribution function, cost function, communication function, new management function, fund raising function, parallel campaign management function and internal cohesion management function. The article also explains how the functions of political marketing are effectively applied on the electoral market and government markets. The article finally examines the success and failure of political marketing in creating public opinion in favor of the party. Here examples are taken from different countries including both democratic and non-democratic countries.Political marketing;political;election;campaign;political communication
Structure of a 14-3-3ε:FOXO3a<sup>pS253</sup> Phosphopeptide Complex Reveals 14-3‑3 Isoform-Specific Binding of Forkhead Box Class O Transcription Factor (FOXO) Phosphoproteins
No full textThe transcriptional
activity of Forkhead Box O3 (FOXO3a) is inactivated
by AKT-mediated phosphorylation on Serine 253 (S253), which enables
FOXO3a binding to 14-3-3. Phosphorylated FOXO3a binding to 14-3-3
facilitates the nuclear exclusion of FOXO3a, causing cancer cell proliferation.
The FOXO3a/14-3-3 interaction has, therefore, emerged as an important
therapeutic target. Here, we report a comprehensive analysis using
fluorescence polarization, isothermal titration calorimetry, small-angle
X-ray scattering, X-ray crystallography, and molecular dynamics simulations
to gain molecular-level insights into the interaction of FOXO3apS253 phosphopeptide with 14-3-3ε. A high-resolution
structure of the fluorophore-labeled FOXO3apS253:14-3-3ε
complex revealed a distinct mode of interaction compared to other
14-3-3 phosphopeptide complexes. FOXO3apS253 phosphopeptide
showed significant structural difference in the positions of the -3
and -4 Arg residues relative to pSer, compared to that of a similar
phosphopeptide, FOXO1pS256 bound to 14-3-3σ. Moreover,
molecular dynamics studies show that the significant structural changes
and molecular interactions noticed in the crystal structure of FOXO3apS253:14-3-3ε are preserved over the course of the simulation.
Thus, this study reveals structural differences between the binding
to 14-3-3 isoforms of FOXO1pS256 versus FOXO3apS253, providing a framework for the rational design of isoform-specific
FOXO/14-3-3 protein–protein interaction inhibitors for therapy
