1,721,132 research outputs found
Analysis of chromosome aberrations in somatic and germ cells of the mouse
No full textChromosome aberration tests are used to evaluate the clastogenicity of chemical and physical agents, that is, the capacity of these agents to cause breaks in chromosomes and produce microscopically visible fragments or structural rearrangements. Aberrations are scored in metaphase chromosomes of dividing cells. In the mouse, bone marrow progenitors of erythrocytes and leukocytes provide abundant metaphases to study the effects on somatic cells, whereas the response of male germ cells to clastogenic agents can be visualized on metaphases of spermatogonia and primary spermatocytes. The techniques to prepare the slides for analyses are well standardized and internationally harmonized protocols for tests in bone marrow and spermatogonia provide the guidance necessary to obtain meaningful results. It is advisable to adhere as much as possible to these recommendations. Not all tests are suitable to score the same kind of aberrations. Here an overview of the application domains of these tests is provided with warnings on the scoring criteria and statistical analysis. © Springer Science+Business Media, New York 2013
Le zeoliti, minerali possibili indicatori di fenomenologie idromagmatiche; un'ipotesi di lavoro
No full textStudies on the use of sepharose-N-aminohexanoyl-glucosamine for purification of rabbit red blood cell hexokinase.
No full textDetermination of the energy load of erythrocytes in long-distance and medium-distance runners
No full textIn this study erythrocytes drawn from well-trained athletes (middle- and long-distance runners) and from sedentary subjects have been compared for their adenine nucleotide contents. ADP and AMP appeared to be significantly (p less than 0,001) increased only in red cells from athletes in the rest state. After athletes' race this difference with control subjects become insignificant. Nevertheless, the observed ADP and AMP modifications are not great enough to influence the energy charge (CE) of the compared erythrocytes
Morphological and molecular characterisation of mycelia of ectomycorrhizal fungi in pure culture
No full textBoletus edulis, Boletus aestivalis, Boletus luridus, Amanita muscaria and Hebeloma radicosum mycelia were isolated in pure culture and characterised by morphological and molecular methods. Molecular identification was performed by sequence analyses from the ITS region of nuclear ribosomal RNA genes. The phylogenetic affiliation of the isolated mycelia were evaluated by comparison of their ITS sequences with those deposited in the GenBank database. Pure cultures of isolates of the different fungal genera under investigation showed differences in growth rate, colony morphology and/or hyphal biometric characters. In contrast, the morphological characteristics of the mycelia of the three Boletus species were similar, but these species were distinguished by ITS data. Problems remain, however, in the affiliation of these ITS sequences with those of the B. edulis group that are currently deposited in public databases
Fungal-bacterial interaction within the ectomycorrhizal fungus Tuber borchii Vittad. life cycle
No full text
Morphological and molecular characterization of mycelia of some Tuber species in pure culture
No full textPure cultures of Tuber maculatum, Tuber melanosporum, Tuber aestivum, Tuber macrosporum, Tuber rufum and Tuber brumale were isolated and characterized by morphological and molecular methods. The Tuber mycelia were isolated from fruit bodies and molecular identification was performed using specific primers, restriction fragment length polymorphism and/or sequence analyses of the ITS region. The species grew between 1.1 mm wk-1 and 14 mm wk-1 on the selected medium. The mycelium of different Tuber species showed several common morphological features such as hyphal anastomoses, vesicle formation and hyphal aggregation. Differences were found in the frequency of these morphological features and in the hyphal pattern. The isolated mycelia also showed differences in the hyphal branch angle, septal distance, hyphal diameter and rate of growth of the hyphae. This result opens the possibility of using pure mycelial cultures of Tuber spp. for experimental purposes and for the commercial production of infected truffle plants. © New Phytologist (2002)
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