289,398 research outputs found
Bill T. Jones Still/Here
No full textA look at dancer and choreographer Bill T. Jones's highly acclaimed dance: Still/Here. At workshops around the country, people facing life-threatening illnesses are asked to remember the highs and lows of their lives, and even imagine their own deaths. They then transform these feelings into expressive movement, which Jones incorporates into the dance Still/Here. Jones demonstrates the movements of his life story: his first encounter with white people, confusion over his sexuality, his partner Arnie Zane's untimely death from AIDS, and Jones's own HIV status.Danced by The Bill T. Jones/Arnie Zane Dance Company. Cinematography, Joel Shapiro and Don Lenzer ; edited by Geof Bartz ; music & lyrics for Still / by Kenneth Frazelle ; sung by Odetta. Music for Here / composed and arranged by Vernon Reid
Ely, H.P., letter, Medford, [N.J.], November 15, 1857, to "Resp[ec]t[e]d F[rien]d." [Peter Still]
No full textH.P. Ely informs Peter Still that he (Ely) has received a letter from Dillwyn Smith; alludes to a trip to New England by Still to sell books; reports that Smith is suffering financially, apparently as arbitration (relating to construction of Still’s house?) reduced the amount of money that Smith was to receive, despite related bills that Smith must still pay; believes that Still should pay one of Smith’s relevant bills, as Smith “has met with many losses” and “Dillwyn[’]s family & connections have done more for [him] than all others with their money & reccommendations [sic]”; further suggests that through inaction Still could “loose the interest of [his] best friends and injure the cause of freedom for the slave”; and adds, in a postscript, that he has forgotten to mention a bill from the painter, who urgently seeks payment
Low back pain, somatic dysfunction, and segmental bone mineral density T-score variation in the lumbar spine
No full textContext: Identifying objective measures that correlate with somatic dysfunction palpatory findings will aid in establishing clinical relevance of the findings and provide outcome measures for future studies. Objective: To investigate the association of altered segmental lumbar vertebral mechanics (ie, somatic dysfunction) as assessed by palpation with bone mineral density (BMD) Tscore variability in participants, some with chronic low back pain (CLBP) and others without low back pain (LBP). Methods: Individuals with CLBP and individuals without LBP were examined by 2 blinded examiners for the presence or absence of paraspinal tissue texture abnormalities, vertebral rotational asymmetry, anterior motion restriction, and tenderness from L1 to L4. All participants then received a dualenergy x-ray absorptiometry scan of the lumbar spine. Bone mineral density T scores were compared between the CLBP and non-LBP groups. Results: Sixty-three individuals (16 CLBP, 47 non-LBP) participated in the study. Lumbar segments with perceivable rotational asymmetry had higher mean BMD T scores (95% confidence interval [95% CI]) than lumbar segments with no asymmetry (0.5 [0.4-0.7] vs -0.2 [-0.6 to 0.2], respectively; P=.002). Additionally, lumbar segments with anterior motion restriction had higher mean BMD T scores (95% CI) than lumbar segments with no motion restriction (0.6 [0.4-0.7] vs 0.1 [-0.2 to 0.3], respectively; P=.03). Participants with CLBP demonstrated higher regional mean lumbar BMD T scores (95% CI) than those without CLBP (0.9 [0.6-1.1] vs 0.3 [0.2-0.5], respectively; P\u3c.001). After accounting for sex and body mass index, vertebral segments with rotational asymmetry (in non- LBP participants only) and vertebral segments with motion restriction had higher mean BMD T scores than vertebral segments with no asymmetry or motion restriction. Conclusion: Participants with CLBP had significantly higher lumbar BMD than participants without LBP. The presence of rotational asymmetry or motion restriction was associated with elevated BMD at the affected vertebrae
Low back pain, somatic dysfunction, and segmental bone mineral density T-score variation in the lumbar spine
No full textContext: Identifying objective measures that correlate with somatic dysfunction palpatory findings will aid in establishing clinical relevance of the findings and provide outcome measures for future studies. Objective: To investigate the association of altered segmental lumbar vertebral mechanics (ie, somatic dysfunction) as assessed by palpation with bone mineral density (BMD) Tscore variability in participants, some with chronic low back pain (CLBP) and others without low back pain (LBP). Methods: Individuals with CLBP and individuals without LBP were examined by 2 blinded examiners for the presence or absence of paraspinal tissue texture abnormalities, vertebral rotational asymmetry, anterior motion restriction, and tenderness from L1 to L4. All participants then received a dualenergy x-ray absorptiometry scan of the lumbar spine. Bone mineral density T scores were compared between the CLBP and non-LBP groups. Results: Sixty-three individuals (16 CLBP, 47 non-LBP) participated in the study. Lumbar segments with perceivable rotational asymmetry had higher mean BMD T scores (95% confidence interval [95% CI]) than lumbar segments with no asymmetry (0.5 [0.4-0.7] vs -0.2 [-0.6 to 0.2], respectively; P=.002). Additionally, lumbar segments with anterior motion restriction had higher mean BMD T scores (95% CI) than lumbar segments with no motion restriction (0.6 [0.4-0.7] vs 0.1 [-0.2 to 0.3], respectively; P=.03). Participants with CLBP demonstrated higher regional mean lumbar BMD T scores (95% CI) than those without CLBP (0.9 [0.6-1.1] vs 0.3 [0.2-0.5], respectively; P\u3c.001). After accounting for sex and body mass index, vertebral segments with rotational asymmetry (in non- LBP participants only) and vertebral segments with motion restriction had higher mean BMD T scores than vertebral segments with no asymmetry or motion restriction. Conclusion: Participants with CLBP had significantly higher lumbar BMD than participants without LBP. The presence of rotational asymmetry or motion restriction was associated with elevated BMD at the affected vertebrae
Benign tumors in TSC are amenable to treatment by GD3 CAR T cells in mice
No full textMutations underlying disease in tuberous sclerosis complex (TSC) give rise to tumors with biallelic mutations in TSC1 or TSC2 and hyperactive mammalian target of rapamycin complex 1 (mTORC1). Benign tumors might exhibit de novo expression of immunogens, targetable by immunotherapy. As tumors may rely on ganglioside D3 (GD3) expression for mTORC1 activation and growth, we compared GD3 expression in tissues from patients with TSC and controls. GD3 was overexpressed in affected tissues from patients with TSC and also in aging Tsc2+/- mice. As GD3 overexpression was not accompanied by marked natural immune responses to the target molecule, we performed preclinical studies with GD3 chimeric antigen receptor (CAR) T cells. Polyfunctional CAR T cells were cytotoxic toward GD3-overexpressing targets. In mice challenged with Tsc2-/- tumor cells, CAR T cells substantially and durably reduced the tumor burden, correlating with increased T cell infiltration. We also treated aged Tsc2+/- heterozygous (\u3e60 weeks) mice that carry spontaneous Tsc2-/- tumors with GD3 CAR or untransduced T cells and evaluated them at endpoint. Following CAR T cell treatment, the majority of mice were tumor free while all control animals carried tumors. The outcomes demonstrate a strong treatment effect and suggest that targeting GD3 can be successful in TSC
DNA sequence analysis of spontaneous and γ-radiation (anoxic)-induced lacI\u3csup\u3ed\u3c/sup\u3e mutations in Escherichia coli umuC122::Tn5: differential requirement for umuC at G · C vs. A · T sites and for the production of transversions vs. transitions
No full textEscherihica coli umC122::Tn5 cells were γ-radiated (137Cs, 750 Gy, under N2), and lac-constitutive mutants were produced at 36% of the wild-type level (the umC strain was not deficient in spontaneous mutagenesis, and the mutational spectrum determined by sequencing 263 spontaneous lacId mutations was very similar to that for the wild-type strain). The specific nature of the umC strain\u27s partial radiation was determined by sequencing 325 radiation-induced lacId mutations. The yields of radiation-induced mutation classes in the umC strain (as a percentage of the wild-type yield) were: 80% for A · T → G · C transitions, 70% for multi-base additions, 60% for single-base deletions, 53% for A · T → C · G transversions, 36% for G · C → A · T transitions, 25% for multi-base deletions, 21% for A · T → T · A transversions, 11% for G · C → C · G transversions, 9% for G · C → T · A transversions and 0% for multiple mutations. Based on these deficiencies and other factors, it is concluded that the umC strain is near-normal for A · T → G · C transitions, single-base deletions and possibly A · T → C · G transversions; is generally deficient for mutagenesis at G · C sites fro transversions, and is grossly deficient in multiple mutations. Damage at G · C sites seems more difficult for translesion DNA synthesis to bypass than damage at A · T sites, and especially when trying to produced a transversion. The yield of G · C → A · T transitions in the umC strain *36% of the wild-type level) argues that a basic sites are involved in no more than 64% of γ-radiation-induced base substitutions in the wild-type strain. Altogether, these data suggest that the UmuC and UmuD′ proteins facilitate, rather than being absolutely required for, translesion DNA synthesis; with the degree of facilitation being dependent both on the nature of the noncoding DNA damage, i.e., at G · C vs A · T sites, and on the nature of the misincorporated base, i.e., whether it induces transversions or transitions. © 1994
Cytokine array analysis of mediators produced by human macrophages stimulated with Trichomonas tenax
No full textThe incidence of oral colonization by the protozoan Trichomonas tenax correlates with gingival inflammation and periodontitis in humans. To determine whether T. tenax might contribute to inflammation by eliciting cytokines from human cells, differentiated THP-1 (dTHP-1) macrophages were cultured with live or sonicated T. tenax trophozoites, and the conditioned media were assayed for 36 different mediators by a membrane-based cytokine array. Scanning densitometry of the membranes revealed that live T. tenax trophozoites stimulated secretion of interleukin-8 (IL-8), macrophage migration inhibitory factor (MIF), IL-1β, intercellular adhesion molecule-1 (ICAM-1), and IL-1 receptor antagonist (IL-1ra) from dTHP-1 macrophages. T. tenax lysates stimulated release of IL-8, MIF, and IL-1ra. Despite often being classified as a commensal organism, T. tenax elicited a wider variety of cytokines than the human urogenital pathogen, T. vaginalis, which elicited only IL-8 and MIF production from dTHP-1 cells
Interobserver reliability of osteopathic palpatory diagnostic tests of the lumbar spine: Improvements from consensus training
No full textContext: Establishing reliable palpatory tests continues to be a critical, yet elusive, step in osteopathic medical research and evidence-based clinical practice. Objective: The authors investigated the interobserver reliability of common osteopathic palpatory tests used to evaluate the lumbar spine. Design and Methods: Subjects (N=119) were recruited from the faculty, staff, and students of Kirksville (Mo) College of Osteopathic Medicine (KCOM) of A.T. Still University of Health Sciences. Three osteopathic medical examiners residency trained in neuromusculoskeletal medicine initially evaluated lumbar segments on subjects from one subgroup (n=42) in a blinded assessment. The examiners performed palpatory tests of tenderness and tissue texture changes, as well as-in three planes-vertebral positional asymmetry and motion asymmetry. Kappa statistics (κ) were used to evaluate interobserver reliability. Following a period of consensus training, subjects from another subgroup (n=77) were evaluated in a blinded assessment for those palpatory tests that seemed most likely to produce reliable findings. The interobserver reliability was then re-evaluated. Results: During the initial evaluation of interobserver reliability, κ ranged from -0.02 to 0.34, within the poor-to-fair reliability range. Following consensus training, reliability improved, rising into the moderate range for tissue texture changes (κ=0.45) and into the substantial range for tenderness assessments (κ=0.68). Reliability for positional asymmetry in the transverse plane (κ=0.34) and rotational motion asymmetry (κ=0.20) were improved but remained in the fair range. Conclusion: The authors concluded that consensus training improved the interobserver reliability of common osteopathic palpatory tests of the lumbar spine. In two of the four tests that were studied-tissue texture and tenderness-acceptable κ values for clinical tests were achieved after consensus training
DNA sequence analysis of γ-radiation (anoxic)-induced and spontaneous lacI\u3csup\u3ed\u3c/sup\u3e mutations in Escherichia coli K-12
No full textAn extensive spectrum of ionizing radiation mutagenesis was determined by sequencing 318 137Cs γ-radiation (anoxic)-induced episomal lacId mutations in Escherichia coli strain NR9102. The most commonly found radiation-induced mutations were based substitutions (44% transversions and 41% transitions). The radiation-induced spectrum consisted of: 23% G · C → A · T, 18% A · T → G · C, 17% G · C → T · A, 14% G · C → C · G, 8% A · T → T · A, 6% A · T → C · G, 8% single-based deletions, 5% multiple mutations, 3% multi-base deletions, and essentially no single-or multi-base additions. This spectrum compared better with spectra for other systems obtained by in vivo irradiation than with one obtained by in vitro irradiation. Multiple mutations, which were unique to the radiation-induced spectrum, generally consisted of one active and one closely linked silent mutation and are suggested to result from an altered replication complex of reudced fidelity. Mutation rates were 4.1 × 10-8 lac-constitutive mutations/gene/Gy and 1.2 × 10-10 base substitutions/base pair/Gy. Thirty-two percent more radiation-induced mutations occurred at G · C vs. A · T base pairs. A strand asymmetry was noted for G · C → C · G and A · T → T · A transversions. A nearest-neighbor analysis showed that C (vs. A, G. or T), on either side of the mutation site, substantially enhanced most types of base substitutions. Similarly, G and C flanked both sides of single-base deletion sites twice as frequently as would be expected from the base composition of the mutation target. For comparative purpose, we sequenced 411 spontaneous lac-constitutive mutants of which 269 were lacId mutants, and there was good agreement between these and previously published mutational spectra. The spontaneous and radiation-induced mutational spectra differed substantially for virtually every class of mutation. For example, the set of spontaneous dominant lac-constitutive mutations contained many more mutations that did not map in the normal region for lacId mutations (i.e., 35% vs. 3%) and were presumed to be lacO-constitutive mutations. A sampling of these presumptive lacOc mutations was also sequenced: 17 22 (spontaneous) and 1 9 (radiation) were found to be lacOc long deletions, one from each set were base substitutions, and the remaining mutations showed the wild-type lacO sequence. Like the radiation-induced spectrum, the spontaneous spectrum showed enhanced mutagenesis at G · C sites, strand asymmetry, and enhanced mutagenesis when G or C were the nearest neighbors. © 1994
Effect of Phytochemical Compounds on Trichomonas tenax, an Oral Protozoan
No full textAbstract
Trichomonas tenax is an oral protozoan with an estimated global pooled prevalence of 17% in the human population.1 Observational studies have demonstrated a significant statistical correlation between oral colonization by T. tenax and the progression of periodontal disease.2 Proposed pathogenic mechanisms for this protozoan include the production of tissue-damaging enzymes, induction of apoptosis in human cells, and dysbiosis of the oral microbiome.3 In patients for whom metronidazole (MTZ) is contraindicated, phytochemicals may offer a viable alternative for controlling T. tenax. Various plant extracts have shown promising in vitro activity against other trichomonads, such as T. vaginalis and Tritrichomonas foetus, as reviewed by Friedman et al.4
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