642 research outputs found
Solvent/detergent treated Cohn extract on 4.2 L Hupresin.
No full textSolvent/detergent treated Cohn extract on 4.2 L Hupresin.</p
Summary of HuBChE yield from Cohn paste extract purified on 30 L of Q-Ceramic<sup>a</sup>.
No full textSummary of HuBChE yield from Cohn paste extract purified on 30 L of Q-Ceramica.</p
Purification of human butyrylcholinesterase from frozen Cohn fraction IV-4 by ion exchange and Hupresin affinity chromatography.
No full textHuman butyrylcholinesterase (HuBChE) is being developed as a therapeutic for protection from the toxicity of nerve agents. An enriched source of HuBChE is Cohn fraction IV-4 from pooled human plasma. For the past 40 years, purification of HuBChE has included affinity chromatography on procainamide-Sepharose. The present report supports a new affinity sorbent, Hupresin, for purification of HuBChE from Cohn fraction IV-4. Nine batches of 70-80 kg frozen Cohn fraction were extracted with water, filtered, and chromatographed on 30 L of Q-Ceramic ion exchange sorbent at pH 4.5. The 4% pure Q-eluent was pumped onto 4.2 L Hupresin, where contaminants were washed off with 0.3 M NaCl in 20 mM sodium phosphate pH 8.0, before 99% pure HuBChE was eluted with 0.1 M tetramethylammonium bromide. The average yield was 1.5 g of HuBChE from 80 kg Cohn paste. Recovery of HuBChE was reduced by 90% when the paste was stored at -20°C for 1 year, and reduced 100% when stored at 4°C for 24h. No reduction in HuBChE recovery occurred when paste was stored at -80°C for 3 months or 3 years. Hupresin and procainamide-Sepharose were equally effective at purifying HuBChE from Cohn fraction. HuBChE in Cohn fraction required 1000-fold purification to attain 99% purity, but 15,000-fold purification when the starting material was plasma. HuBChE (P06276) purified from Cohn fraction was a 340 kDa tetramer of 4 identical N-glycated subunits, stable for years in solution or as a lyophilized product
The Proposed Federal Rules of Appellate Procedure
Get PDFAfter a discussion of the history of uniform procedural rules and the authority of the Supreme Court to promulgate uniform appellate rules, Professor Cohn discusses some of the problems of expense and delay which are found in the appellate system today. In his analysis of the Proposed Uniform Rules of Federal Appellate Procedure, the author gives special emphasis to those areas where the rules constitute a departure from present practice. In addition, several changes are suggested in areas in which the author believes further improvement can be made
The Work-Product Doctrine: Protection, Not Privilege
Get PDFAlthough the work-product doctrine has received considerable attention before the courts in recent years, several issues regarding the scope and applicability of the doctrine remain controversial As a prelude to explaining the state of the law on these issues, the author examines the case law through which the doctrine developed and explores the doctrine\u27s modern application through rule 26 of the Federal Rules of Civil Procedure. He next discusses the rule\u27s various requirements and its treatment ofparticular categories of information including opinion work product andparty statements. Finally, Professor Cohn explains how the rule\u27s protection may be waived and discusses the rule\u27s operation with respect to subsequent litigation and aparty\u27s use of experts. The author draws distinctions throughout the article between operation of the attorney- client privilege and the work-product doctrine and concludes that the work-product doctrine operates not as aprivilege that belongs to any party but rather as a protection for the adversary systetr
Efficacy of arsenic exposure reduction via drinking water treatment systems
No full textArsenic, a known human carcinogen, exceeds the maximum contaminant level in New Jersey private wells at a higher percentage than any other contaminant with a primary drinking water standard. New Jersey’s drinking water standard for arsenic at 5 µg/L is currently the most protective in the world. Water treatment systems can remove arsenic from drinking water, either from the entire home (point-of-entry) or just at a single tap (point-of-use) for drinking and cooking. The goal of this research was to compare human exposure to arsenic between point-of-entry and point-of-use water treatment, by biomonitoring, to determine which level of treatment most effectively reduced arsenic exposure and dose from water at home to acceptable risk levels. The study recruited 53 subjects in 22 households obtaining arsenic water treatment, and five control subjects with little or no measurable arsenic in their water supply. The mean arsenic concentration in untreated water was 44 µg/L. Biomonitoring started before initiation of water treatment and continued for up to three years with samples analyzed at the Environmental and Occupational Health Sciences Institute. The study determined that: 1) dietary arsenic can be a major confounder in arsenic biomonitoring studies; 2) arsenic speciation techniques are extremely valuable for arsenic biomonitoring studies; 3) sampling protocols and reference values for arsenic in urine and blood should be recommended; 4) arsenic water treatment systems are effective in reducing arsenic exposure from well water; 5) there is a measurable arsenic body burden after chronic exposure to arsenic in drinking water; 6) there is a two-compartment clearance of arsenic from urine, after cessation of ingesting the arsenic contaminated water; and 7) after nine months of water treatment, the adjusted mean inorganic-related arsenic concentrations in urine were significantly lower in the point-of-entry treatment group with a mean ± standard error of 2.7 ± 0.6 µg/g creatinine than in the point-of-use treatment group at 6.1 ± 0.7 µg/g creatinine. In conclusion, point-of-entry treatment of arsenic-contaminated well water should be recommended in preference to point-of-use.Ph.D.Includes abstractVitaIncludes bibliographical referencesby Steven E. Spay
Decomposition of 1,1-Dichloroethane and 1,1-Dichloroethene in an electron beam generated plasma reactor
Get PDFAn electron beam generated plasma reactor is used to decompose low concentrations (100–3000 ppm) of 1,1-dichloroethane and 1,1-dichloroethene in atmospheric pressure air streams. The energy requirements for 90% and 99% decomposition of each compound are reported as a function of inlet concentration. Dichloroethene decomposition is enhanced by a chlorine radical propagated chain reaction. The chain length of the dichloroethene reaction is estimated to increase with dichloroethene concentration from 10 at 100 ppm initial dichloroethene concentration to 30 at 3000 ppm. Both the dichloroethane and dichloroethene reactions seem to be inhibited by electron scavenging decomposition products. A simple analytic expression is proposed for fitting decomposition data where inhibition effects are important and simple first order kinetics are not observed
ICarbS, Volume 2, Issue 1
Get PDFContents
General Grant and Mark Twain by John Y. Simon / 3
Ben L. Reitman, M.D.: Portrait of a Pseudo-Anarchist by Paul E. Kuhl / 11
Brian O\u27Nolan: The Privacy of His Mind by Miles Orvell / 23
Borstal Revisited by Corey Phelps / 39
James Joyce and James Stephens: The Coincidence of the Second of February by Alan M. Cohn / 61
James Joyce Collections at Morris Library by Steven P. Lund and
Alan M. Cohn / 67
Illustrations
Line drawing of Samuel Clemens by Werner Mertz / 4
Samuel Clemens to Julia Dent Grant, 3 February 1886 / 6-8
Irish Times (dummy) crowns Myles King of Ireland / 24
Manuscript of O\u27Nolan article on James Joyce / 28
Typescript of O\u27N alan light poem on Ezra Pound / 32
Manuscript opening for The Courteous Borstal / 40
Typescript from Behan\u27s Bridewell Revisited / 48
Behan typescript, Deliverance / 54
James Stephens\u27 Sarasvati / 62
Minuette, with comments by Stephens / 64
John Sullivan, James Joyce and James Stephens in Paris / 6
- …
