1,721,130 research outputs found
Stem cells and future periodontal regeneration
No full textNi-Hung Lin, Stan Gronthos and P. Mark Bartol
Cellular therapy for cardiovascular disease Part 2 - Delivery of cells and clinical experience
Full text linkPeter J Psaltis, Stan Gronthos, Stephen G Worthley and Andrew C.W. Zannettin
Cellular therapy for cardiovascular disease Part 1 - Preclinical insights
Full text linkPeter J Psaltis, Stan Gronthos, Stephen G Worthley and Andrew C.W. Zannettin
Generation of Neural Crest‐Like Cells From Human Periodontal Ligament Cell‐Derived Induced Pluripotent Stem Cells
No full textAbstract not available.Atsushi Tomokiyo, Kim Hynes, Jia Ng, Danijela Menicanin, Esther Camp, Agnes Arthur, Stan Gronthos, and Peter Mark Bartol
A method to isolate and purify human bone marrow stromal stem cells
No full textThe original publication can be found at www.springerlink.comThe STRO-1 antibody can be used as a single reagent to isolate human bone marrow stromal stem cells (BMSSC), owing to its restricted specificity to a cell surface molecule expressed by clonogenic BMSSC, with little or no reactivity to hematopoietic stem/progenitor populations or mature stromal elements. The present protocol uses a combination of two different immunoselection methodologies in an attempt to generate highly purified preparations of BMSSC. This process involves the initial isolation of a minor subpopulation of bone marrow mononuclear cells (approx 10%) expressing the STRO-1 antigen, by means of magnetic activated cell sorting. Dual-color fluorescence activated cell sorting is then used as a secondary step to further purify the rare STRO-1bright expressing fraction that contains all of the colony-forming BMSSC, based on their co-expression of a secondary cell surface marker, CD106 (VCAM-1).Stan Gronthos and Andrew C. W. Zannettin
Circulating levels of TWEAK correlate with bone erosion in multiple myeloma patients
No full textSharon A. Williams, Sally K. Martin, Cristina Vincent, Stan Gronthos, Timothy Zheng, Gerald J. Atkins and Andrew C. W. Zannettin
Lineage differentiation of mesenchymal stem cells from dental pulp, apical papilla, and periodontal ligament
No full textRecently, a variety of mesenchymal stem cells (MSCs), including dental pulp stem cells, stem cells from human exfoliated deciduous teeth, stem cells from apical papilla, periodontal ligament stem cells, and mesenchymal stem cells derived from human gingival, were isolated from orofacial and dental tissues. However, it is unknown whether these orofacial stem cells are derived from mesoderm or neural crest cell. In order to encourage orofacial MSC investigation, we provide detailed protocols for assessing lineage differentiation of orofacial MSCs.Kentaro Akiyama, Chider Chen, Stan Gronthos, Songtao Sh
Regulation and differentiation potential of dental mesenchymal stem cells
No full textPublished Online: 26 MAR 2013This chapter summarizes the regulatory factors that influence the differentiation potential of dental mesenchymal stem cells (MSCs), including dental pulp stem cells (DPSCs), stem cells from human exfoliated deciduous teeth (SHEDs), periodontal ligament stem cells (PDLSCs), dental follicle cells (DFCs), stem cells from root apical papilla (SCAPs), and gingival-derived MSCs (GMSCs). Dental MSCs can be differentiated into multiple lineages and are summarized in a table. Studies have shown that several molecules are involved in the regulation of dental MSC differentiation. This chapter also focuses on the major signaling pathways involved in the modulation of differentiation potential in dental MSCs. In conclusion, the dental MSC differentiation is regulated intensively at the cellular, molecular, and transcriptional levels. © 2013 Wiley-Blackwell.Lei Wang, Christian Morsczeck, Stan Gronthos, Songtao Sh
Dental pulp stem cells
No full textPostnatal stem cells have been isolated from a variety of tissues. These stem cells are thought to possess great therapeutic potential for repairing damaged and/or defective tissues. Clinically, hematopoietic stem cells have been successfully used for decades in the treatment of various diseases and disorders. However, the therapeutic potential of other postnatal stem cell populations has yet to be realized, because of the lack of detailed understanding of their stem cell characteristics at the cellular and molecular levels. Furthermore, there is limited knowledge of their therapeutic value at the preclinical level. Therefore, it is necessary to develop optimal strategies and approaches to overcome the substantial challenges currently faced by researchers examining the clinical efficacy of different postnatal stem cell populations. In this review, we introduce methodologies for isolating postnatal stem cells from human dental pulp and discuss their potential role in tissue regeneration.He Liu, Stan Gronthos, and Songtao Sh
A method to isolate, purify, and characterize human periodontal ligament stem cells
No full textHuman periodontal ligament stem cells (PDLSCs) are a unique population of mesenchymal stem cells (MSCs) which demonstrate the capacity to generate cementum- and periodontal ligament-like structures in vivo. As such, PDLSCs represent a promising cell-based therapy in reconstructive dentistry for the treatment of periodontal disease. The present chapter describes two methods for isolating PDLSCs from human PDL tissue including traditional plastic adherence and immunomagnetic selection based on the expression of MSC-associated surface markers STRO-1 antigen, CD146 (MUC-18), CD29 (integrin beta-1), CD44, and CD106 (VCAM-1). Although no single antibody demonstrates specificity for MSCs, isolation based on the expression of individual markers results in homogeneous preparations of PDLSCs. Methods to further characterize the immunophenotype and multipotent capacity of PDLSCs to differentiate into adipocytes, osteoblast- and cementoblast-like cells in vitro, and cementum- and periodontal ligament-like tissues in vivo are also described.Krzysztof Mrozik, Stan Gronthos, Songtao Shi and P. Mark Bartoldhttp://trove.nla.gov.au/work/3866672
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