632 research outputs found
Gene regulation in psoriatic keratinocytes : microRNAs and cytokine responses
No full textPsoriasis is the most common chronic skin inflammation in adults which affects 2-4% of the world population. Keratinocytes are the key structural cells in epidermis which hyper-proliferate and undergo aberrant differentiation in psoriasis. Keratinocyte-derived cytokines and chemokines are essential in maintaining the vicious auto-inflammatory loop in psoriasis. MicroRNAs (miRNAs) are small RNAs (22-25 nucleotides) which regulates gene expression at the post-transcriptional level. In this thesis, we explored the functional role of miRNAs/ cytokine networks in psoriasis.Paper I: A strong protective association of SNP (rs2910164-CC) of miR-146a to early onset of psoriasis was observed in HLA-C*06-negative patients. In addition, miR-146a deletion in mice led to earlier onset, more severe inflammation and impaired healing of imiquimod-induced psoriasis-like skin inflammation. Therapeutically, miR-146a mimic injection prevented psoriasiform skin-inflammation in WT mice, suggesting an anti-inflammatory role of miR-146a.Paper II: Here we explored the landscape of miRNAs in psoriatic keratinocytes. We identified several miRNAs, which were not identified in psoriasis before and their expression was altered in keratinocyte. In addition, upregulation of miR-1307-3p and human-specific miR-941 was validated with qRT-PCR.Paper III: MiR-149 was found to be downregulated in psoriatic keratinocytes compared to keratinocytes from non-lesional or healthy skin. In vitro studies revealed that the downregulation of miR-149 is mediated by IFN-γ/JAK/STAT axis. Functionally, miR-149 targeted the inflammatory and JAK/STAT pathway genes in resting as well as in IFN-γ treated keratinocytes. Moreover, miR-149 inhibition potentiated IFN-γ responses in keratinocytes.Paper IV: Here we outlined a new mode of action of tofactinib, a JAK inhibitor with effects on psoriasis in clinical trials. We showed that JAK proteins that are targets of tofacitinib are expressed by keratinocytes and tofacitinib had a robust impact on IL-22- regulated transcriptome in keratinocytes. Our results imply that effects of tofacitinib are not just limited to T-cells as previously thought but can also be observed in keratinocytes.List of scientific papersI. MicroRNA-146a suppresses IL-17–mediated skin inflammation and is genetically associated with psoriasis. Ankit Srivastava, Pernilla Nikamo, Warangkana Lohcharoenkal, Dongqing Li, Florian Meisgen, Ning Xu Landén, Mona Ståhle, Andor Pivarcsi and Enikö Sonkoly. J Allergy Clin Immunol. 2016 Aug; 139(2): 550-560. https://doi.org/10.1016/j.jaci.2016.07.025 II. Next generation sequencing identifies the keratinocyte-specific miRNA signature of psoriasis. Ankit Srivastava, Florian Meisgen, Lorenzo Pasquali, Sara Munkhammar, Ping Xia, Mona Ståhle, Ning Xu Landén, Andor Pivarcsi and Enikö Sonkoly. [Submitted]III. miR-149 is downregulated in psoriasis and regulates the JAK-STAT pathway in keratinocytes. Ankit Srivastava, Florian Meisgen, Mona Ståhle, Andor Pivarcsi and Enikö Sonkoly. [Manuscript]IV. Tofacitinib represses the Janus Kinase-Signal Transducer and Activators of Transcription signalling pathway in keratinocytes. Ankit Srivastava, Mona Ståhle, Andor Pivarcsi and Enikö Sonkoly. Acta Derm Venereol. 2018 May; 98: 772–775. https://doi.org/10.2340/00015555-2960 </p
sj-docx-2-pie-10.1177_09544089231191905 - Supplemental material for Experimental investigation on process parameters induced mechanical and microstructural properties for laser powder bed fusion additive manufacturing of SS316L
No full textSupplemental material, sj-docx-2-pie-10.1177_09544089231191905 for Experimental investigation on process parameters induced mechanical and microstructural properties for laser powder bed fusion additive manufacturing of SS316L by Meet Gor, Harsh Soni, Nishkarsh Srivastava, Amit Arora, Pankaj Sahlot, Ankit Oza and Anita Gehlot in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering</p
sj-docx-1-pie-10.1177_09544089231191905 - Supplemental material for Experimental investigation on process parameters induced mechanical and microstructural properties for laser powder bed fusion additive manufacturing of SS316L
No full textSupplemental material, sj-docx-1-pie-10.1177_09544089231191905 for Experimental investigation on process parameters induced mechanical and microstructural properties for laser powder bed fusion additive manufacturing of SS316L by Meet Gor, Harsh Soni, Nishkarsh Srivastava, Amit Arora, Pankaj Sahlot, Ankit Oza and Anita Gehlot in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering</p
Tube equal channel angular extrusion as a novel severe plastic deformation technique for Mg-3Al-1Zn alloy and pure niobium
Get PDFSevere plastic deformation (SPD) processed ultrafine grained (UFG) materials are finding wide application in many industries because of their superior properties. Plenty of work has been done on the SPD processing of bar, plate, and rod forms of materials. Unfortunately, not much study has been conducted on improving the fabrication technique of tubular forms of materials. The conventional tube manufacturing processes involve either a seamless tube production method using high temperature extrusion which results in a large, non-uniform grain size or production of tubes that are seam welded after extrusion through a porthole or spider die. Welded regions are often the weak part of the tube and are prone to early failure.
An attractive substitute for conventional tube forming could be SPD processing that imparts high strength by reducing the grain size and not having a seam weld. To achieve improvement in the physical and mechanical properties of tubes, use of a novel SPD processing technique of tube equal channel angular extrusion (tECAE) is presented in this dissertation. Processing of magnesium (Mg) alloy and pure niobium (Nb) tubes were conducted in this study.
Mg alloys tubes are promising candidates to be used in biomedical industries for stent application, in transportation, and lightweight engineering application. Mg alloys are difficult to work with and possess anisotropic properties. The tECAE technique successfully processed Mg-3Al-1Zn alloy tubes at temperatures as low as 120 ��C. The role of starting texture on strength and deformation behavior of the Mg alloy was investigated by developing new processing routes. A viscoplastic self-consistent crystal plasticity model was used to supplement the experimental findings and improve the understanding of deformation modes in Mg alloy. Improved mechanical properties and unique textures were obtained after tECAE processing.
Pure Nb tubes were also tECAE processed. Nb tubes are important for particle accelerators to make superconducting radio frequency cavities. The effect of accumulated strain during tECAE processing on the grain size, texture, and post-processing recrystallization temperature was investigated.
This work shows that tECAE processing has the potential to reduce asymmetry in the mechanical properties and improve the strength and ductility by effective grain refinement
Cosmological N-body simulations: a challenge for scalable generative models: Tensorflow checkpoints
No full text<p><strong>Tensorflow checkpoints: Cosmological N-body simulations: a challenge for scalable generative models</strong></p>
<p>This corresponds to the Tensorflow checkpoints for the experiments in the paper <strong>Cosmological N-body simulations: a challenge for scalable generative models</strong> by Nathanaël Perraudin, Ankit Srivastava, Aurelien Lucchi, Tomasz Kacprzak, Thomas Hofmann, Alexandre Refregier, Adam Amara.</p>
<pre><code>@inproceedings{perraudin2019cosmological,
title = {Cosmological N-body simulations: a challenge for scalable generative models},
author = {Nathana\"el, Perraudin and Ankit, Srivastava and Kacprzak, Tomasz and Lucchi, Aurelien and Hofmann, Thomas and R{\'e}fr{\'e}gier, Alexandre},
year = {2019},
archivePrefix = {arXiv},
eprint = {1908.05519},
url = {https://arxiv.org/abs/1908.05519},
}
</code></pre>
<p>Please check the assotiated github page <a href="https://github.com/nperraud/3DcosmoGAN">https://github.com/nperraud/3DcosmoGAN</a> for additional information.</p>
<p>This corresponds to the Tensorflow checkpoints for the experiments in the paper<br>
**Cosmological N-body simulations: a challenge for scalable generative models** by<br>
Nathanaël Perraudin, Ankit Srivastava, Aurelien Lucchi, Tomasz Kacprzak, Thomas Hofmann, Alexandre Refregier, Adam Amara.</p>
<p>Please check the assotiated github page <a href="https://github.com/nperraud/3DcosmoGAN">https://github.com/nperraud/3DcosmoGAN</a> for additional information.</p>
Read counts at multiple attenuation levels as an object localization technique using passive RFID tags
No full textRadio Frequency Technologies (RFID) are experiencing rapid development and business, retail, manufacturing and healthcare are the major application areas benefiting from it. We describe and analyze an algorithm which helps in tracking of medical equipment and personnel and patients in a Trauma Bay. We adapt the Read Count algorithm to be used at multiple attenuation levels as a localization technique. The input parameter to the algorithm is the read count value which gives a measure of number of times the back-scattered radio frequencies from passive tags has been received by the antenna. Special attention has to be given to the placement of antennas to get the optimum result. The detection of multiple tags and human occlusion are two major concerns which we have tried to solve by suing multiple horizontal antennas along with 1 vertical antenna. We have discussed the results and analysis of such a configuration and accordingly given a conclusion. The problems associated with the configuration have also been discussed which can form part of future work.M.S.Includes bibliographical referencesby Ankit Sard
THD Analysis of Cascaded Multi level Inverters using different PWM Techniques
No full textMulti level inverters MLI are used in places or industries that require very high voltage and huge current. Multi level inverter topologies have various advantages over single level converters. These advantages are like rise in output voltage, reduction in the total harmonic distortion THD , reduction in electro magnetic interference EMI generation etc. Key attribute of a multi level inverter is the reduction in the voltage stress on each power device. This reduction is due to the use of several levels available on the DC bus. The dawn of multi level inverter topologies have created requirement of various pulse width modulation techniques. In this paper authors have used PWM technique, by which the total harmonic distortion of the multi level inverter is decreased. In this we have also controlled the speed of the induction motor by supplying the power through a multi level inverter. Shilpa Sambhi | Ankit Mittal | Aviral Srivastava | Ayan Mondal | Nitin Varshney "THD Analysis of Cascaded Multi-level Inverters using different PWM Techniques" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4 , June 2019, URL: https://www.ijtsrd.com/papers/ijtsrd23984.pd
Development of QSAR model for immunomodulatory activity of natural coumarinolignoids
No full textDharmendra K Yadav, Abha Meena, Ankit Srivastava, D Chanda, Feroz Khan, SK ChattopadhyayMetabolic and Structural Biology Department, Central Institute of Medicinal and Aromatic Plants, Council of Scientific and Industrial Research, PO-CIMAP, IndiaAbstract: Immunomodulation is the process of alteration in immune response due to foreign intrusion of molecules inside the body. Along with the available drugs, a large number of herbal drugs are promoted in traditional Indian treatments, for their immunomodulating activity. Natural coumarinolignoids isolated from the seeds of Cleome viscose have been recognized as having hepatoprotective action and have recently been tested preclinically for their immunomodulatory activity affecting both cell-mediated and humoral immune response. To explore the immunomodulatory compound from derivatives of coumarinolignoids, a quantitative structure activity relationship (QSAR) and molecular docking studies were performed. Theoretical results are in accord with the in vivo experimental data studied on Swiss albino mice. Immunostimulatory activity was predicted through QSAR model, developed by forward feed multiple linear regression method with leave-one-out approach. Relationship correlating measure of QSAR model was 99% (R2 = 0.99) and predictive accuracy was 96% (RCV2 = 0.96). QSAR studies indicate that dipole moment, steric energy, amide group count, lambda max (UV-visible), and molar refractivity correlates well with biological activity, while decrease in dipole moment, steric energy, and molar refractivity has negative correlation. Docking studies also showed strong binding affinity to immunomodulatory receptors.Keywords: coumarinolignoids, immunomodulation, docking, QSAR, regression mode
Multi-source Neural Automatic Post-Editing: FBK’s participation in the WMT 2017 APE shared task
Get PDFPrevious phrase-based approaches to Automatic Post-editing (APE) have shown that the dependency of MT errors from the source sentence can be exploited by jointly learning from source and target information. By integrating this notion in a neural approach to the problem, we present the multi-source neural machine translation (NMT) system submitted by FBK to the WMT 2017 APE shared task. Our system implements multi-source NMT in a weighted ensemble of 8 models. The n-best hypotheses produced by this ensemble are further re-ranked using features based on the edit distance between the original MT output and each APE hypothesis, as well as other statistical models(n-gram language model and operation sequence model). This solution resulted in the best system submission for this round of the APE shared task for both en-de and de-en language directions. For the former language direction, our primary sub-mission improves over the MT baseline up to -4.9 TER and +7.6 BLEU points. For the latter, where the higher quality of the original MT output reduces the room for improvement, the gains are lower but still significant (-0.25 TER and +0.3 BLEU)
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