2,875 research outputs found
Light-induced structural changes occur? in the transmembrane helices of the Natronobacterium pharaonis HtrII? transducer.
No full text
Cytoplasmic?membrane-proximal domain interaction of NpSRII-NpHtrII is important?for signal transduction in natronobacterium pharaonis.
No full text
Modification of Loop 1 Affects the Nucleotide Binding Properties of Myo1c, the Adaptation Motor in the Inner Ear
Full text linkMyo1c is one of eight members of the mammalian myosin I family of actin-associated molecular motors. In stereocilia of the hair cells in the inner ear, Myo1c presumably serves as the adaptation motor, which regulates the opening and closing of transduction channels. Although there is conservation of sequence and structure among all myosins in the N-terminal motor domain, which contains the nucleotide- and actin-binding sites, some differences include the length and composition of surface loops, including loop 1, which lies near the nucleotide-binding domain. To investigate the role of loop 1, we expressed in insect cells mutants of a truncated form of Myo1c, Myo1c1IQ, as well as chimeras of Myo1c1IQ with the analogous loop from other myosins. We found that replacement of the charged residues in loop 1 with alanines or the whole loop with a series of alanines did not alter the ATPase activity, transient kinetics properties, or Ca2+ sensitivity of Myo1c1IQ. Substitution of loop 1 with that of the corresponding region from tonic smooth muscle myosin II (Myo1c1IQ-tonic) or replacement with a single glycine (Myo1c1IQ-G) accelerated the release of ADP from A.M 2?3-fold in Ca2+, whereas substitution with loop 1 from phasic muscle myosin II (Myo1c1IQ-phasic) accelerated the release of ADP 35-fold. Motility assays with chimeras containing a single ?-helix, or SAH, domain showed that Myo1cSAH-tonic translocated actin in vitro twice as fast as Myo1cSAH-WT and 3-fold faster than Myo1cSAH-G. The studies show that changes induced in Myo1c via modification of loop 1 showed no resemblance to the behavior of the loop donor myosins or to the changes previously observed with similar Myo1b chimeras
Reflections of a Jewish, Lesbian Author
Full text linkIn this essay, Jewish lesbian author Leslea Newman speaks of the importance of finding one's own identity reflected in works of literature, citing examples of her own work, and recommending the writings of other Jewish lesbian authors of merit
Staging the life-world: Habermas and the recuperation of Austin speech act theory
No full textPT: J; CR: APEL KO, 1976, SPRACHPRAGMATIK PHIL AUSTIN JL, 1962, HOW TO DO THINGS WOR AUSTIN JL, 1970, PHILOS PAPERS CULLER J, 1982, DECONSTRUCTION DERRIDA J, 1977, GLYPH, V1 ECO U, 1992, UNDERSTANDING ORIGIN, P273 FISH S, 1987, TRACING LIT THEORY HABERMAS J, 1984, THEORY COMMUNICATIVE, V1 HABERMAS J, 1987, THEORY COMMUNICATIVE, V2 HABERMAS J, 1989, JURGEN HABERMAS SOC MARTINET A, 1962, FUNCTIONAL VIEW LANG, P24 QUINE WV, 1960, WORD OBJECT SEARLE JR, 1969, SPEECH ACTS SEARLE JR, 1977, GLYPH, V1 VANEEMEREN F, 1983, SPEECH ACTS ARGUMENT WARNOCK GJ, 1989, FL AUSTIN; NR: 16; TC: 0; J9: J THEOR SOC BEHAV; PG: 12; GA: KR147Source type: Electronic(1
MODEIN2 and Colby: computer codes for sediment transport computations
Full text linkNovember, 1976.CER76-77VMP-JL-DBS19
Tunneling microscopy of NbSe2 in air
No full textPT: J; CR: BANDO H, 1987, JPN J APPL PHYS PT 2, V26, L41 BINNIG G, 1982, PHYS REV LETT, V49, P57 BRYANT A, 1986, APPL PHYS LETT, V48, P832 FELDMAN JL, 1976, J PHYS CHEM SOLIDS, V37, P1141 FELDMAN JL, 1981, J PHYS CHEM SOLIDS, V42, P1029 JERICHO MH, 1980, PHYS REV B, V22, P4907 JERICHO MH, 1987, REV SCI INSTRUM, V58, P1349 MAMIN HJ, 1986, PHYS REV B, V34, P9015 PETHICA JB, 1986, IBM J RES DEV, V30, P455 RAO GVS, 1979, PHYSICS CHEM MATERIA, P99 SOLER JM, 1986, PHYS REV LETT, V57, P444 TERSOFF J, 1986, PHYS REV LETT, V57, P440 TOKUMOTO H, 1986, JPN J APPL PHYS, V25, L621; NR: 13; TC: 14; J9: J APPL PHYS; PG: 4; GA: L5219Source type: Electronic(1
Absence of xenotropic murine leukaemia virus-related virus in UK patients with chronic fatigue syndrome
Full text linkBackground: Detection of a retrovirus, xenotropic murine leukaemia virus-related virus (XMRV), has recently been reported in 67% of patients with chronic fatigue syndrome. We have studied a total of 170 samples from chronic fatigue syndrome patients from two UK cohorts and 395 controls for evidence of XMRV infection by looking either for the presence of viral nucleic acids using quantitative PCR (limit of detection <16 viral copies) or for the presence of serological responses using a virus neutralisation assay.
Results: We have not identified XMRV DNA in any samples by PCR (0/299). Some serum samples showed XMRV neutralising activity (26/565) but only one of these positive sera came from a CFS patient. Most of the positive sera were also able to neutralise MLV particles pseudotyped with envelope proteins from other viruses, including vesicular stomatitis virus, indicating significant cross-reactivity in serological responses. Four positive samples were specific for XMRV.
Conclusions: No association between XMRV infection and CFS was observed in the samples tested, either by PCR or serological methodologies. The non-specific neutralisation observed in multiple serum samples suggests that it is unlikely that these responses were elicited by XMRV and highlights the danger of over-estimating XMRV frequency based on serological assays. In spite of this, we believe that the detection of neutralising activity that did not inhibit VSV-G pseudotyped MLV in at least four human serum samples indicates that XMRV infection may occur in the general population, although with currently uncertain outcomes
- …
