113,996 research outputs found
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Fbxo45 binds SPRY motifs in the extracellular domain of N-cadherin and regulates neuron migration during brain development.
No full textSeveral events during normal development of the mammalian neocortex depend on N-cadherin, including the radial migration of immature projection neurons into the cortical plate. Remarkably, radial migration requires the N-cadherin extracellular domain but not N-cadherin-dependent homophilic cell-cell adhesion, suggesting that other N-cadherin-binding proteins may be involved. We used proximity ligation and affinity purification proteomics to identify N-cadherin-binding proteins. Both screens detected MycBP2 and SPRY-domain protein Fbxo45, two components of an intracellular E3 ubiquitin ligase. Fbxo45 appears to be secreted by a non-classical mechanism, not involving a signal peptide and not requiring endoplasmic reticulum to Golgi transport. Fbxo45 binding requires N-cadherin SPRY motifs that are not involved in cell-cell adhesion. SPRY-mutant N-cadherin does not support radial migration in vivo. Radial migration was similarly inhibited when Fbxo45 expression was suppressed. The results suggest that projection neuron migration requires both Fbxo45 and binding of Fbxo45 or another protein to SPRY motifs in the extracellular domain of N-cadherin
Kustverdediging na 1990 (Kustnota 1990): Technisch rapport 18: Berekeningsresultaten beleidsalternatieven
No full textVanuit een beschrijving van beleidsanalyse in algemene zin en de gebruikelijke terminologie en definiering ervan, wordt toegewerkt naar een specificatie van de voor de discussienota 'Kustverdediging na 1990' gehanteerde beleidsanalytische aanpak. Hierin wordt de doelstelling van de studie uitgewerkt, wordt de uitgangssituatie gespecificeerd en worden de randvoorwaarden beschreven. De gewenste ontwikkelingen ten aanzien van de kustverdediging en de relatie met de maatschappelijke funkties in het kustgebied worden vastgelegd in strategieen. Wanneer ook de maatregelen die deze strategieen konkreet maken worden ingevuld leidt dit tot (beleids) alternatieven. Tevens wordt aangegeven welke ontwikkelingen de inspanningen die voor de kustverdediging de komende decennia nodig zijn, in hoofdzaak bepalen. Deze worden uitgedrukt in scenario's en betreffen vooral de hydrometeorologische randvoorwaarden (zeespiegel, klimaat) en de morfologische ontwikkelingen (gemiddeld, ongunstig). Tot slot worden de criteria beschreven op basis waarvan de ontwikkelingen bij diverse beleidskeuzes kunnen worden vergeleken. Het betreft kosten, verliezen aan duinareaal, e.d. Ten behoeve van de integrale analyse is het systeemanalytische model KUSTBEL (TR-17 ) ontwikkeld waarmee op relatief eenvoudige wijze voor de verschillende beleidsalternatieven de resulterende kustontwikkeling en de effecten daarvan op veiligheid en functies kunnen worden geanalyseerd en vergeleken. De feitelijke uitvoering van de beleidsanalyse richt zich op de analyse van beleidsalternatieven aan de hand van concrete rekengevallen, die in KUSTBEL worden ingevoerd (uitgezonderd de strategie Zeewaarts). Als onderdeel van de analyse is tevens een gevoeligheidsanalyse uitgevoerd om de invloed te kunnen inschatten van de onzekerheden die samenhangen met de beschikbare kennis en informatie.Kustnot
Molecular contest between potato and the potato cyst nematode Globodera pallida: modulation of Gpa2-mediated resistance
No full textGpa2 recognition specificity Among all the multicellular animals, nematodes are the most numerous. In soil, a high variety of free living nematodes feeding on bacteria can be found as well as species that parasitize insects, animals or plants. The potato cyst nematode (PCN) Globodera pallida is an important pest of cultivated potato. Upon infection of the roots, the nematode induces a feeding cell complex or so-called syncytium, on which the immobilized nematode fully depends for its development and reproduction. Due to the sophisticated feeding manner and ability to survive for a long time in the absence of a host plant, the best way to control these soil-born pathogens is the exploitation host resistance. Natural resistance to nematodes is based on single dominant resistance genes (R) or quantitative trait loci (QTL). Several nematode resistance genes have been identified and mapped. This includes the potato gene Gpa2 (Van der Vossen et al., 2000) that confers resistance against the population D383 of G. pallida. The Gpa2 gene is highly homologous to Rx1, which confers resistance against potato virus X (Bendahmane et al., 1999). Both genes encode a protein with a nucleotide-binding leucinerich repeat (NB-LRR) domains and a short coiled-coil domain at the N-terminus, which are in 88% identical at the amino acid level. The vast majority of the differences between Gpa2 and Rx1 is found in the predicted solvent exposed regions of the LRR domain. In chapter 2, we have shown that the LRR domain is essential for the recognition specificities of Gpa2 and Rx1, whereas the CC-NBS domains can be exchanged without affecting the specificity. In chapter 5, we have used a series of chimeric constructs in which segments of the Gpa2 LRR were replaced by the corresponding segments from Rx1. These constructs allowed us to narrow down the region required for nematode recognition to a stretch of residues between 808 and 912 amino acid residues in Gpa2, including 10 amino acids that differ between Gpa2 and Rx1. Furthermore, a computer-aided 3D model of the LRR domain is presented in which 7 of the Gpa2 specific amino acid residues map in a cluster onto the concave surface of the horseshoe-like structure of the LRR domain. Gpa2-mediated nematode resistance The research described in chapter 3 aimed to understand the mechanisms underlying Gpa2- mediated resistance to the potato cyst nematode G. pallida. The extreme resistance response conferred by the close homologue Rx1 results in the blocking of the potato virus X (PVX) at the infection sites and hence, the prevention of systemic spreading throughout the plant. Surprisingly, an entirely different defense mechanism was observed for resistant potato plants infected with juveniles of the avirulent Globodera pallida population D383. In susceptible plants, both the virulent population Rookmaker and the avirulent population D383 formed normal developing syncytia and nematodes were able to complete their life cycle as described in previous studies. Infection of resistant plants with the avirulent population showed no differences between susceptible and resistant potato plants in the early stages of G.pallida parasitism (root entering, migration, syncytium initiation). Syncytium induction took place in parenchyma cells, but rarely in other tissues. In samples collected 7 days later, however, the first necrotic cells in the surrounding of the syncytium were noticed including symptoms of degradation in the ultra structure of the syncytium itself in case of resistant plants infected with avirulent nematodes. Samples collected 10 days post infection had already a layer of necrotic cells, which separates the syncytium from the vascular bundle. At 14 days post infection, it was observed that the parenchyma cells not incorporated directly in the syncytia started to divide fast. Groups of hyperplastic cells surrounding the degrading syncytium resulted in pushing it away to the outer part of the root. This unique phenomenon, which was not observed before, can be part of the Gpa2-mediated defense response or a secondary reaction to the presence of necrotic, dead cells and a way to exclude them from the healthy conductive tissue of the root. Transcriptional regulation of the Gpa2 promoter To look in more details into the transcriptional regulation and expression of Gpa2, the native promoter was fused to the reporter gene GUS and this construct was introduced into susceptible potato. In chapter 3, the activity of the Gpa2 promoter was observed and shown to be restricted to the vascular system and the root tips in uninfected plants. Roots were challenged with G.pallida and the localization of the GUS expression was observed at the infection sites at different parasitic stages. During infection with virulent nematodes - but not the avirulent ones - this activity seems to be down regulated in vicinity of the syncytium. Such a local inhibition of Gpa2 promoter activity is in line with observations made on resistant roots when necrotic cells were only present around the feeding cell complex, distantly from the feeding nematode. The effector protein RBP-1 elicits a Gpa2 dependent HR Recently, a RBP-1protein with strong similarity to the SPRY domain of the Ran-binding protein RanBPM in juveniles of G. pallida was identified as a putative Gpa2 elicitor. Transient expression of RBP-1 in N. benthamiana leaves elicits a Gpa2-dependent cell death typical for the R-gene associated hypersensitive response (HR). Total RNA isolated from two populations of G.pallida, D383 (avr to Gpa2) and Rookmaker (vir to Gpa2) was converted into cDNA and screened for the presence of RBP-1s. This screening allowed the identification of in total 10 classes of closely related homologs of RBP-1. All identified classes were tested for their ability to elicit the Gpa2-dependent HR in an agroinfiltration assay. The capacity to induce an Gpa2-dependent HR was shown to correlate with a single amino acid substitution in RBP-1. No response was observed for two classes, which were obtained from the virulent population (RBP-1ROOK2, RBP-1ROOK4). For the other homologous RBP-1 classes – both deriving from the virulent and avirulent population - the response was ranging from a mild to a strong and fast HR. Both in-active RBP-1 variants have a serine substitution at position 166 (S166P) within the SPRY domain. When this residue was projected on a computer aided 3D model of RBP, we noticed that this amino acid is in a loop extending from the protein core. Replacing the proline into a serine is predicted to change the shape of the loop and hence, to affect the potential surface for protein-protein interactions. Non-eliciting RBP-1 variants suppress RBP-induced Gpa2 activation It was shown that the non-eliciting variants (RBP-1ROOK2 and RBP-1ROOK4) can suppress the activation of a Gpa2-mediated HR by the eliciting RBP-1 variants. This effect was specific for the Gpa2-mediated HR, and not observed with a Rx1-induced HR. As autoactive mutants of Gpa2 and Rx1-mediated cell death are not blocked by the inactive variants of RBP-1, the mechanism of suppression or inhibition likely operates on a functional Gpa2 protein, instead of downstream Gpa2-activated signaling pathways. Further research is required to resolve the mechanism underlying the possible competitive interactions of the active and the inactive RBP-1 variants on the Gpa2-mediated HR. Essentially, two possible models that could explain this phenomenon. First, the inactive variants could physically out compete the active RBP-1s. The binding target of active and inactive variants of RBP-1 variants could be directly in the Gpa2 protein or in the virulence target monitored by Gpa2. Alternatively, the inactive variants of RBP-1 may intercept active RBP-1 variants by forming an inactive heterodimer complex rendering it essentially undetectable for the Gpa2 protein. <br/
Kustverdediging na 1990 (Kustnota 1990): Technisch rapport 17: Systeemanalytisch model
No full textHet doel van de discussienota 'Kustverdediging na 1990' is het voorbereiden van beleidskeuzen met betrekking tot de landelijke aanpak van de kustverdediging. Een groot aantal afwegingen en keuzen speelt hierbij een rol. Het is noodzakelijk het gehele scala aan mogelijkheden te vertalen in een aantal goed gedefinieerde beleidsalternatieven. Deze moeten systematisch worden onderzocht om tot een vergelijkende beoordeling te kunnen komen. Gekozen is voor de ontwikkeling van een systeemanalytisch model waarmee beleidsalternatieven doorgerekend kunnen worden. Dit model heet KUSTBEL. Het systeemanalytische model maakt het mogelijk een analyse van het kustsysteem uit te voeren. Hierbij wordt een voorspelde kustontwlkkeling getoetst aan maatschappelijke eisen die zijn gespecificeerd in de te onderzoeken beleidsalternatieven. Het model voert de berekeningen uit en bepaalt de gevolgen van een beleidsalternatief in termen van kosten en baten.Kustnot
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No full textDistinguished Alumni Award Program author. LTG David K. Heebner, USA (Ret) (Presented 13 Aug 14)NANAhttp://archive.org/details/adevelopmentndco109451773
Kustverdediging na 1990 (Kustnota 1990): Technisch rapport 15: Monitoring kustgedrag
No full textIn 1990 is de sterkte van het systeem van waterkeringen van ons land tegen de bedreiging door de zee op zgn. delta-veilig niveau gebracht. Deze veiligheid zal echter onder druk komen te staan door de huidige morfologische ontwikkelingen van onze kust, waarbij zeespiegelrijzing een belangrijke rol speelt. Om het verkregen veiligheidsniveau te kunnen handhaven en het kustgedrag beter te leren begrijpen en te voorspellen, is het noodzakelijk ons kustsysteem en de factoren die het beïnvloeden nauwlettend in de gaten te houden. Doel van deze studie is na te gaan hoe we dat in het licht van de huidige kennis het beste kunnen doen. Het huidige meetprogramma wordt daartoe beschreven en geevalueerd. Waar nodig worden aanpassingen voorgesteld. Tevens wordt een blik op de verdere toekomst geworpen in het hoofdstuk over remote sensing technieken.Kustnot
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No full textThis thesis is a continuation of the study work done by Moedjiono (M.S. thesis, Naval Postgraduate School, 1982) concerning personnel database in the Indonesian Navy. It discusses the current database security and the concept of Multics (Multiplexed Information and Computing System) to propose a personnel database security model in the Indonesian Navy. (Author)NANAhttp://archive.org/details/thepplicationofs109451964
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No full textNAU.S. Marine Corps (U.S.M.C.) author; U.S. Navy (U.S.N.) author.http://archive.org/details/frequencyrespons109452656
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