32,196 research outputs found
Anions and the bilayer. Structural and mechanistic studies towards synthetic anion carriers for therapeutic applications
No full textSynthetic transmembrane anion transporters have attracted a great deal of interest due to their potential as therapeutic agents for the treatment of channelopathies such as cystic fibrosis or in the treatment of cancer. Despite a great deal of progress in the field over recent years, there are currently several hurdles that need to be overcome before anionophores become genuine therapeutic candidates. These include knowledge of how carriers behave in more cell-like bilayer systems, what molecular properties govern the rate determining step of the transport process and issues with solubility and deliverability. This thesis aims to explore some of these areas to help overcome some of these future barriers.The effect of lipid environment on the transport ability of a series of alkyl-substituted thioureas was investigated. The series covered a wide range of lipophilicity, to determine whether the optimum lipophilic range varied depending on the composition of the bilayer. Despite the different lipids appearing to modulate the overall transport rate, the relative order of the transporter efficacy appeared unaffected.Data is also presented demonstrating the use of dynamic covalent chemistry to generate an active transporter molecule in situ within the membrane. The compound formed by DCC was observed by measuring its transport response from vesicle experiments after the addition of two precursors (which do not facilitate transport alone) to the membrane. Comparison of the transport ability of the pre-formed compound and the rate of the DCC reaction measured by NMR spectroscopy gave insight into the balance required between these two factors in the design of these compounds.Finally, the effect of the fluorination of alkyl chains in tripodal tris-thiourea receptors was explored. Vesicle assays with and without accompanying protonophores to couple to the transport process were carried out to determine the mechanism of the transport process and discover whether fluorination had any effect on the selectivity of the transporter molecules. The activity of the compounds in FRT-YFP cell assays was also determined and comparison with the vesicle data gave insights into the vesicle tests required to accurately predict the transporters’ efficacy in cell epithelia
Michael Rodriguez interviews fiction writer Michael Kimball
No full textAuthor Michael Kimball talks about moving away from Michigan to become a successful writer, his education, the fiction reading series he has started in Baltimore, the life-story-on-postcard project, and his book "Dear everybody." Kimball is interviewed by Michigan State University Librarian Michael Rodriguez for the Michigan State University Libraries' Michigan Writers Series
Michael Rodriguez interviews author Paul Clemens
No full textAuthor Paul Clemens talks about his book "Made in Detroit," the genre of memoir, and writing about race. Clemens is interviewed by Michigan State University Librarian Michael Rodriguez for the MSU Libraries' Michigan Writers Series. Held in the MSU Main Library
Michael Rodriguez interviews author Tom Springer
No full textAuthor Tom Springer is interviewed about his writing career and his newest book "Looking for hickories". Springer talks about his career following after earning an Environmental Journalism degree from Michigan State University. He calls his genre "creative non-fiction" and explains how he weaves his memories into his books about life in rural and wild Michigan. Part of the Michigan State University Libraries' Michigan Writers Series. Springer is interviewed by Librarian Michael Rodriguez
Michael Rodriguez interviews author Gary Gildner
No full textAuthor Gary Gildner explains why he left his tenured teaching position to move to Idaho to became a full-time writer of poetry. Gildner talks about donating his personal papers to Michigan State University Libraries' Special Collections, his writing style and how he approaches writing. Gildner is interviewed by MSU Librarian Michael Rodriguez for the MSU Libraries' Michigan Writer Series. Held at the MSU Main Library
Gold standard of UK degrees is lost in translation
No full textInflated marks, overworked staff and politically compromised courses are the price of exploiting offshore UK registered students, says Michael Day
Michael Rodriguez interviews historian and author Keith Widder
No full textHistorian and author Keith Widder talks about his move to Michigan from Wisconsin, his career as Curator of History for the Mackinac Island State Park Commission, his research interests, his book "Michigan Agricultural College", and his current projects. Widder is interviewed by Michigan State University Librarian Michael Rodriguez for the MSU Libraries' Michigan Writers Series. Held in the MSU Main Library
Anion transport across varying lipid membranes – the effect of lipophilicity
No full textThe anion transport properties of a range of alkyl-substituted phenylthioureas were tested in vesicles of different lipid composition. Although changes in the bilayer affected the rate of transport for all compounds in the series, the ‘ideal’ log P for peak activity did not change depending on the composition of the bilayers tested
Dr. Michael Janis, Morehouse College, August 2011, August 2011
No full textThis video is a conversation with Dr. Michael Janis. Dr. Janis talks about his book, "Africa After Modernism: Transitions in Literature, Media and Philosophy". Yolanda Gilmore-Bivins, AUC Woodruff Library, is the interviewer
A tripodal tris-selenourea anion transporter matches the activity of its thio- analogue but shows distinct selectivity<sup>*</sup>
No full textWe report the synthesis of a tripodal tris-selenourea transporter scaffold. The Cl− and (Formula presented.) transport activity of the compound has been compared extensively with the analogous oxo- and thiourea compounds. We found that the selenourea demonstrates remarkably similar transport efficacy and mechanistic properties to the equivalent thiourea, but demonstrates flipped selectivity for Cl− over (Formula presented.).</p
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