261 research outputs found

    Trichoderma‐Derived Pentapeptides from the Infected Nest Mycobiome of the Subterranean Termite Coptotermes testaceus

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    Termites live in a dynamic environment where colony health is strongly influenced by surrounding microbes. However, little is known about the mycobiomes of lower termites and their nests, and how these change in response to disease. Here we compared the individual and nest mycobiomes of a healthy subterranean termite colony (Coptotermes testaceus) to one infected and ultimately eradicated by a fungal pathogen. We identified Trichoderma species in the materials of both nests, but they were also abundant in the infected termites. Methanolic extracts of Trichoderma sp. FHG000531, isolated from the infected nest, were screened for secondary metabolites by UHPLC‐HR MS/MS‐guided molecular networking. We identified many bioactive compounds with potential roles in the eradication of the infected colony, as well as a cluster of six unknown peptides. The novel peptide FE011 was isolated and characterized by NMR spectroscopy. The function of this novel peptide family as well as the role of Trichoderma species in dying termite colonies therefore requires further investigation

    Daniel 9 as part of an apocalyptic book?

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    Daniel 9 relates how Daniel studies the Hebrew Holy Scriptures and finds the prophecy of Jeremiah that Jerusalem will lie desolate for seventy years. He reacts by devoting himself to prayer and fasting in order to remind God of this promise of restoring his people. The better part of the chapter is dedicated to the contents of his prayer. During the prayer, the man, Gabriel, appears with the intent to give Daniel an understanding of the meaning of the seventy years, which is the measure of the punishment of Israel�s transgression and sin and which will end with eternal righteousness, when the Holy of Holies will be anointed. The Book of Daniel consists of two sections: the tales of the first six chapters and the visions of the last six chapters. This article asked the question: what role does Daniel 9 play as a part of the apocalyptic section of the book? Is Daniel�s prayer and Gabriel�s revelation apocalyptically conditioned? Why did the author or compiler include it in the book and, especially, in the second, apocalyptic section of the book? The conclusion of this article was that Daniel 9 was placed intentionally by the compiler in the latter half of the book because of the revelation about the seventy weeks, which is in line with the last three chapters� indication of the end times and Israel�s elevation to become the ruler of the earth. Several arguments were formulated to support this conclusion.</span

    NFDMLab: Simulating nonlinear frequency division multiplexing in Python

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    Fiber-optic transmission based on nonlinear frequency division multiplexing (NFDM) has received much attention in recent years. We introduce NFDMLab, an open source software package for simulating NFDM transmissions written in the Python language.Accepted Author ManuscriptTeam Sander Wahl

    Transferability of Simple Sequence Repeat (SSR) Markers Developed in Litchi chinensis to Blighia sapida (Sapindaceae)

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    # The Author(s) 2009. This article is published with open access at Springerlink.com Abstract Ackee (Blighia sapida, Sapindaceae) is a multi-purpose fruit tree species of high economic importance, native to the Guinean forests of West Africa, and belongs to the same family as that of lychee (Litchi chinensis). In this study, a set of 12 primer pairs for simple sequence repeats (SSRs) previously developed for lychee has been evaluated for polymorphism in 16 ackee trees from different populations. Seven primer pairs have been found to be transferable, and four have revealed polymorphisms. How-ever, the average number of alleles per locus has dropped from 4.9 for lychee to 3.7 for ackee. Characterization of the four polymorphic markers in 279 individuals belonging to14 different ackee populations from Benin has revealed that the numbers of alleles per locus range from two to 14 with a mean number of 5.8. The observed and expected heterozygosities range between 0.020 to 0.359 and 0.020 to 0.396, respectively

    A power saving protocol for impedance spectroscopy

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    Identification of a novel aminopolycarboxylic acid siderophore gene cluster encoding the biosynthesis of ethylenediaminesuccinic acid hydroxyarginine (EDHA)

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    Spohn M, Edenhart S, Alanjary M, et al. Identification of a novel aminopolycarboxylic acid siderophore gene cluster encoding the biosynthesis of ethylenediaminesuccinic acid hydroxyarginine (EDHA). METALLOMICS. 2018;10(5):722-734.The mechanism of siderophore-mediated iron supply enhances fitness and survivability of microorganisms under iron limited growth conditions. One class of naturally occurring ionophores is the small aminopolycarboxylic acids (APCAs). Although they are structurally related to the most famous anthropogenic chelating agent, ethylenediaminetetraacetate (EDTA), they have been largely neglected by the scientific community. Here, we demonstrate the detection of APCA gene clusters by a computational screening of a nucleotide database. This genome mining approach enabled the discovery of a yet unknown APCA gene cluster in well-described actinobacterial strains, either known for their potential to produce valuable secondary metabolites (Streptomyces avermitilis) or for their pathogenic lifestyle (Streptomyces scabies, Corynebacterium pseudotuberculosis, Corynebacterium ulcerans and Nocardia brasiliensis). The herein identified gene cluster was shown to encode the biosynthesis of APCA, ethylenediaminesuccinic acid hydroxyarginine (EDHA). Detailed and comparatively performed production and transcriptional profiling of EDHA and its biosynthesis genes showed strict iron-responsive biosynthesis

    Radar echoes of individual wind turbines measured in L, S and C band

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    In the project WERAN it is also aimed to determine the interaction of wind turbines (WT) with radar signals. The evaluate the strength of single a single WT reflecting radar transmissions, measurement campaigns with multicopters (UAS) have been carried out at various radar sites in the L (Air Defense), S (AD and ATC) and C band (Precipitation radar). The presentation focusses on radar echoes received at different UAS flight altitudes and distances to the scatterers. It is useful to analyse the radar return both in the time and frequency domain to detect static and time variant signal components. Some results will be shown and discussed. In the follow-up project WERAN plus there are some additional airborne vehicles in use to carry the measurement equipment. Some ideas of what can be expected from measurements at higher altitudes and moving at horizontal speed will be shared
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