1,721,163 research outputs found
Dataset for: High-Speed Single-Cell Dielectric Spectroscopy
No full textDataset for: Spencer, Daniel and Morgan, Hywel. (2020) High-Speed Single-Cell Dielectric Spectroscopy. ACS Sensors. DOI: https://doi.org/10.1021/acssensors.9b02119</span
Spencer, Daniel-Residence P.1
No full textSpencer, Daniel -- Residence. Located on northwest corner of Third South and State Streets
Dataset in support of the journal article: 'Electrical Broth Micro-Dilution for Rapid Antibiotic Resistance Testing'
No full textData is plain text in four sheets in a single excel spreadsheet. Description of methods used for collection/generation of data and for processing the data see: https://pubs.acs.org/doi/10.1021/acssensors.2c02166
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Advanced microfluidic impedance cytometry for point of care analysis
No full textMicrofluidic impedance cytometry is the dielectric characterisation of single particles flowing through a microfluidic channel. Microfluidic impedance cytometry offers a novel solution to counting and discrimination of different particles and cells. One application is discrimination of different blood cell types in a low cost Point of Care device. However, the accuracy of currently available systems is poor and inadequate for clinical use. A full numerical model, developed in this research, demonstrated that the main source of measurement inaccuracy relates to the position of the particle in the micro-channel. Particles travelling at the top or bottom of the channel close to the electrodes were found to have a higher impedance signal compared with particles travelling through the centre of the channel. Counter-intuitively, the model also showed that although the electrode geometry is symmetrical, the variation in signal is asymmetric about the vertical position of the particle within the channel. A new electrode geometry was developed to minimise particle positional dependence. When combined with new signal processing techniques which were also developed, this led to marked improvements in measurement accuracy. For example, the coefficient of variation of monodisperse beads were half the manufacturer's data.Applications of the improved device were investigated by analysing a range of different cell types in blood. Measurements of the distribution width of red blood cells were successful and found to be within the known clinical range. It was also shown that combined with pre-enrichment techniques, the device successfully detected clinically relevant concentrations of individual tumour cells in a background of 106 times more white blood cells. In order to use microfluidic impedance cytometry for diagnostic blood counting applications, lysing of the more numerous red blood cells is required. Lysing methods suitable for implementing in a Point of Care device were evaluated and a stirred mixing system was developed. In summary, this thesis describes a new, high accuracy microfluidic impedance cytometer which has the potential to be integrated into a miniature blood counting device for Point of Care analysis
Data for 'High accuracy particle analysis using sheathless microfluidic impedance cytometry'
No full textData in matlab format for the article D. Spencer, F.
Caselli, P. Bisegna and H. Morgan, Lab Chip, 2016, DOI: 10.1039/C6LC00339G</span
High-speed single-cell dielectric spectroscopy
No full textSingle-cell impedance cytometry is a label-free analysis technique that is now widely used to measure the electrical properties of a cell and to differentiate different subpopulations. Current techniques are limited to measuring the impedance of a single cell at one or two simultaneous frequencies. Also, there are no methods that extrapolate the intrinsic electrical properties of single cells. We demonstrate a new approach that uses multifrequency impedance measurements to determine the complete intrinsic electrical properties of thousands of single cells at high throughput. The applicability of the method is demonstrated by measuring the properties of red blood cells and red cell ghosts, deriving the unique values of conductivity and permittivity of the membrane and cytoplasm for each individual cell
The development of gunpowder weapons in Late Medieval England
No full textThe present thesis is a study of the development of gunpowder weapons in Late Medieval England. This was a new technology that had reached Western Europe by the early fourteenth century, which had first supplemented and later supplanted traditional forms of artillery. The development of early firearms has long been recognised as significant by historians and has been identified as a key part of the military revolution hypothesis. As a result of this, gunpowder weapons are often discussed in general works on English military history but there is at the moment no satisfactory study on its long-term development in England. The aim of the present study is to rectify this gap in the literature by carrying out a thorough examination and comparison of the extensive surviving financial records for the English Crown and towns for the period covering the reigns of Edward III to Henry VII. This information will be analysed to determine how the use of guns on military campaigns, in towns, royal fortifications and on ships changed over time, as well as to assess what factors influenced the development of gunpowder weapons and to see if these changes constituted a military revolution. As a result of this research, it is now possible to establish a comprehensive narrative of how English gunpowder weapons developed throughout this critical period in the history of the technology
Raw Datasets for PhD thesis entitled Coastal-Estuarine Microbiology Explored: Community Dynamics, Biogeochemical Processes and Cell Specific Targeting of Nitrogen Cyclers
No full textThese datasets contain raw data for the PhD thesis entitled "Coastal-Estuarine Microbiology Explored: Community Dynamics, Biogeochemical Processes and Cell Specific Targeting of Nitrogen Cyclers", authored by Spencer Long.
Datasets are as follows:
- tamar_nutrients_master.csv contains raw nutrient data from the Tamar estuary, UK, for dates sampled in September 2019, March 2020, September 2021 and May 2022.
- L4_nutrients_master.csv contains raw nutrient data from Western Channel Observatory station L4, for dates sampled in September 2019, March 2020 and May 2022.
- nitrifier_CARD_stats.csv contains catalysed deposition fluorescence in situ hybridisation (CARD-FISH) obtained raw data for ammonia oxidising microbial communities within thew Tamar estuary. Data relates to samples taken in September 2019, March 2020, September 2021 and May 2022.
- nitrification_rate.csv contains calculated ammonia oxidation rate raw data for the Tamar estaury, relating to samples taken in September 2021 and May 2022.
- plate_reader_master.csv contains raw data obtained from all plate reader experiments relating to the chapter "Assessing the Applicability of DAF-FM Diacetate for the Specific Detection of Nitric Oxide Cycling Microorganisms"
- fluor_spreadsheet_final.csv contains raw data obtained from all epifluorescence microscopy experiments relating to the chapter "Assessing the Applicability of DAF-FM Diacetate for the Specific Detection of Nitric Oxide Cycling Microorganisms"
- readme.txt contains further data information
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