42 research outputs found
The French touch: feedback, challenges and prospects of vaccination against high pathogenicity influenza viruses in duck farming.
No full textInternational audienc
The French touch: feedback, challenges and prospects of vaccination against high pathogenicity influenza viruses in duck farming.
No full textInternational audienc
Study of factors modulating the emergence of high pathogenicity avian influenza viruses from a low pathogenicity precursor
No full textInternational audienc
Truncation of the NS1 Protein Converts a Low Pathogenic Avian Influenza Virus into a Strong Interferon Inducer in Duck Cells
No full textInternational audienc
Avian influenza viruses and ducks: investigating host-virus dialogue
No full textInternational audienc
Avian influenza viruses and ducks: investigating host-virus dialogue
No full textÉvénement(s) lié(s) : - 2. Sino-french meeting on infectious diseases; Wuhan (CHN) - (2011-10-19 - 2011-10-21)International audienc
Avian influenza viruses and ducks: investigating host-virus dialogue
No full textÉvénement(s) lié(s) : - 2. Sino-french meeting on infectious diseases; Wuhan (CHN) - (2011-10-19 - 2011-10-21)International audienc
Neuraminidase stalk deletion and evolution during field transmission of influenza A virus (H6N1) from ducks to turkeys
No full textInternational audienc
Species-Specific Contribution of the Four C-Terminal Amino Acids of Influenza A Virus NS1 Protein to Virulence
No full textLarge-scale sequence analyses of influenza viruses revealed that nonstructural 1 (NS1) proteins from avian influenza viruses have a conserved C-terminal ESEV amino acid motif, while NS1 proteins from typical human influenza viruses have a C-terminal RSKV motif. To test the influence of the C-terminal domains of NS1 on the virulence of an avian influenza virus, we generated a wild-type H7N1 virus with an ESEV motif and a mutant virus with an NS1 protein containing a C-terminal RSKV motif by reverse genetics. We compared the
phenotypes of these viruses in vitro in human, mouse, and duck cells as well as in vivo in mice and ducks. In human cells, the human C-terminal RSKV domain increased virus replication. In contrast, the avian Cterminal ESEV motif of NS1 increased virulence in mice. We linked this increase in pathogenicity in mice to an increase in virus replication and to a more severe lung inflammation associated with a higher level of production of type I interferons. Interestingly, the human C-terminal RSKV motif of NS1 increased viral replication in ducks. H7N1 virus with a C-terminal RSKV motif replicated to higher levels in ducks and
induced higher levels of Mx, a type I interferon-stimulated gene. Thus, we identify the C-terminal domain of NS1 as a species-specific virulence domain
Author response: Ubiquitination-dependent control of sexual differentiation in fission yeast
No full text
