62,558 research outputs found
The pstSCAB operon for phosphate uptake is regulated by the global regulator GlxR in Corynebacterium glutamicum
No full textPanhorst M, Sorger-Herrmann U, Wendisch VF. The pstSCAB operon for phosphate uptake is regulated by the global regulator GlxR in Corynebacterium glutamicum. Journal of Biotechnology. 2011;154(2-3):149-155
Phosphate starvation-inducible gene ushA encodes a 5 ' nucleotidase required for growth of Corynebacterium glutamicum on media with nucleotides as the phosphorus source
No full textRittmann D, Sorger-Herrmann U, Wendisch VF. Phosphate starvation-inducible gene ushA encodes a 5 ' nucleotidase required for growth of Corynebacterium glutamicum on media with nucleotides as the phosphorus source. Applied and Environmental Microbiology. 2005;71(8):4339-4344.Phosphorus is an essential component of macromolecules, like DNA, and central metabolic intermediates, such as sugar phosphates, and bacteria possess enzymes and control mechanisms that provide an optimal supply of phosphorus from the environment. UDP-sugar hydrolases and 5' nucleotidases may play roles in signal transduction, as they do in mammals, in nucleotide salvage, as demonstrated for UshA of Escherichia coli, or in phosphorus metabolism. The Corynebacterium glutamicum gene ushA was found to encode a secreted enzyme which is active as a 5' nucleotidase and a UDP-sugar hydrolase. This enzyme was synthesized and secreted into the medium when C. glutamicum was starved for inorganic phosphate. UshA was required for growth of C. glutamicum on AMP and UDP-glucose as sole sources of phosphorus. Thus, in contrast to UshA from E. coli, C. glutamicum UshA is an important component of the phosphate starvation response of this species and is necessary to access nucleotides and related compounds as sources of phosphorus
Albert Herrmann. Das Land der Seide und Tibet im Lichte der Antike. (Quellen u. forkundle zur Geschichte der Geographie u. hgg. v. Albert Herrmann, Band I)
No full textDept. G.-G. Albert Herrmann. Das Land der Seide und Tibet im Lichte der Antike. (Quellen u. forkundle zur Geschichte der Geographie u. hgg. v. Albert Herrmann, Band I). In: L'antiquité classique, Tome 8, fasc. 1, 1939. pp. 299-300
Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB
No full textSorger-Herrmann U, Taniguchi H, Wendisch VF. Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB. BMC Microbiology. 2015;15(1): 113.Background
The pstSCAB operon of Corynebacterium glutamicum, which encodes an ABC transport system for uptake of phosphate (Pi), is induced during the Pi starvation response. The two-component regulatory system PhoRS is involved in this response, but partial Pi starvation induction of pstSCAB in a ΔphoRS mutant indicated the involvement of additional regulator(s). Regulation of pstSCAB also involves the global transcriptional regulator GlxR.
Results
DNA affinity chromatography identified the regulator of acetate metabolism RamB as a protein binding to pstS promoter DNA in vitro. Gel mobility shift assays and mutational analysis of the pstS promoter region revealed that RamB binds to two sites localized at positions −74 to −88 and −9 to +2 with respect to the transcriptional start site of pstSCAB. Reporter gene studies supported the in vivo relevance of both binding sites for activation of pstSCAB by RamB. DNA microarray analysis revealed that expression of many Pi starvation genes reached higher levels during the Pi starvation response on minimal medium with glucose as sole carbon source than in Pi starved acetate-grown C. glutamicum cells.
Conclusions
In C. glutamicum, RamB is involved in expression control of pstSCAB operon. Thus, transcriptional regulation of pstSCAB is complex involving activation by the phosphate-responsive two-component regulatory system PhoSR and the regulators of carbon metabolism GlxR and RamB
Odontomachus schoedli Sorger & Zettel, 2011, sp.n.
No full textOdontomachus schoedli sp.n. (Figs. 4, 10, 16, 47) Etymology: This species is named for our late colleague Stefan Schödl, former curator for Hymenoptera at the Natural History Museum Vienna, who collected a major part of the type series. Type material: Holotype worker (UPLB): Luzon: Mountain Province: south of Sagada, Bagnen, slopes of Mt. Polis, 1600 m, 26.II.1999, leg. S. Schödl (23). Paratypes(24 workers (BMNH, CZW, NHMW, UPLB): same locality data as holotype, 13 ∑∑, leg. H. Zettel (189), 6 ∑∑. B e n g u e t: west of Baguio, at km 7 of Asin Road, 17.II. 1999, leg. S. Schödl (11), 2 ∑∑, leg. H. Zettel (179), 2 ∑∑; Baguio, leg. C.F. Baker, 1 ∑. Description of worker: Measurements: holotype worker: CI 72, HL 2.95, HW 2.12, MdI 58, MdL 1.70, MsL 4.17, PnW 1.25, PtH 1.18, PtL 1.12, PtW 0.49, SI 146, SL 3.08, TL 16.25; paratype worker with smallest HW: CI 72, HL 2.98, HW 2.13, MdI 55, MdL 1.65, MsL 4.00, PnW 1.20, PtH 1.18, PtL 1.18, PtW 0.51, SI 144, SL 3.07, TL 12.75; paratype worker with largest HW: CI 73, HL 3.60, HW 2.62, MdI 59, MdL 2.13, MsL 5.93, PnW 1.52, PtH 1.48, PtL 1.53, PtW 0.56, SI 137, SL 3.58, TL 16.13. Structures: Head striate; striation reaching occipital margin. Pronotum with fine, transverse striation. Mesopleuron with fine transverse striation. Petiole stout, with short spine, bent backwards only very slightly. Pilosity: Pubescence sparse, long. Colour: Head and mesosoma medium brown, petiole and gaster dark brown. Distribution (Fig. 47): Endemic to the Philippines: only in the north of Luzon: Mountain Province, Benguet. Habitats: This species was collected at higher elevations in strongly degraded forests. Notes: Odontomachus schoedli sp.n. is only known from the western mountain ranges of northern Luzon. In Benguet it is sympatric with O. infandus. Main differences between these two species are the striation of the pronotum, which is transverse in O. schoedli sp.n. but more or less longitudinal in O. infandus, and the shape of the petiole, which is remarkably short and with a very straight anterior face of the node (including spine) in O. schoedli sp.n., whereas it has a longer, curved spine in O. infandus (only weakly developed in the smallest specimens). The uniformly light chocolate brown colour of head and mesosoma of O. schoedli sp.n. is striking, but we have seen a few specimens of O. infandus from northern Luzon with the same colouration.Published as part of Sorger, D. M. & Zettel, H., 2011, On the ants (Hymenoptera: Formicidae) of the Philippine Islands: V. The genus Odontomachus Latreille, 1804., pp. 141-163 in Myrmecological News 14 on page 15
Prospecte Der Churfürstl: Saechsl: Residenz Stadt Dresden, Neustadt beÿ Dresden, Friedrichstadt u: Vorstaedte : ingl: derer Maerckte, füernehmsten Plätze und Strassen, Churfl; Herrschafftl: Publiquer auch anderer ansehnl: Gebaeude / Joh: Gotth: Herrmann. excud: Dresdae. Inv: et sculps. D. H. S.
No full textKupfertitel, 75 Ill. (Kupferst.), 1 Kt. (Kupferst.
Transmitância de microondas, como método não-invasivo para a medida da umidade do solo, em rhizobox, utilizando antenas de microfita.
No full textEntrada padronizada: HERRMANN-JUNIOR, P. S. de P
Two-component systems of Corynebacterium glutamicum: Deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response
No full textKocan M, Schaffer S, Ishige T, Sorger-Herrmann U, Wendisch VF, Bott M. Two-component systems of Corynebacterium glutamicum: Deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response. Journal of Bacteriology. 2006;188(2):724-732.Corynebacterium glutamicum contains genes for 13 two-component signal transduction systems. In order to test for their essentiality and involvement in the adaptive response to phosphate (Pi) starvation, a set of 12 deletion mutants was constructed. One of the mutants was specifically impaired in its ability to grow under Pi limitation, and therefore the genes lacking in this strain were named phoS (encoding the sensor kinase) and phoR (encoding the response regulator). DNA microarray analyses with the C glutamicum wild type and the Delta phoRS mutant supported a role for the PhoRS system in the adaptation to P, starvation. In contrast to the wild type, the Delta phoRS mutant did not induce the known Pi starvation-inducible (psi) genes within 1 hour after a shift from P, excess to Pi limitation, except for the pstSCAB operon, which was still partially induced. This indicates an activator function for PhoR and the existence of at least one additional regulator of the pst operon. Primer extension analysis of selected psi genes (pstS, ugpA, phoR, ushA, and nucH) confirmed the microarray data and provided evidence for positive autoregulation of the phoRS genes
Paul Schnabel. Text und Karten des Ptolemäus. (Quellen u. Forschungen zur Geschichte der geographie u. Völkerkunde hgg. von Albert Herrmann, Band 2.)
No full textDept. G.-G. Paul Schnabel. Text und Karten des Ptolemäus. (Quellen u. Forschungen zur Geschichte der geographie u. Völkerkunde hgg. von Albert Herrmann, Band 2.). In: L'antiquité classique, Tome 8, fasc. 1, 1939. p. 300
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