29 research outputs found
The reform of computer professional practice in colleges and universities
Full text linkCombined with the main characteristics and needs of the current development of higher education, this paper analyzes
the importance of college computer professional practice teaching reform, and discusses the existing problems. Comprehensive college
computer professional talent cultivation related content, college computer practical teaching innovation reform to explore, help to improve
the eff ectiveness of computer professional classroom teaching, to achieve computer professional innovation and development
Optimized screening of DNA methylation sites combined with gene expression analysis to identify diagnostic markers of colorectal cancer
No full textAbstract Background The prognosis of patients with colorectal cancer is related to early detection. However, commonly used screening markers lack sensitivity and specificity. In this study, we identified diagnostic methylation sites for colorectal cancer. Methods After screening the colorectal cancer methylation dataset, diagnostic sites were identified via survival analysis, difference analysis, and ridge regression dimensionality reduction. The correlation between the selected methylation sites and the estimation of immune cell infiltration was analyzed. The accuracy of the diagnosis was verified using different datasets and the 10-fold crossover method. Results According to Gene Ontology, the main enrichment pathways of genes with hypermethylation sites are axon development, axonogenesis, and pattern specification processes. However, the Kyoto Encyclopedia of Genes and Genomes (KEGG) suggests the following main enrichment pathways: neuroactive ligand–receptor interaction, calcium signaling, and cAMP signaling. In The Cancer Genome Atlas (TCGA) and GSE131013 datasets, the area under the curve of cg07628404 was > 0.95. For the NaiveBayes machine model of cg02604524, cg07628404, and cg27364741, the accuracies of 10-fold cross-validation in the GSE131013 and TCGA datasets were 95% and 99.4%, respectively. The survival prognosis of the hypomethylated group (cg02604524, cg07628404, and cg27364741) was better than that of the hypermethylated group. The mutation risk did not differ between the hypermethylated and hypomethylated groups. The correlation coefficient between the three loci and CD4 central memory T cells, hematological stem cells, and other immune cells was not high (p < 0.05). Conclusion In cases of colorectal cancer, the main enrichment pathway of genes with hypermethylated sites was axon and nerve development. In the biopsy tissues, the hypermethylation sites were diagnostic for colorectal cancer, and the NaiveBayes machine model of the three loci showed good diagnostic performance. Site (cg02604524, cg07628404, and cg27364741) hypermethylation predicts poor survival for colorectal cancer. Three methylation sites were weakly correlated with individual immune cell infiltration. Hypermethylation sites may be a useful repository for diagnosing colorectal cancer
Atmospheric deposition of antimony in a typical mercury-antimony mining area, Shaanxi Province, Southwest China
No full textMercury-antimony (Hg-Sb) mining activities are important anthropogenic sources of Hg and Sb to the local environment. The Xunyang Hg-Sb mine situated in Shaanxi Province is an active Hg mine in China. To understand the emission, transportation, and deposition of Sb through Hg-Sb mining activities, current study systematically monitored the Sb concentration in precipitation in the Xunyang Hg-Sb mining district. Five groups of experimental pots were carefully designed to further investigate the influence of Hg-Sb mining activities on the Sb contamination in the local surface soil. Based on the overtime increasing of the Sb concentrations in soil from experimental pots, for. the first trial, we estimated the atmospheric deposition flux/mass of Sb in the Xunyang Hg-Sb mining district. Our results showed that the concentrations of Sb in precipitation in the Xunyang Hg-Sb mining district ranged from 0.71 mu g L-1 to 19 mu g L-1 (mean = 4.2 +/- 4.5 mu g L-1), which was orders of magnitude higher than that at the control site. As expected, the concentration of Sb in precipitation was highly elevated near of the Hg-Sb smelter and gradually decreased with distance from the smelter. After 12 months exposure, Sb concentrations in soil of experimental pots were increased by 1.2-8.5 times. The average atmospheric wet and dry deposition flux of Sb in the Xunyang Hg-Sb mining district were 7.2 +/- 6.9 mu g m(-2) day(-1) and 2.1 +/- 4.7 mg m(-2) day(-1), respectively; the annual wet and dry deposition mass of Sb through Hg-Sb mining activities were estimated to be 1.6 t y(-1) and 158 t y(-1), respectively, indicating that dry deposition was the dominant pathway (98 +/- 1.2%) for the removal of Sb from the atmosphere. Our results confirmed that the ongoing Hg-Sb mining activities resulted to serious Sb contamination to terrestrial ecosystems, posing a potential threat to local residents in the Xunyang Hg-Sb mining district. (C) 2018 Elsevier Ltd. All rights reserved
Additional file 11 of Optimized screening of DNA methylation sites combined with gene expression analysis to identify diagnostic markers of colorectal cancer
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Additional file 8 of Optimized screening of DNA methylation sites combined with gene expression analysis to identify diagnostic markers of colorectal cancer
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Additional file 3 of Optimized screening of DNA methylation sites combined with gene expression analysis to identify diagnostic markers of colorectal cancer
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Effects of Microwave-Assisted Annealing on the Structure and Magnetic Properties of (Nd0.75Pr0.25)9Fe72Ti1Zr3Mn x Mo4−x B10.5C0.5 Amorphous Ribbons
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