79 research outputs found
Spray drying technology for the production and processing of microencapsulated omega-3 fish oil with egg powder
No full textProtein with essential amino acids is required for recovering, repairing, and building muscles after intensive exercise. A powder produced with egg white (EW) (high quality protein) and fish oil (menhaden (Brevoortia patronus) (MO) oil or salmon oil (SO)) with high DHA and EPA content should be particularly beneficial for athletes. The objective of this study was to develop microencapsulated omega-3 fatty acids fortified EW powders. Two stable emulsions were prepared with 3.43% (MO) or (SO), 56.21% EW, and 40.36% water (E-MO-EW and E-SO-EW). An EW with water solution (without fish oil) (E-EW) was prepared as a control. Two emulsions (E-MO-EW; E-SO-EW) and E-EW solution were separately spray dried at 130, 140, and 150 oC inlet air temperatures producing three microencapsulated menhaden oil fortified EW powders, three microencapsulated salmon oil fortified EW powders, and three egg white powders (dried E-EW). Physical and chemical properties of E-EW, E-MO-EW and E-SO-EW were determined and the energy used to spray dry them was estimated. The powders were analyzed for color, fatty acids methyl esters (FAME), protein, fat, moisture, ash, amino acid profile, minerals, microstructure and particle size. Microencapsulated efficiency (ME) was estimated only for microencapsulated fish oil fortified EW powders. Triplicate experiments were conducted and data statistically analyzed (á=0.05). The actual production rate of powders ranged from 0.056 to 0.060 (kg dry solids/h). More energy was used to spray dry E-EW, E-MO-EW, and E-SO-EW at 150°C than at 130 and 140oC inlet air temperature. The inlet air temperature did not affect the EPA or DHA content of MO and SO or the microencapsulation efficiency. The protein content of the oil fortified powders was lower than that of the dried E-EW powders. Leucine was the main essential amino acid found in all the powders. Most of the powders’ particles ranged in size from 20 to 30 µm. The study demonstrated that high quality egg white protein with omega-3 can be produced by microencapsulation. Oil fortified egg white powders could provide benefits for athletes who do high intensity exercise. This study also identifies opportunities for development of microencapsulated omega-3 fatty acids fortified egg white powders
Use of an Adsorption Process for Purification of Pollock‐Oil‐Based Biodiesel Comprises Methyl Esters
No full textThe quality of biodiesel from crude pollock oil and the effect of a purification process on the physicochemical properties of pollock oil biodiesel were evaluated. Unpurified pollock oil (PO) was transesterified to biodiesel from pollock oil (BPO) using methyl alcohol (1:6 molar ratio) and NaOH (1 % w/w of the oil weight); and the resulting fatty acids methyl esters (FAME) were purified with 10 % (w/w) activated earth to yield purified biodiesel from pollock oil (PBPO). The samples were evaluated for yield, FAME composition, free fatty acids (FFA), peroxides value (PV), moisture, bulk density, cloud point, flash point, free and total glycerin, color, rheological properties, and minerals. BPO and PBPO were evaluated for the kinetics of lipid oxidation. The transesterification and purification processes had no effect on the FAME composition of PO. The yield of PBPO was significantly (P \u3c 0.05) reduced after the transesterification and purification processes. Moreover, the transesterification process significantly (P \u3c 0.05) reduced the FFA, moisture, bulk density, flash point, total glycerides, redness, viscosity, arsenic and silicon content in PO. Meanwhile, the purification process significantly (P \u3c 0.05) reduced the PV, redness, and sulfur content of BPO. The flash point of BPO was significantly (P \u3c 0.05) increased by the purification process from 79 to 84°C. PO, BPO, and PBPO behaved as non-Newtonian and Newtonian fluids at 0 and 25°C, respectively. BPO showed a lower oxidation rate and activation energy compared to those of PBPO. BPO and PBPO met the ASTM biodiesel standard D6751 for moisture, bulk density, cloud point, free and total glycerin, Na, P, and S. The study demonstrated that high viscosity crude pollock oil could be converted into low viscosity purified pollock oil biodiesel. © 2012 AOCS
Developing Co-current and Counter-current Spray Drying Computational Fluid Dynamics (CFD) Simulation Studies to Predict the Quality of Microencapsulated Fish Oil with Egg White Hydrolysates Powders
No full textEggs are an excellent source of high quality protein containing all essential amino acids (EAA) including leucine. Food-grade protein hydrolysates can enhance the growth of lactic acid bacteria (LAB). Thermal degradation of bioactives normally occurs during microencapsulation by spray drying. Spray drying modeling using computational tools is essential to engineer new food powders and to minimize and thermal degradation of bioactives. The first chapter of this work discusses the optimization of an enzymatic process in terms of reaction temperature, pH, enzyme:substrate ratio, and reaction time using a response surface methodology (RSM) to produce food-grade protein hydrolysates from egg whites (EWH). Resulting EWH produced with proteases from Aspergillus oryzae contained all EAA and showed high antioxidant activity. The EWH were evaluated as a nitrogen source in MRS media for the growth of L. plantarum, L. acidophilus, and L. reuteri. MRS containing EWH had similar performance to that of conventional MRS and produced higher cell yields and better quality of biomass than MRS containing dried egg white proteins. Three-dimensional computational fluid dynamics (CFD) simulations were performed to study the effect of co-current and counter-current spray drying configurations on the quality of microencapsulated fish oil with EWH powders in the last section of this work. Also, the separation of powder particles from drying air at the spray dryer’s cyclone separator was evaluated using CFD. CFD models predicted the drying air flow pattern, particle histories including temperature, residence times (RT), moisture content and particle size of the microencapsulated powders. Predicted moisture content of powders was lower than measured values; however, the predicted mean particle sizes were similar to the measured values. Lower lipid oxidation and microstructure degradation was observed in emulsions dried at 130°C inlet air temperature at feeding rates of 1.0 Kg/h and under counter-current spray drying conditions. The 3D-CFD model predicted lower RT (s) for emulsions dried in counter-current compared to co-current spray drying configurations. The study demonstrated that EWH can be enzymatically produced and can be used as an effective nitrogen source in MRS media. Also, 3D-CFD spray drying modeling can be effectively used to study the moisture evaporation and to predict the final quality of spray dried powders
Recent Advancements in Marine Collagen: Exploring New Sources, Processing Approaches, and Nutritional Applications
No full textCollagen is a structural protein found in the connective tissues of terrestrial and marine animals. Its diverse functional attributes span its applications in several industries, including food, supplements, cosmetics, and pharmaceuticals. Typically derived from mammalian sources, collagen and its derivatives, including gelatin and collagen peptides, are essential for the food and supplement industries. Recently, marine collagen has emerged as a viable mammalian collagen alternative due to its unique functionality and sustainability. Marine vertebrates and invertebrates are reliable sources of marine collagen. Some marine organisms are promising sustainable sources of collagen for nutritional applications. Recent research highlights significant advances in marine collagen extraction, processing, and novel applications. Hence, recent interest has propelled research in identifying novel collagen sources and advancing technologies to produce marine collagen-based products. Considering the recent scientific interest in marine collagen, this review provides an overview of recent progress in marine collagen production, including novel sources, innovative processing technologies, nutritional and functional properties, safety and quality control, current challenges, and future research directions. The review highlights certain challenges, including unpleasant odor, flavor, color, insufficient supply, and inconsistent quality of marine collagen. Future research should focus on increasing the collagen extraction yield, improving the smell and flavor, and developing novel delivery systems to increase bioavailability and functionality
Evaluation of Low-Cost Smartphone-Based Infrared Cameras to Assess the Cooling and Refrigerated Storage Temperatures of Fresh Produce
No full textPopulations of pathogens may increase in fresh produce when subjected to temperature abuse. Smartphone-based infrared (SBIR) cameras are potential alternatives for temperature measurements of fresh produce during postharvest handling and storage. This study compared the performance of SBIR cameras (FLIR and Seek) against conventional temperature acquisition devices for evaluating fresh produce’s simulated hydrocooling and storage conditions. First, thermal images of fresh produce were obtained with SBIR cameras and handheld thermal imagers at ~35 °C, ~20 °C, and ~4 °C to simulate outdoor, packinghouse, and refrigerated environments, respectively. Next, fresh produce was incubated at ~42 °C for 20 h and immersed in chilled water for a hydrocooling simulation. Then, boxes containing cooled fresh produce were stored in a walk-in cooler at different heights for three days. FLIR SBIR cameras were more effective at capturing thermal images of fresh produce than Seek SBIR cameras in all evaluated conditions. More importantly, SBIR cameras accurately acquired temperature profiles of fresh produce during simulated hydrocooling and cold storage. Additionally, the accuracy and quality of thermal images obtained with FLIR cameras were better than those obtained with Seek cameras. The study demonstrated that SBIR cameras are practical, easy-to-use, and cost-effective devices to monitor fresh produce’s temperature during postharvest handling and storage
Ellagitannin content and anti-enterohemorrhagic Escherichia coli activity of aqueous extracts derived from commercial pomegranate products
No full textThis study compared the efficacy of aqueous extracts of commercially available pomegranate peel products and a juice powder in inhibiting the growth of two enterohemorrhagic Escherichia coli strains. Cell suspension of each E. coli strain (5 Log CFU/ml) was added into tryptic soy broth amended with 9 or 23% of each extract prepared with two different methods. After treatment for 5, 10, and 24 h at 25 °C, surviving E. coli cells were enumerated on tryptic soy agar to determine cell population reduction compared to the controls. The concentrations of six different ellagitannins and titratable activity in each treatment system were determined and correlated to E. coli cell population reduction. The extracts from three powdered pomegranate peels caused a significantly greater (p ≤ 0.05) reduction in E. coli population than the extract from the whole peel and juice powder. The higher dose of extracts resulted in a greater cell population reduction than the lower dose. The level of E. coli population reduction correlated positively with the total ellagitannins content (R2 0.67–0.98) and the titratable acidity (R2 0.69–0.98) in the treatment systems. The study suggests that pomegranate peels are promising natural additives or preservatives to control pathogens like EHEC
Inhibition of <i>Salmonella enterica</i> and Enterohemorrhagic <i>Escherichia coli</i> by Ethanolic Extracts of Pomegranate Peels
No full textThis study compared the effect of phenolics extracted from four different types of pomegranate peels for controlling the growth of Salmonella enterica and enterohemorrhagic Escherichia coli. Cells of the two bacterial cultures (5 log CFU/mL) were treated in tryptic soy broth containing 0, 1, or 2% ethanolic extracts of four pomegranate peels for 5, 10, or 24 h at 25 °C. The surviving cells were recovered on a general-purpose agar and a selective medium. The extracts of three products were more effective (p ≤ 0.05) against E. coli than the extract of the remaining product, which had a similar activity to the extracts of two of the products used against S. enterica. Longer treatment time and higher extract concentration resulted in greater pathogen population reductions. The bacterial strains used had varied susceptibility to the extracts. Reductions in cell population positively correlated with the total contents of hydrolyzable tannins in the treatment systems. These findings suggested that ethanolic extracts of evaluated pomegranate peels had inhibitory activities against the two bacterial pathogens. This highlights the potential of pomegranate peels as a promising natural alternative to conventional antimicrobials for controlling foodborne pathogens like S. enterica and E. coli
Chitosan Nanoparticle Penetration into Shrimp Muscle and its Effects on the Microbial Quality
No full textChitosan (CH) and chitosan-sodium tripolyphosphate (CH-TPP) solutions were produced with and without sonication and ultra-shearing. The CH and CH-TPP particles and solutions were evaluated for physicochemical properties, and fluorescently labeled particle penetration into shrimp muscle tissue through vacuum tumbling was observed. Two solutions were prepared: (1) a 0.5 % CH solution in 1 % acetic acid and (2) a CH-TPP solution, prepared by adding 0.167 % sodium tripolyphosphate to the CH solution, instantly forming CH-TPP nanoparticles through ionotropic gelation. Untreated shrimp meat and shrimp meat vacuum tumbled with CH, CH-TPP, acetic acid, sodium tripolyphosphate, and distilled water solutions were analyzed for aerobic plate counts for 24 days of refrigerated storage at 4 °C. Processing with sonication and ultra-shearing reduced the particle sizes of CH and CH-TPP nanoparticles and the molecular weight of CH. It was observed that after processing, fluorescently labeled CH and CH-TPP nanoparticles could penetrate inside of and attach to shrimp muscle tissues through vacuum tumbling. At 24 days of refrigerated storage, shrimp vacuum tumbled with processed CH solution had the lowest aerobic plate counts of all treatments and it was the only treatment to have unchanged microbial quality throughout the entire storage time. Vacuum tumbling with sonicated and ultra-sheared CH solution enhanced particle penetration into shrimp and inhibited microbial growth during refrigerated storage
) Viscera
No full textThe effects of neutralization, transesterification, and adsorption on the physicochemical properties of fatty acid methyl esters (FAME) obtained from crude catfish oil (CO) were investigated. CO was recovered from catfish viscera and subjected to: neutralization (NCO) with 20 (g/100 g) NaOH solution, transesterification (BIO) with methyl alcohol (1:6 molar ratio, oil: alcohol) and 1 (g/100 g) (w/w) NaOH, and purification by adsorption (PBIO) with activated earth (10 g/100 g of BIO). CO, NCO, BIO, and PBIO were evaluated for FAME, free fatty acids (FFA), peroxide value (PV), cloud and flash points, free and total glycerin, rheological properties, and minerals. Catfish viscera had a lipid content of 28.5 g/100 g (wet basis), of which 20.9 g were recovered as CO (73.33% yield). Furthermore, 84.67 g of NCO, 77.53 g of BIO, and/or 60.22 g of PBIO were produced from 100 g of CO processed. NCO had significantly (P \u3c 0.05) lower FFA, density, cloud point, flash point, and diacylglycerols content than CO. Meanwhile, PBIO had significantly (P \u3c 0.05) lower PV and cloud points when compared to BIO. CO, NCO, BIO, and PBIO behaved as Newtonian fluids at 25 °C. BIO and PBIO adhere to the american society for testing and materials (ASTM) biodiesel standards for FAME, moisture, density, cloud point, free and total glycerin, Na, P, and S. This study demonstrated the feasibility of converting high-viscosity CO into low-viscosity PBIO with promising physicochemical attributes. PBIO has the potential to serve as a viable alternative biofuel
Development of cantaloupe (Cucumis melo) juice powders using spray drying technology
No full textCantaloupes are a good source of carotenoids and vitamin C. Cantaloupe fruit juice powder containing vitamin C and β-carotene can be produced by spray drying. The objective of this study was to develop cantaloupe fruit juice (CJ) powder and to evaluate its nutritional and physical properties. Fresh cantaloupe fruits purchased from a local store were diced and juiced. CJ with 10% maltodextrin (MD) added was spray dried at inlet temperatures of 170, 180 and. /or 190 °C. Three spray dried cantaloupe powders, including CJ dried with MD at 170 °C (CJP1), CJ dried with MD at 180 °C (CJP2), CJ dried with MD at 190 °C (CJP3) were analyzed for moisture, water activity, vitamin C, β-carotene, dissolution, and microstructure. The actual production rate of the cantaloupe juice powders was lower than the estimated production rate. CJP1 had (p \u3c 0.05) higher moisture content and water activity than CJP2, and CJP3. Vitamin C content (mg/100 g, dry solids) was significantly (p \u3c 0.05) higher in CJP1 (136.36 ± 5.84) than CJP2 (91.85 ± 5.23) and CJP3 (78.30 ± 1.96). The powder produced at inlet temperatures 170 °C had higher β-carotene content (μg/g) than that produced at 180 and 190 °C. © 2011 Elsevier Ltd
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