1,721,183 research outputs found
Role of ethylene in Axillary Shoot Proliferation of Lavandin-Intercation with Benzyladenine and Polyamines
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In vitro propagation and shoot encapsulation as tools for ex situ conservation of the aquatic plant Ludwigia palustris (L.) Ell.
Full text linkEndogenus ethylene requirement for adventitious root induction and growth in tomato cotyledons and lavandin microcuttings in vitro
No full textLeaf ultrastructure, photosynthetic rate and growth of myrtle plantlets under different in vitro culture conditions
No full textDevelopment of autotrophy and tolerance to acclimatization of myrtus communis transplants cultured in vitro under different aeration
No full textThe behaviour of myrtle (Myrtus communis L.) plantlets during the last phase of in vitro culture before transplanting was studied. Myrtle plants were sampled from Mediterranean shrubland vegetation. In vitro growth of myrtle microcuttings was evaluated during the rooting phase using 500 cm3 containers fitted with two different types of closures. The number of gas exchanges and time in which aerated and closed vessels lose half of their gas content were calculated. Both types of vessel closure allowed photosynthetic activity in myrtle cultures even though the higher aeration rate induced higher net photosynthetic rate (PN) during all the culture. In vitro morphogenetic ability and rooting of myrtle microcuttings were affected by the different environment conditions inside the culture vessels: plantlet growth and root formation of myrtle explants increased in aerated vessels in comparison with closed ones. The well developed root system, the higher PN and dry mass accumulation during the pre-acclimatization phase in aerated vessels induced a better ability to face the transplant stress
Establishment of in vitro tissue cultures from Echinacea angustifolia D.C. adult plants for the production of phytochemical compounds
No full textThe establishment of in vitro cultures of Echinacea angustifolia D.C. was obtained directly from sections of flower stalks of adult plants. The shoot formation was obtained from this plant material placed on a modified MS basal medium named CH supplemented with 0.5 mg L1 6-benzylaminopurine (BA). The in vitro propagation procedure of E. angustifolia consisted of three distinct phases: an initial regeneration phase from stalk sections (IP shoots on basal medium with 0.25 mg L1 BA), an elongation phase on active charcoal and an axillary proliferation of the shoots (AP shoots on basal medium with 0.5 mg L1 BA).
Regenerating calli were established from leaves of in vitro shoots cultured on CH medium supplemented with 3 mg L1 BA and 0.5 mg L1 indole-3-butyric acid (IBA). Developed shoots from the callus cultures were subcultured on the CH medium with 0.5 mg L1 BA (leaf regenerated shoots: LR shoots). The secondary metabolite content of the in vitro plant material was compared with that of the greenhouse growing plants. The quali-quantitative LC-DAD-ESI-MS analysis on the extracts from axillary proliferation shoots (AP shoots) showed significant production of caffeic acid derivatives while leaf callus and LR shoots, accumulated mainly alkamides. These results showed that the proper choice of the procedures for in vitro multiplication allowed us to obtain plant biomass able to produce the active compounds typical of E. angustifolia plants
Variazione del contenuto dei principali flavonoidi in foglie del mutante di pomodoro Never Ripe (NR) insensibile all’etilene in risposta a stress da ozono
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