6,925 research outputs found
Nextgrid architectural concepts
No full textThis paper outlines the conceptual model of the NextGRID architecture. This conceptual model consists of a set of architectural principles and a simple decomposition of the architecture in order to facilitate common understanding of the architecture and its development
Landsat MSS classification of fire fuel types in Wood Buffalo National Park, northern Canada
No full textJ1: Global Ecology & Biogeography Letters; M3: Article; Milne, David Franklin, Steven E. Wilson, Bradley A. Ghitter, Geoff Heathcott, Mark McCaffrey, Thomas M. Ow, Charlotte F. Y.; Source Information: Mar1994, Vol. 4 Issue 2, p33; Subject Term: FOREST fires; Author-Supplied Keyword: Canada (Wood Buffalo National Park); Author-Supplied Keyword: Forest fire; Author-Supplied Keyword: Fuel type classification; Author-Supplied Keyword: Landsat data; Number of Pages: 0p; Document Type: Articl
Dynamics and folding of single two-stranded coiled-coil peptides studied by fluorescent energy transfer confocal microscopy
No full textWe report single-molecule measurements on the folding and unfolding conformational equilibrium distributions and dynamics of a disulfide crosslinked version of the two-stranded coiled coil from GCN4. The peptide has a fluorescent donor and acceptor at the N termini of its two chains and a Cys disulfide near its C terminus. Thus, folding brings the two N termini of the two chains close together, resulting in an enhancement of fluorescent resonant energy transfer. End-to-end distance distributions have thus been characterized under conditions where the peptide is nearly fully folded (0 M urea), unfolded (7.4 M urea), and in dynamic exchange between folded and unfolded states (3.0 M urea). The distributions have been compared for the peptide freely diffusing in solution and deposited onto aminopropyl silanized glass. As the urea concentration is increased, the mean end-to-end distance shifts to longer distances both in free solution and on the modified surface. The widths of these distributions indicate that the molecules are undergoing millisecond conformational fluctuations. Under all three conditions, these fluctuations gave nonexponential correlations on 1- to 100-ms time scale. A component of the correlation decay that was sensitive to the concentration of urea corresponded to that measured by bulk relaxation kinetics. Thetrajectories provided effective intramolecular diffusion coefficients as a function of the end-to-end distances for the folded and unfolded states. Single-molecule folding studies provide information concerning the distributions of conformational states in the folded, unfolded, and dynamically interconverting states.Author manuscript. Published in final edited form as: Proc Natl Acad Sci U S A. 2000 November 21; 97(24): 13021-13026.The final published version of this article is located at: http://www.pnas.org/cgi/reprint/97/24/13021NIH GM54616; to William F. DeGradoNIH GM12592; to Robin M. HochstrasserNIH GM48130; to William F. Degrado and Robin M. HochstrasserThis work was supported by GM54616 (to W.F.D.), GM12592 (to R.M.H.) and GM48130 (to W.F.D. and R.M.H.) with instrumentation developed under RR01348. D.S.T. was supported by National Institutes of Health Grant NRSA F32-GM18589.Also available in PubMed Central. PMCID:PMC2717
Collagen fibrils in human tendons, is everything already said? A qualitative ultrastructural assessment
No full textImproving structural knowledge of human tendons
is fundamental for a better understanding of pathological
and regenerative processes occurring in these key
structures. Recent advances in tendon biology indicate
cellular heterogeneity between tendons of different muscles.
In contrast, very little is known about potential differences
in extracellular matrix in tendons across anatomy.
In the present work, we aimed to assess collagen
fibril organisation ultrastructurally in human supraspinatus,
semitendinosus, and quadriceps tendons, as well
as in the anterior cruciate and patellar ligaments. Samples
were obtained with appropriate permissions from
seven different donors belonging to the Anatomy Gift
Programme of the Royal College of Surgeons in Ireland
in Dublin, that had been previously embalmed for routine
anatomical examination.
Samples from the tendon mid-body were harvested
and processed for Transmission Electron Microscopy
(TEM).
We examined a total of 34 tendons (n=2 samples of
each). Collagen fibrils were well recognisable in 32 tendons.
O
verall, marked heterogeneity between samples
from the same tendon type of different donors, or
between the various tendons of the same donor, was
observed in terms of collagen fibril shape (rounded
vs more irregular-polygonal), size distribution, and
width. The greatest similarity in fibril organisation was
observed between patellar ligament and quadriceps tendon.
The anterior cruciate ligament showed smaller,
less rounded fibrils, which were more consistent in size.
Supraspinatus also showed bundles perpendicular to the
longitudinal tendon axis. Consistency in fibril organisation
between both samples of the same individual tendon
was observed in 26 cases.
This study demonstrates that ultrastructural analysis
of collagen fibril is feasible in tissue from anatomical
donors despite the embalming process not being
a standard procedure of fixation for TEM examination.
This first description also offers a basis for a more
detailed and quantitative assessment of the collagen
fibrils in human tendons.
References
1) Franchi, Marco, Trirè, Alessandra, Quaranta, Marilisa,
Orsini, Ester, Ottani, Victoria. Collagen Structure
of Tendon Relates to Function. The Scientific World
Journal, 2007, 7, 132725.
2) Christopher K. Revell, Oliver E. Jensen, Tom Shearer,
Yinhui Lu, David F. Holmes, Karl E. Kadler. Collagen
fibril assembly: New approaches to unanswered questions.
Matrix Biology Plus, 2021, 12, 100079.
3) Baldwin M, Buckley CD, Guilak F, Hulley P, Cribbs
AP, Snelling S. A roadmap for delivering a human
musculoskeletal cell atlas. Nat Rev Rheumatol. 2023
19, 738-752
Fort Snelling, Minnesota, USA, monthly climate normals comparisons.
No full text<p>Shown are the differences between the AD 1895–1924 temperature means minus the AD 1840–1869 temperature means, and the AD 1961–1990 temperature means minus the AD 1840–1869 temperature means for each month and annually. Temperatures in °C; two-sided <i>t</i>-tests assuming equal variances, with 58 degrees of freedom; bold denotes significance at the 0.05 level.</p><p>Fort Snelling, Minnesota, USA, monthly climate normals comparisons.</p
Fly about round me coursing, swallow sweet birds come near [first line]
No full textstrophicpiano and voiceCover is duplicated in 125.115b.Johns Hopkins University, Levy Sheet Music Collection, Box
125, Item 115aTranslated From the French of Volney L'Hotelier by Samuel J. Gardner, Esq. The Music by Felicien David (Author of "Le Desert").E.G. Warren, Engr
Fly about round me coursing, swallow sweet birds come near [first line]
No full textstrophicpiano and voiceCover is duplicated in 125.115b.Johns Hopkins University, Levy Sheet Music Collection, Box
125, Item 115aTranslated From the French of Volney L'Hotelier by Samuel J. Gardner, Esq. The Music by Felicien David (Author of "Le Desert").E.G. Warren, Engr
Cult: A Composite Novel
No full textCult (redacted)
The first component of the thesis is a composite novel called Cult which falls into two parts with seven narratives in each. Part 1 tracks the protagonist, Ellen, from her first involvement with the cult through to her eventually leaving it. Although fiction, the first half of the book answers the kinds of questions the author is asked when people discover that she was once a sannyasin (a follower of the guru Bhagwan Shree Rajneesh). While the experiences of meditation, group therapy and communal living are all faithfully rendered within the stories, the need for strong characters, narrative drive and a lightness of touch takes precedence.
Part 2 picks up Ellen’s story some twenty or so years later and explores what becomes of her in middle age. It also looks at other groups in society, such as academia, the law and the internet dating community which each have their own jargon, hierarchies, rituals and rules but are not considered to be cults.
The book examines the question raised in the Epigraph, ‘how do we be together when we feel so alone’ with a focus on relationships other than the familial and the romantic.
Collisions, Chasms and Connections: a Performative Exploration of the Composite Novel Form
The second part of the thesis is both a critical and creative response to three contemporary American books: Olive Kitteridge by Elizabeth Strout; A Visit from the Goon Squad by Jennifer Egan; and Legend of a Suicide by David Vann. The critical element comprises a close reading of the three books; a chronological reconstruction of their overarching storylines; and a consideration of what their authors have said about writing the books. It concludes that, in the composite novel, the simultaneous presentation of multiple views and storylines operate much like a 3D image to give the impression of depth to the characters and situations rendered. The creative element of the essay is a playful and personal response to the texts
Towards a lightweight generic computational grid framework for biological research
No full textBackground: An increasing number of scientific research projects require access to large-scale computational resources. This is particularly true in the biological field, whether to facilitate the analysis of large high-throughput data sets, or to perform large numbers of complex simulations – a characteristic of the emerging field of systems biology. Results: In this paper we present a lightweight generic framework for combining disparate computational resources at multiple sites (ranging from local computers and clusters to established national Grid services). A detailed guide describing how to set up the framework is available from the following URL: http://igrid-ext.cryst.bbk.ac.uk/portal_guide/. Conclusion: This approach is particularly (but not exclusively) appropriate for large-scale biology projects with multiple collaborators working at different national or international sites. The framework is relatively easy to set up, hides the complexity of Grid middleware from the user, and provides access to resources through a single, uniform interface. It has been developed as part of the European ImmunoGrid project
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