87,374 research outputs found

    Slik activates Moesin at the luminal membrane in terminal branches.

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    <p>(A–A″) Region of a terminal branch from a third intar larva showing Slik (A) and activated pMoesin (A′) staining. Slik colocalizes with pMoesin at the luminal membrane (A″). The terminal cell is labeled with cytoplasmic GFP, pseudo-colored in blue (A″). (B–C″) pMoesin staining in control (B′) and Slik-depleted (C′) terminal cells. No pMoesin is seen at the luminal membrane in Slik-depleted cells (C′). (D–E″) Higher magnification of a control cell (E) and an example of a <i>slik</i>-RNAi expressing terminal cell (D) with residual Slik staining but no pMoesin. (F–G″) In <i>slik</i><sup>1</sup> homozygous mutant embryos pMoesin staining is present in some branches (F′, green arrowhead) and reduced or absent (F′, white arrowhead) in others. The tracheal tubes are labeled with CBD-Alexa 633 (F′, F″, G and G″, pseudo-colored, in blue). The larval terminal cell is labeled with cytoplasmic GFP, pseudo-colored in blue (D″ and E″). A–C″ and F–G″ are projections of confocal image stacks. D–E″ are single focal planes from image stacks. Scale bars: (A–A″ and D–E″) 5 µm, (B–C″) 20 µm, (F–G″) 20 µm.</p

    Overactivation of FGF signaling overrides requirement for Slik in terminal cell branching.

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    <p>(A–D) Moesin (A, B) and Slik (C–D) staining in larvae expressing constitutively active FGFR alone (λ<i>btl</i>, A′) or in combination with <i>slik</i>-RNAi (B′). λ<i>btl</i>, induces excessive branching; pMoesin and Slik staining are detectable in the λ<i>btl</i> expressing cells, (A′, C′) but absent if <i>slik</i>-RNAi is co-expressed(B′, D′). Depletion of Slik in λ<i>btl</i> expressed terminal cells does not affect expression or membrane localization of total Moesin (E and F′). A–E″ are projections of confocal image stacks. F–F″ are single focal planes from a image stack. Scale bars: (A–E″) 30 µm, (F–F″) 5 µm.</p

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    [Newspaper Clipping: Author Claims Evidence of Second JFK Assassin #1]

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    Newspaper article titled "Author Claims Evidence of Second JFK Assassin." The article states that author Richard J. Whalen concluded "that there is circumstantial evidence to support the theory of a second assassin in the shooting of President John F. Kennedy.

    Slik vil kalven ha det Del 2

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    Erfaringar viser at det er alt for vanleg å sjå bort frå kalven ved planlegging av mjølkeproduksjonsfjøs. Denne artikkelen bygger vidare på artikkelen i Buskap nummer 8 i 2008 "Slik vil kalven ha det". Den artikkelen hadde fokus på kva som er naturleg åtferd hos kalvar, og korleis dei minsta kalvane som går med kua vil ha det. Denne artikkelen tar for seg kalvane etter at dei er skilt frå kua

    Also By The Same Author: AKTiveAuthor, a Citation Graph Approach to Name Disambiguation

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    The desire for definitive data and the semantic web drive for inference over heterogeneous data sources requires co-reference resolution to be performed on those data. In particular, name disambiguation is required to allow accurate publication lists, citation counts and impact measures to be determined. This paper describes a graph-based approach to author disambiguation on large-scale citation networks. Using self-citation, co-authorship and document source analyses, AKTiveAuthor clusters papers, achieving precision of 0.997 and recall of 0.818 over a test group of eight surname clusters

    John F. Kennedy telegram to Roosevelt

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    Jersey Homesteads (later the Borough of Roosevelt) was established in the 1930s as an agro-industrial cooperative community. It was established specifically for urban Jewish garment workers, many of whom had emigrated from Europe. President John F. Kennedy sent a telegram to the citizens of Roosevelt, New Jersey, apologizing for not being able to attend the memorial dedication in honor of former President Franklin Delano Roosevelt. (Jersey Homesteads became Roosevelt in 1945 in honor of the president.) President Kennedy expressed his gratitude to the people of Roosevelt for constructing the memorial, and commented that it will serve as a constant reminder of Roosevelt's good works

    Nonautonomous Stimulation of Cell Proliferation by Slik-Expressing Cells

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    (A, D, G) ptcGAL4 UAS-GFP wing discs. (B, E, H) ptcGAL4 UAS-slik UAS-GFP UAS-p35 wing discs. (C, F, I) ptcGAL4 UAS-slikkd UAS-GFP UAS-p35 wing discs. (A–C, G–I) BrdU incorporation (red). (A–C) Projections of several optical sections. (G–I) Sections of the overlying peripodial layer. (D–F) Peripodial cell nuclei visualized by DAPI. Arrows show high nuclear density above the ptcGAL4 UAS-GFP stripe in the columnar epithelium in (E) and (F). Asterisks indicate the peripodial extension of the ptcGAL4 stripe. (H and I) Cells in this region have incorporated more BrdU than control disc in (G).</p

    Logarithmic variance profiles and the corresponding f-1 spectra of temperature fluctuations in turbulent Rayleigh-Bénard convection

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    We report experimental results for the temperature variance 2(z) and the corresponding frequency spectra P(f) in turbulent Rayleigh-Bénard convection (RBC) in a cylindrical sample of aspect ratioT= D/L = 1:00 (D = 1:12 m is the diameter and L = 1:12 m the height). The measurements were conducted in the Rayleigh-number range 1011 < Ra < 1:35 1014 and Pr ' 0:8. For Ra = 1:35x1014, 2(z) could be described well by a logarithmic dependence on the vertical position z in a range of z 1 < z < z 2 with z 1 ' 70 and z 2 = 0:1L. Here L=(2Nu) is the thickness of a thin thermal sublayer adjacent to the horizontal plate where the heat flux (denoted by the Nusselt number Nu) is carried mostly by thermal diffusion. In the log layer, we found that the temperature spectra had a significant frequency range over which P(f) f with close to 1. As Ra decreased, increased so that the log layer became thinner. At Ra = 2:05 1011, z 2 < z 1 and therefore there was no range for a log layer. Correspondingly, the temperature spectrum near the horizontal plate did not have the f1 scaling form either

    Maine author Franklin F. Gould recalls his first glimpse of the outside world

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    Maine author Franklin F. Gould recalls his first glimpse of the outside world as he relates how, as a young farm boy in the late 1800\u27s, he drove his father\u27s horses on an errand to an icebound river
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