122,586 research outputs found
SiSSA - An Infrastructure for NLP Application Development
No full textRecently there has been a growing interest in infrastructures for sharing NLP tools and resources. This paper presents SiSSA, a project that aims at developing an infrastructure for prototyping, editing and validation of NLP application architectures. The system will provide the user with a graphical environment for (1) selecting the NLP activities relevant for the particular NLP task and the associated linguistic processors that execute them; (2) connecting new linguistic processors to SiSSA; (3) checking that the chosen architectural hypothesis corresponds to the functional specifications of the given application
Predicting structural determinants and Ligand poses in proteins involved in neurological diseases: bioinformatics and molecular simulation studies
No full textPart I presents the computational tools used in this work: the comparative modeling and molecular
docking approaches along with molecular dynamics.
Part II presents structural predictions of Ca2+-binding domains in Ca2+-gated channels. A detailed
description of the structure and function of these proteins can be found in the following Chapters.
Chapter 4 focuses on human large conductance Ca2+- and voltage-gated potassium channel (hBKCa).
Bioinformatics approaches and MD simulations were used to construct models of two domains important
for Ca2+ binding and channel gating, namely the Regulator of Conductance for K+ (RCK1)
and the so called calcium bowl. The relevance of these models for interpreting the available molecular
biology data is then discussed.
Chapter 5 deals with bestrophins, a recently discovered family of Cl− channels. Bestrophins
feature a well conserved Asp-rich tract in their C-terminal part, which is homologous to Ca2+-binding
motifs in calcium bowl of hBKCa. Based on these considerations, we constructed homology models
of human bestrophin-1 Asp-rich domain. MD simulations and free energy calculations were used
to identify Asp and Glu residues binding Ca2+ and to predict eects of their mutations to Ala.
My work, performed in collaboration with C. Anselmi (SISSA/ISAS), was complemented by free
energy calculations carried out by F. Pietrucci (SISSA/ISAS). Selected mutations were investigated
by electrophysiological experiments performed by Prof. A. Menini, J. Rievaj, F. W. Grillo, and A.
Boccaccio (SISSA/ISAS). The model of Asp-rich domain was then validated against experimental
results.
Part III is devoted to the prion protein. In this Part, Chapter 6 presents in vitro studies of D18scFv
anti-prion effects performed by groups of Prof. C. Zurzolo (Institut Pasteur, Paris, France), Prof.
G. Legname (SISSA/ISAS), L. Zentilin and M. Giacca (ICGEB, Trieste, Italy) and by Prof. S. B.
Prusiner (Institute for Neurodegenerative Diseases, University of California San Francisco, U.S.A.) and structural prediction of a complex between the small antibody fragment (D18scFv) and PrPC.
The complex was modeled using bioinformatics approaches. Initially, the D18scFv fragment alone was
modeled based on a similar antibody-fragment template and then docked with prion protein. Based
on this, interactions relevant for the recognition between the two proteins and for the mechanism of
action of D18scFv are discussed.
Chapter 7 describes a computational protocol for the design of ligands targeting cavity-less proteins,
like most proteins involved in neurodegenerative diseases. Molecular docking methods are combined
with MD simulations and free energy calculations using the metadynamics method [33, 34] to gain
insights in ligand binding to such proteins, in our case to prion protein. We focused on a compound
showing antiprion activity in vitro. Ligand-target interactions and ligand binding affinity as emerged
by using our approach are compared with the available NMR data [35] and experimental constant of
dissociation [35]. In this work, also other two students and one postdoc were involved beside myself,
namely S. Bongarzone, G. Rossetti and X. Biarnes (SISSA/ISAS).
Finally, the conclusions are drawn in the last Chapter. The thesis closes with the List of publications
and with the Acknowledgments
SiSSA: An Infrastructure for Developing NLP Applications
No full textIn recent years there has been a growing interest in the commercial deployment of NLP technologies. This paper presents SiSSA, a project whose main aim is that of developing an infrastructure for prototyping, editing and validation of NLP application architectures. The system will provide the user with a graphical environment for (1) selecting the NLP activities relevant for the particular NLP task and the associated linguistic processors that execute them; (2) connecting new linguistic processors to SiSSA; (3) checking that the chosen architectural hypothesis corresponds to the functional specifications of the given application. The proposed infrastructure makes crucial use of state-of-the-art software technologies (CORBA, XML, RDF) to integrate different linguistic processors in an effective way. In the paper the definition of a metaformalism for the unification of different formalisms for grammar description is also briefly presented
SiSSA: A Software Infrastructure for Developing Distributed NLP Applications
No full textIn recent years there has been a growing interest in the commercial deployment of NLP technologies. This paper presents SiSSA, a project that aims at developing a system for prototyping, editing and validation of NLP application architectures. The system will provide the user with a graphical environment for (1) selecting the NLP activities relevant for the particular NLP task and the associated linguistic processors that execute them; (2) connecting new linguistic processors to SiSSA; (3) checking that the chosen architectural hypothesis corresponds to the functional specifications of the given application
Green ICT: trends and challenges
No full textThe papers included in this issue's monograph aim to show how the ICT sector can improve its own energy use and management or help, by means of applications and services, to efficiently manage resources in other high energy consuming sectors. Achieving a sustainable economy has become an increasingly important issue of concern in the developed world. The ICT sector can play a significant supportive role for future economic growth, by contributing innovative solutions for achieving a productive model based on sustainable developmen
Green ICT : trends and challenges
No full textThe papers included in this issue's monograph aim to show how the ICT sector can improve its own energy use and management or help, by means of applications and services, to efficiently manage resources in other high energy consuming sectors. Achieving a sustainable economy has become an increasingly important issue of concern in the developed world. The ICT sector can play a significant supportive role for future economic growth, by contributing innovative solutions for achieving a productive model based on sustainable developmen
FCNC transition of Sigma(Q) to nucleon
No full textAzizi, Kazem (Dogus Author) -- Conference full title: 35th International Conference of High Energy Physics, (ICHEP) 2010; Paris; France; SISSA; 22 July 2010 throught 28 July 2010.The loop level flavor changing neutral current transitions of the Sigma(b) -> nl(+)l(-) and Sigma(c) -> p l(+)l(-) are investigated in full QCD and heavy quark effective theory in the light cone QCD sum rules approach. Using the most general form of the interpolating current for E-Q, Q = b or c, the transition form factors are calculated using two sets of input parameters entering the nucleon distribution amplitudes. The obtained results are used to estimate the decay rates of the corresponding transitions
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