1,178 research outputs found
Springfield College Gymnastic Legacy chart, by A.B. Frederick (Sept. 20, 1991)
No full textThe Springfield's Gymnastic Legacy chart prepared by A. B. Frederick, curator of the International Gymnastics Hall of Fame. The Chart is 77" x 8 1/2". It is printed on folded printer paper. It is signed by the author A.B. Frederick and signed. The chart uses information & from A.B. Frederick's book "Roots of American Gymnastics." This is not a direct representation of the charts in the various additions of the book "Roots of American Gymnastics" - a version of the list, with corrections and additions, can be seen on page 20 and 21 of the 1996 publication. The chart is a genealogical representation of coaches and outstanding gymnasts who have been associated with Springfield College and their history or contributions to the gymnastics, as well as those they have influenced. Honorees of the American National Gymnastics Hall of Fame are found on the charts along with the primary reasons for their election. At the top of the chart is Leslie Judd, the director and founder of the exhibition team at Springfield College. Judd was appointed as the first gymnastic coach and director at Springfield College in 1921. To the left of Judd are the people were were associated with early gymnastics programs at Springfield College before Judd was appointed as coach and director. These people include Henry Kallenberg, Amos Alonzo Stagg, James Naismith, Luther Gulick, and Louis C. Schroeder. Under Judd, stems all the major influencers of gymnastics that he trained. These people include Rene Kern, Charles Graves, Fred Zitta, Ted Steeves, Shotzbarger, Lyle Welser, Wilber West, Ralph Piper, Ray Heidloff, Hartly D'Oyley Price, Tom Dunkley, Walter Ersing, Erik Kjeldsen, Richard Aronson, Tom DeCarlo, and Frank Wolcott. The far right has the most recent history at the time that it was created, including some of the Women's history, including Diane Potter, Mimi Murrary, and Olympian Kathy Corrigan. Not represented is 35 year Women's Head Coach, Cheryl Raymond.Note: the image has been created using multiple photographs that have been pieced together and doctored using Photoshop and Adobe Lightroom; This is not a direct representation of the charts in the book "Roots of American Gymnastics"
Regional economic inequalities; migration and community response, with special reference to Yugoslavia
No full textYesAfter a general introduction to the problems of regional imbalance, this paper proceeds to an analysis of the background and causes of regional economic inequalities in Yugoslavia. Demographic factors are outlined with reference to Yugoslav statistical sources, and the policies being adopted for those areas defined as being in need of special assistance are examined. The author concludes by indicating some lessons to be drawn from Yugoslavia's experience of migration and especially of its workers abroad
Sequence conservation in the RNA polymerase gene of infectious bursal disease viruses
No full textEleven of thirteen infectious bursal disease viruses (IBDV) were shown to be similar following polymerase chain reaction (PCR) amplification of two regions (293 bp and 651 bp cDNA fragments) in genomic segment B and restriction enzyme analysis of the PCR products. Two IBDV strains differed from the eleven viruses when examined by BstEII digestion of the 293 bp PCR product, but all thirteen viruses were similar using the 651 bp cDNA fragment and digestion with AvaI. Because segment B encodes replicating enzyme(s), the results suggest that its sequences may be highly conserved among IBDV isolates.LR: 20061115; PUBM: Print; GENBANK/L19502; JID: 7506870; 0 (DNA Primers); 0 (RNA, Double-Stranded); EC 2.7.7.6 (DNA-Directed RNA Polymerases); EC 3.1.21.- (endodeoxyribonuclease EcaI); EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific); ppublishSource type: Electronic(1
Determination of the 5' and 3' terminal noncoding sequences of the bi-segmented genome of the avibirnavirus infectious bursal disease virus
No full textTerminal sequences of the bi-segmented dsRNA genome of 3 different strains of infectious bursal disease virus (IBDV) were analyzed by the rapid amplification of cDNA 5' ends (5'RACE) procedure. Both segments are 85% homologous in a 32-nucleotide sequence comprising the 5' end, whereas the 3' end has a conserved pentamer. Comparison to published terminal sequences of other IBDV strains revealed high conservation between the two segments but more serotype-specific nucleotide changes (5 on segment A and 3 on segment B) in the 5' noncoding region compared to the 3' noncoding region (none on segment A and 1 on segment B).LR: 20061115; PUBM: Print; GENBANK/U30818; GENBANK/U30819; JID: 7506870; 0 (DNA, Viral); 0 (RNA, Viral); 0 (Viral Proteins); ppublishSource type: Electronic(1
Evidence that virion-associated VP1 of avibirnaviruses contains viral RNA sequences
No full textThe VP1 encoded by genomic segment B of birnaviruses is generally known to exist as a genome-linked protein (VPg) and as a 'free' polypeptide of 90 kDa in virus particles. The guanylylation activity associated with infectious bursal disease virus (IBDV) was demonstrated by incubating purified virus in presence of [alpha 32P] GTP; optimum activity in the 90 kDa form of VP1 was seen in low salt concentration in the presence of 4 mM magnesium ions over a wide range of incubation temperatures. The IBDV VP1 was shown to lack guanyl transferase activity. Northwestern (RNA-protein) blot analysis of purified virus using a radiolabelled cDNA probe consisting of 3' and 5' ends of genomic segment B indicated that both forms of virion-associated VP1 contained viral RNA sequences of which those linked to VPg corresponded to the two genome segments and those linked to the 90 kDa VP1 were probably a short oligonucleotide of the terminal viral RNA sequences.LR: 20061115; PUBM: Print; JID: 7506870; 0 (Capsid Proteins); 0 (RNA, Viral); 0 (Viral Core Proteins); 86-01-1 (Guanosine Triphosphate); EC 2.7.7.- (Nucleotidyltransferases); EC 2.7.7.- (guanylyltransferase); ppublishSource type: Electronic(1
The 5'-terminal 32 basepairs conserved between genome segments A and B contain a major promoter element of infectious bursal disease virus
No full textThe regions of the infectious bursal disease virus (IBDV) genome with regulatory function are not known. In the present study, progressively deleted lengths of the 5' noncoding region of segment A were constructed in pGL3 vectors having SV40 enhancer or promoter, and a luciferase (LUC) reporter gene. Transient transfections of the constructs made in a promoter-less pGL3-Enhancer vector when transfected in Vero cells and the lysates assayed for LUC expression, allowed the localization of maximal activity to the 32-nucleotide stretch (precursor polyprotein ORF positions -131 to -100), which is highly conserved at the 5' end of both genome segments. This fragment, when evaluated in parallel in an enhancer-less pGL3-Promoter vector demonstrated no activity. To determine if this region is recognized by IBDV replicative proteins, we engineered modifications in an enhancer-less pGL3-Promoter vector where the terminal 32-bp fragment, the full-length noncoding region, or the noncoding region with the 32-bp fragment deleted was positioned in either the plus-sense or the minus-sense orientation immediately downstream of the SV40 promoter and upstream of the LUC gene. Transfections of these constructs in IBDV-infected and uninfected Vero cells resulted in the endogenous generation of recombinant viral-LUC RNAs containing the 5' terminal viral RNA sequences in either the plus-sense or the minus-sense orientation. LUC assays of the infected cell lysates showed up-regulated expression of LUC only with constructs containing the 32-bp fragment in the minus-sense orientation. Deletion of this 32-bp fragment abolished such LUC expression. We therefore conclude that the 5'-terminal 32 base pairs of genomic segment A contain a major promoter element in IBDV. In addition, our results show that IBDV replicative proteins recognize and transcribe single-stranded RNA in vivo.LR: 20061115; PUBM: Print; JID: 7506870; ppublishSource type: Electronic(1
Hijacking Frederick Douglass: How Conservatives Exploit His Legacy for Political and Ideological Gain
No full textConservatives distort Frederick Douglass’s legacy to advance political and ideological narratives that contradict his advocacy for racial justice and civil rights. Among them, Black conservatives invoke Douglass to justify their opposition to diversity, equity, and inclusion efforts while appealing to reactionary audiences. By stripping his writings of historical context, they reshape his message in ways that obscure his calls for systemic change. Some exploit his name and image for financial gain. As Douglass’s great-great-great-grandson, the author directly confronts these distortions with historical evidence and personal insight. This work calls for an honest engagement with Douglass’s full legacy and challenges those who manipulate his words for political convenience
Historic buildings of America
No full textxiv, 341 pages, 48 unnumbered leaves of plates : illustrations. Curated title for Fleet Library Special Collections book cover exhibition Bound to Please, fall 2022. Forty seven buildings are described, largely in Massachusetts, with some more in other New England and Mid-Atlantic States, one in California, two in South Carolina, and several in both Canada and Mexico. CONTENTS: The Capitol, Washington / Joseph B. Varnum -- Within the Capitol / Charles Dickens -- Arlington, Virginia / Iza Duffus Hardy -- Carpenter\u27s Hall / Benson J. Lossing -- The Cradock House, Medford / Samuel Adams Drake -- Fraunces Tavern / William J. Davis -- William and Mary College / John Fiske -- The Mission Dolores, San Francisco / Lady Hardy -- King\u27s Chapel, Boston / F. W. P. Greenwood -- Some buildings in Havana -- Richard Davey -- St. Michael\u27s Church, Charleston / William Gilmore Simms -- The Carlyle House, Alexandria -- Independence Hall, Philadelphia / D. W. Belisle -- The Castle of Chapultepec / Thomas Unett Brocklehurst -- Parliament Buildings, Ottawa / Lady Hardy -- Mount Vernon / Arthur Shadwell Martin -- The Old Manse, Concord / Nathaniel Hawthorne -- The Jamestown Tower / Charles Frederick Stansbury -- Nassau Hall, Princeton -- Castle Garden, New York / Esther Singleton -- Monticello / Edward C. Mead -- The William Penn House, Philadelphia / John F. Watson -- The Cathedral, Mexico / Thomas Unett Brocklehurst -- The Whipple House, Ipswich / W. H. Downes -- Fort Marion, St. Augustine / Iza Duffus Hardy -- St. Anne du Beaupré, Quebec / Anna T. Sadlier -- The Wadsworth-Longfellow House, Portland / Nathan Goold -- Washington\u27s Headquarters, Newburgh / Gulian C. Verplanck -- The Tabernacle, Salt Lake City / Lady Hardy -- The National Washington Monument / Joseph B. Varnum -- The Clarke-Hancock House, Lexington -- Castle St. Louis, Quebec / J. M. LeMoine -- Sunnyside, Tarrytown / Benson J. Lossing -- The Old Witch House, Salem / Esther Singleton -- Shrine of Guadalupe / Thomas Unett Brocklehurst -- Christ Church, Alexandria / Bishop Meade -- A glimpse at the houses of New Orleans / Lady Hardy -- The Chateau de Ramezay, Montreal / Major A. C. Yate -- The City Hall, New York / Arthur Shadwell Martin -- The White House -- The White House of the Confederacy, Richmond -- The Old Statehouse, Boston / Edward G. Porter -- The Morris-Jumel House, New York -- Fort Sumter / Iza Duffus Hardy -- Old Stone Tower, Newport / Benson J. Lossing -- St. Paul\u27s Chapel, New York -- Charles Hemstreet -- Faneuil Hall, Boston / Edward G. Porter -- Liberty Enlightening the World , New York / Esther Singleton. Blind stamp design on cover and spine, title emboss in gold. Cover design attributed to Alice Morse. Case binding.https://digitalcommons.risd.edu/specialcollections_books_architecture/1005/thumbnail.jp
Laboratory exercises in bacteriology and diagnostic methods.
No full textIncludes "References."In earlier editions the name of Frederick H. Billings appears first on the title-page.Mode of access: Internet
A simple method for screening bacterial colonies for mutagenized sites in plasmid DNA
No full textBecause of the multiple-step process that is involved in the detection of mutagenized restriction enzyme sites in plasmid DNA, a simple and accurate method was developed to analyse the plasmid DNA of site-directed mutagenesis experiments from bacterial colonies. The desired mutated part is located between the Eco RI restriction site on pUC19. Two mutagenic primers were designed to replace only one nucleotide on segments A and B of the bi-segmented genome of infectious bursal disease virus (IBDV). Two restriction sites were created for those mutations in each segment, Fsp I and Dra I, respectively. Following a protocol from the site-directed mutagenesis kit, the mutated plasmids were used to transform, and were propagated and maintained in DH5 alpha competent cells. Colonies were picked from the master plate, and used as DNA template for PCR. The PCR technique included the design of two pairs of primers, one for each segment, which were to amplify a region up to 1000 bp. Samples were pre-incubated for 3 min at 94 degrees C to induce bacterial lysis before starting the nucleic acid amplification. The PCR products 918 bp from segment A and 650 bp from segment B were digested with Fsp I and Dra I at 37 degrees C for 1 h. Products were resolved on 0.9% agarose gel which contained ethidium bromide. This method is simpler, faster and more accurate than the traditional method of mini-prep plasmid isolation and colony blot hybridization to identify the mutated plasmids
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