288 research outputs found
Design, implementation and effectiveness of ClassTranscribe
Get PDFClassTranscribe is an accessible online video lecture platform designed with the principles of Universal Design for Learning enriched with Closed Captions produced in real-time using Artificial Intelligence and Crowd-Sourcing, and Searchability for efficient student-directed learning.
It is an open-source, cross-platform system, built using modern web-technologies with a microservice architecture enabling scaling to thousands of users and sync with popular media sources and other lecture capture systems.
An evaluation of event data collected for a large-enrollment system programming course delivered solely using ClassTranscribe revealed that final exam performance was equal or better to semesters that were delivered by traditional physical lectures. When students were divided into four quartiles based on their grades, the analysis revealed that students who recorded more usage of ClassTranscribe performed better than their peers of the same quartile.Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2022-05-01The student, Chirantan Mahipal, accepted the attached license on 2020-05-12 at 14:25.The student, Chirantan Mahipal, submitted this Thesis for approval on 2020-05-12 at 14:34.This Thesis was approved for publication on 2020-05-13 at 10:31.DSpace SAF Submission Ingestion Package generated from Vireo submission #15359 on 2020-08-25 at 17:31:18Made available in DSpace on 2020-08-26T23:58:49Z (GMT). No. of bitstreams: 2
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Previous issue date: 2020-05-13Embargo set by: Seth Robbins for item 115805
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Integrating Lean Six Sigma and Industry 4.0
Get PDFThis chapter highlights the integration of Lean Six Sigma (LSS) and Industry 4.0 (I4.0) concepts in today’s scenario to offer complementary benefits. I4.0 has changed the way production systems function, making tools like LSS more important than ever before for continuous process improvement. Ingenious I4.0 tools support the data-driven methods employed in LSS. Furthermore, enhancing processes may help businesses make better use of cutting-edge technology, such as those used for collecting and analyzing data. Collecting and analyzing data is a cornerstone of quality improvement methods used to address existing problems with quality. Combining the principles of Lean Manufacturing with the six-sigma methodology may help to reduce process variance and, in turn, improve quality. With robust data analysis methodologies that can wring useful insights from accessible big data, I4.0 is moving in the direction of digitalizing industrial operations. It is feasible to drive meaningful choices by employing these strategies in each and every phase of LSS cycles
Analytical Strategies for Source Identification and Environmental Justice
No full textAccurate pollution source identification is essential for effective environmental regulation and sustainable resource management. This process involves determining the origin, nature, and quantity of pollutants & emerging contaminants, enabling targeted interventions, and reducing reliance on generalized assumptions. Pollution sources are majorly categorized as point or nonpoint for water pollution, mobile or stationary for air pollution, each category represent unique monitoring and certain challenges. These can be overcome by using advanced methodologies & techniques, including environmental sampling, remote sensing integrated with GIS, chemical fingerprinting, and statistical modelling, enhance source attribution by linking pollutants & emerging contaminants to their origins with high spatial and temporal resolution. However, the exact identification of sources remains difficult because of analytical constraints and imperfect data quality and overlapping processes that occur. The detection technologies need improvement and standardization in monitoring frameworks to overcome these challenges. This chapter will also explain the ability to identify pollution sources effectively enables regulator-ship based on science which leads to declassification protocols and enables protective strategies that protect both nature and public well-being. Moreover, it is also well integrated with the United Nations Sustainable Development Goals; Clean Water & Sanitation (SDG 6), Sustainable Cities and Communities (SDG 11), Responsible Consumption & Production (SDG 12), Life below Water (SDG 14), Life on Land (SDG 15), Peace, Justice & Strong Institutions (SDG 16)
고추의 C3HC4 형 RING Zinc Finger Protein의 기능 연구
Get PDF학위논문 (박사)-- 서울대학교 대학원 자연과학대학 생명과학부, 2017. 8. 홍주봉.RING finger is a cysteine-rich domain (40–60 residues) in which cysteine and histidine residues ligated with two zinc ions in a cross-brace manner to stabilize the domain structure. RING finger proteins play a crucial role in diverse biological processes such as transcriptional activation, recombination of DNA, translational processes, signal transduction, programmed cell death, membrane association, and protein folding and assembly. RING finger proteins have also been implicated in various and fundamental functions in plant growth and development. Although, RING finger proteins have been closely and repeatedly involved in the development of multiple organisms, however, cases reported in plants are comparatively limited. Capsicum annuum RING Zinc Finger Protein 1 (CaRZFP1) is a C3HC4-type RING zinc finger protein gene previously isolated from a cDNA library of heat-stressed hot pepper. Expression of CaRZFP1 was also induced by diverse abiotic stresses including cold, dehydration and high salinity in hot pepper. Transcript induction kinetics of CaRZFP1 was distinct in different plant parts of hot pepper. In our previous work elucidating in vivo function of CaRZFP1, we transferred CaRZFP1 into tobacco (Nicotiana tabacum)transgenic tobacco exhibited enhanced growth and tolerance to abiotic stresses. As further analysis of CaRZFP1 ectopic expression in a heterologous host plant, this study, I mobilized and constitutively overexpressed CaRZFP1 into lettuce (Lactuca sativa). In contrast to tobacco, transgenic lettuce exhibited poorer growth and delayed flowering compared with vector-only controlscharacteristics included weakened leaf growth, shorter plant height, and stunted root growth. Thus, ectopic expression of CaRZFP1 caused pleiotropic developmental changes in the CaRZFP1-transgenic lettuce plants. In addition, I found a significant correlation between CaRZFP1 expression and the degree of diminished growth of CaRZFP1-transgenic lettuce plants. Starting from T2, I categorized transgenic lettuce lines according to CaRZFP1 transcript expression levels (low, medium, and high). The correlation between CaRZFP1 transcript level and a negative phenotypic effect was repeatedly maintained through the next generations of CaRZFP1-transgenic lettuce plants. Overall, CaRZFP1 expression impeded the growth and development of CaRZFP1-transgenic lettuce plants in a dose-dependent fashion. The weaken growth of CaRZFP1-transgenic lettuce plants continued to the late stage of development. At full growth, CaRZFP1-transgenic lettuce was shorter than vector-only plants. CaRZFP1-transgenic lettuce delayed in flowering and inflorescence size was smaller compared to vector-only plants. Flower size did not differ significantly between the transgenic and control lines, but the former head significantly fewer flowers per inflorescence.
To examine the retarded growth of CaRZFP1-transgenic lettuce and the robust growth of CaRZFP1-transgenic tobacco at the cellular level, I analyzed leaf, stem, and root sections of both plants and compared them with vector-only controls. The cross sections of leaves and stems of CaRZFP1-transgenic lettuce and vector-only lettuce were not distinguishable in terms of morphology, cell size, or tissue organization. However, the development of endodermis and vascular bundles was significantly hampered by CaRZFP1 expression in transgenic lettuce roots. Remarkably, I observed a strong correlation, albeit negative, between the expression level of CaRZFP1 and the degree of disruption of internal root morphology. The overall root morphology and tissue patterns were preserved in CaRZFP1-transgenic tobacco roots. CaRZFP1 over-expression effect on the abiotic stress tolerance in CaRZFP1-transgenic lettuce plants was also examined, but no significant differences were observed between the CaRZFP1-transgenic lettuce and vector-only plants. To identify genes that might be involved in this phenotypic effect, transcriptome analyses on transgenic plants of both species were performed, uncovering dozens of genes that reflect the different outcomes between tobacco and lettuce. In particular, the strong negative correlation between CaRZFP1 expression and growth in lettuce helped us isolate genes most likely involved in the phenotypic differences. These included protein kinase, transcriptional factor, transporter protein, hormone and metabolism-related genes, and some unannotated genes. I separated the up- and down-regulated genes into two groups: Group 1 included genes with correlative changes in expression level among the four CaRZFP1-transgenic lettuce lines (#6, #14, #16 and #12), while Group 2 included only genes with significant expression-level changes mainly in line #12 (highest CaRZFP1 expression). To validate the transcriptome profiling results, nine genes were randomly selected for oligo-RNA blot analysis either significantly up- or down-regulated in transgenic lettuce lines. Oligo-RNA blot results showed that the trends of the differentially expressed genes were generally consistent across the two different approaches. Therefore, collectively these results showed that a gene with a specific function in one organism can yield completely different effects depending on the host species it is moved into and address concerns of unexpectedly different outcome of a gene in different genetic environments.Chapter I. Introduction 1
I.1. Role of zinc in biology 5
I.2. Zinc Finger motif 5
I.3. Zinc finger domains structure and classification. 8
I.4. Biological functions of ZFPs 12
I.4.1. ZFPs implicated in nucleic acid interactions 13
I.4.1.1. ZFPs interact with DNA 13
I.4.1.2. ZFPs interact with RNA 16
I.4.2. ZFPs interact with protein and lipid 18
I.5. RING finger proteins 20
I. 6. Aims of this study. 25
Chapter II. Materials and Methods 26
II.1.Plant material and growth condition 27
II.2. Abiotic stresses treatment 27
II.3.Generation of transgenic lettuce plants overexpressing CaRFZP1 28
II.4.RNA and DNA blot analyses 29
II.5.Phenotypic assay 31
II.6.Thermotolerance assay 32
II.7.Cold tolerance assay 32
II.8.Drought tolerance assay 32
II.9.Histological and in situ hybridization analyses 33
II.10.Transcriptome analysis 35
II.11.Multiple sequence alignment and phylogenetic analysis 36
Chapter III. Results 37
III.1.Sequence homology and phylogenetic analyses of hot pepper CaRZFP1 with other zinc finger protein genes 38
III.2.Expression of CaRZFP1 was induced widely in different tissues of hot pepper plants in response to various abiotic stresses 43
III.3.Genomic DNA blot analysis of hot pepper 46
III.4.Generating transgenic lettuce plants overexpressing CaRFZP1 48
III.5.Growth of CaRZFP1-transgenic lettuce plants were retarded 51
III.6.Root growth of CaRZFP1-transgenic lettuce plants was impeded 56
III.7.CaRZFP1-transgenic lettuce plants were shorter in full growth and flowering was delayed 58
III.8.Developmentof endodermis and vascular bundle wasimpairedinthe CaRZFP1-transgenic lettuce roots 62
III.9.CaRZFP1-transgenic lettuce plants were intolerant to abiotic stress. 71
III.10.Transcriptome profiles of CaRZFP1-transgenic lettuce plants 77
Chapter IV. Discussion 99
Conclusion 121
References 123
Abstract in Korean 145Docto
Ecological Crime Scene Investigation
No full textOperational practices between forensic science and environmental crime investigation make up ecological crime scene investigation (ECSI), which unites multiple disciplines to document and identify ecosystem violations for prosecution. These environmental offenses include the illicit disposal of hazardous substances along with forest clearance and protected animal poaching and trafficking, discharge of pollutant and emerging contaminats into air, water, and earth surface (soil) aside from leaving behind intricate physical evidence. Moreover, reconstruction of ecological crimes and tracking of their sources requires integration between forensic ecology, environmental forensics, forensic toxicology, and criminal justice principles and techniques through ECSI. Environmental forensic scientist detect identify and quantify pollutants, as well as emerging contaminants while tracing their sources through techniques, which include water and soil collection as well as satellite analysis and DNA identification and chemical substance tracing. Ecological investigations handle harmed ecosystems and species using specialized techniques and legal frameworks because they do not solely conduct investigations on human victims like traditional forensics do. The ECSI studies extended harm resulting from damage to the environment that causes damage to biodiversity and impacts human health and climate systems. The advanced scale of environmental crimes requires ECSI to serve multiple functions that advance both environmental protection and fairness. Law enforcement depends on this field, together with policy development and public awareness, because it translates intricate ecological harm into evidence for legal proceedings. Additionally, ECSI maintains its rising importance because societies worldwide are focusing on safeguarding environmental integrity. This chapter also supports United Nations Sustainable Development Goals; Climate action (SDG 13), Life on land (SDG 15), Life Below Water (SDG 14), Peace, Justice and Strong Institutions (SDG 16)
Future Directions in Environmental Forensics
No full textEnvironmental forensics is a developing field of study applying science, law, and inquiry to identify pollution sources, timing, and accountability. Environmental forensics has been developed from pollution tracking and regulatory enforcement into a complex discipline that examines difficult environmental crimes. Pollution and environmental degradation are examined via time analysis, source attribution, and pollution fingerprinting. Modern environmental forensic investigations apply both traditional and innovative approaches. Geospatial technology, biological markers, genetic and microbiological forensics provide ecological and spatial background for contamination events. Predictive modeling, pattern discovery, and data harmonization across environmental datasets are enabled by artificial intelligence, machine learning, and big data. Research is advanced by 3D environmental reconstruction, eDNA applications, satellite, and drone observation. Despite scientific developments, environmental forensics faces legal challenges, data access, and confidentiality of environmental crimes. The future of environmental forensics rests on its capacity to welcome innovation, adapt to new challenges, and link research and policy. As environmental crimes get increasingly complex and commercially motivated, environmental forensics will be more crucial than ever in ensuring responsibility and safeguarding of ecosystems
Molecular analysis of polima cytoplasmic male sterility in Brassica napus
No full textTo identify region(s) of the mitochondrial genome that might be involved in specifying the "Polima" (pol) cytoplasmic male sterility (CMS) of Brassica napus, transcripts corresponding to 14 mitochondrial genes and DNA clones representing 90% of the mitochondrial genome of Brassica campestris were analyzed in nap (male fertile), pol (male sterile) and nuclear fertility-restored pol cytoplasm plants. CMS-correlated transcriptional differences among these plants were detected only with the ATPase subunit 6 (atp6) gene. Sequence analysis of the atp6 gene regions of pol and nap mitochondrial DNAs show that rearrangements in the pol mitochondrial genome upstream of atp6 have generated a chimeric 224-codon open reading frame, designated orfJ224, that is cotranscribed with atp6. In male sterile plants, most transcripts of this region are dicistronic, comprising both orf224 and atp6 sequences. In fertility restored-plants, genes at either of two distinct nuclear restorer loci specifically alter this transcript pattern, resulting in predominantly monocistronic atp6 transcripts. The effect of the restorer locus on orf224/atp6 transcripts does not seem to be tissue or developmental stage specific. orf224 comprises a portion of the mitochondrial gene, orfB, fused to sequence of unknown origin. The pol mitochondrial genome contains an apparently functional copy of orfB. The expression of the atp6 region is developmentally regulated in pol plants such that levels of monocistronic atp6 transcripts are increased in seedlings as compared to the floral tissue. Preliminary data indicate that the chimeric gene, orf224, is expressed at the protein level in pol plants
Role of bilayer chain coupling on junction voltage in layered high temperature cuprate superconductors
No full text52-58The junction voltage as a function of hopping matrix element for CuO-chains, hopping of the single particle from bilayer to chain, Josephson like Cooper pair tunneling and other microscopic interactions that exit in bilayer high temperature cuprate superconductors has been studied. For this purpose, a tight binding model Hamiltonian that includes various intra and inter-bilayer interactions alongwith contributions of Josephson-like tunneling of Cooper pairs from bilayer of CuO2 planes to CuO-chains and vice-versa as well as single particle hopping between CuO2 bilayer to CuO-chains in the form of bilayer chain interaction within a unit cell has been considered. The situation considered here is equivalent to a Josephson’s coupled SNS junction. In the superconducting state, the CuO2 bilayer acts as superconducting electrode and CuO-chains as one dimensional metal under fully oxygenated overdoped state sandwiched between these two electrodes as in the case of YBa2Cu3O7 bilayer system. There is always a possibility of Cooper pairs to tunnel from one bilayer CuO2 planes to other bilayer via CuO-chains. Using Green’s function technique, we have obtained expressions for superconducting order parameter, carrier density and junction voltage within BCS formalism. The numerical analysis shows that in bilayer cuprates, the junction voltage depends on the interlayer pair tunneling between the CuO2 planes, hopping matrix element (tch) for chains and the hopping of single particle from bilayer to chains. Finally, we have compared our theoretical results on junction voltage with the existing experimental results
Organization of 5S ribosomal RNA genes in tea (<i>Camellia sinensis</i>)
No full textThe 5S rRNA genes in the Camellia sinensis (L.) O. Kuntze (tea) genome are arranged as tandem repeat units of 300 and 325 bps. The 2 classes of tandem repeats were discovered by Southern hybridisation of tea genomic DNA with a 5S rRNA gene PCR product.Key words: Camellia species, 5S rDNA, multigene family, tandem repeats, spacers.</jats:p
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