4,354 research outputs found
Strategic use of nickel(0)-catalyzed enyne–epoxide reductive coupling toward the synthesis of (−)-cyatha-3,12-diene
No full textVarious situations are explored in which the nickel(0)-catalyzed enyne–epoxide reductive coupling was utilized to access key intermediates toward the total synthesis of (−)-cyatha-3,12-diene (1). Enantioenriched 3,5-dien-1-ols with a variety of functionality were obtained in a straightforward manner from easily accessible 1,3-enynes and terminal epoxides.National Institute of General Medical Sciences (U.S.) (GM-063755)Massachusetts Institute of Technology. Undergraduate Research Opportunities Progra
Development of a fluorescent three‐hybrid system for the identification of protein‐protein associators
No full textThe chemically‐induced dimerization of proteins is fundamental to many key regulatory pathways. A number of natural products exert their downstream effect through the stabilization of a protein complex, either by direct binding to two distinct protein partners or via an allosteric mechanism. Here, we report a bacterial three‐hybrid system, with dual life/death and fluorescent reporters, which detects protein association and is compatible with high‐throughput screening. We use rapamycin mediated mTOR‐FKBP12 (mammalian target of rapamycin—FK506 Binding Protein 12 kDa) dimerization to validate this platform; the addition of rapamycin results in the association of these target proteins, leading to the expression of two essential life/death reporter genes and a fluorescent signal. We further used this system to quantify the activity of rapamycin by utilizing the fluorescent readout, exemplifying its potential in screening and for ranking large in vivo libraries for compounds that upregulate the association of any two given proteins
HFE C282Y homozygosity is associated with an increased risk of total hip replacement for osteoarthritis
No full textAbstract not availableYuanyuan Wang, Lyle C. Gurrin, Anita E. Wluka, Nadine A. Bertalli, Nicholas J. Osborne, Martin B. Delatycki, Graham G. Giles, Dallas R. English, John L. Hopper, Julie A. Simpson, Stephen Graves, Katrina J. Allen, Flavia M. Cicuttin
M. Patrick Graham & Steven L. McKenzie (ed.), The Chronicler as Author. Studies in Text and Texture, Sheffield, Sheffield Academic Press, 1999, (Journal for the Study of the Old Testament, Supplement Series, 263), ISBN 1-84127-057-1
No full textRobert Philippe de. M. Patrick Graham & Steven L. McKenzie (ed.), The Chronicler as Author. Studies in Text and Texture, Sheffield, Sheffield Academic Press, 1999, (Journal for the Study of the Old Testament, Supplement Series, 263), ISBN 1-84127-057-1. In: Revue d'histoire et de philosophie religieuses, 80e année n°2, Avril-juin 2000. p. 297
Ladder Polyether Synthesis via Epoxide-Opening Cascades Directed by a Disappearing Trimethylsilyl Group
No full textEpoxide-opening cascades offer the potential to construct complex polyether natural products expeditiously and in a manner that emulates the biogenesis proposed for these compounds. Herein we provide a full account of our development of a strategy that addresses several important challenges of such cascades. The centerpiece of the method is a trimethylsilyl (SiMe[subscript 3]) group that serves several purposes and leaves no trace of itself by the time the cascade has come to an end. The main function of the SiMe[subscript 3] group is to dictate the regioselectivity of epoxide opening. This strategy is the only general method of effecting endo-selective cascades under basic conditions.Johnson & Johnson (Fellowship)Boehringer Ingelheim Pharmaceuticals (Fellowship)US-UK Fulbright Commissio
The DSM diagnostic criteria for Female Sexual Arousal Disorder
No full textThis article reviews and critiques the DSM-IV-TR diagnostic criteria for Female Sexual Arousal Disorder (FSAD). An overview of how the diagnostic criteria for FSAD have evolved over previous editions of the DSM is presented and research on prevalence and etiology of FSAD is briefly reviewed. Problems with the essential feature of the DSM-IV-TR diagnosis — “an inability to attain, or to maintain…an adequate lubrication-swelling response of sexual excitement” — are identified. The significant overlap between “arousal” and “desire” disorders is highlighted. Finally, specific recommendations for revision of the criteria for DSM-V are made, including use of a polythetic approach to the diagnosis and the addition of duration and severity criteria
Still room for improvement? The educational experiences of looked after children in Scotland
No full textIn this chapter Kirstie Maclean and Graham Connelly present an analysis of joined-up thinking in Scotland from both social services and education perspectives
The DSM diagnostic criteria for female orgasmic disorder
No full textThis is the post-print version of the article. The official published version can be found at the link below.This article reviews the DSM diagnostic criteria for Female Orgasmic Disorder (FOD). Following an overview of the concept of female orgasm, research on the prevalence and associated features of FOD is briefly reviewed. Specific aspects of the DSM-IV-TR criteria for FOD are critically reviewed and key issues that should be considered for DSM-V are discussed. The DSM-IV-TR text on FOD focused on the physiological changes that may (or may not) accompany orgasm in women; one of the major recommendations here is that greater emphasis be given to the subjective aspects of the experience of orgasm. Additional specific recommendations are made for revision of diagnostic criteria, including the use of minimum severity and duration criteria, and better acknowledgment of the crucial role of relationship factors in FOD
Technical standards for respiratory oscillometry : test loads for calibration and verification
No full textIt has been brought to our attention that there is ambiguity in the ERS technical standards for respiratory oscillometry, specifically regarding the use of test loads for calibration and verification. Here it is worth making a distinction between recommendations for manufacturers versus users. We recognise that a standardised reactive test load has not been established for calibration or verification. This is an active research area, driven by the poor comparability in reactance which remains across devices
Asexuality: Classification and characterization
No full textThis is a post-print version of the article. The official published version can be obtaineed at the link below.The term “asexual” has been defined in many different ways and asexuality has received very little research attention. In a small qualitative study (N = 4), individuals who self-identified as asexual were interviewed to help formulate hypotheses for a larger study. The second larger study was an online survey drawn from a convenience sample designed to better characterize asexuality and to test predictors of asexual identity. A convenience sample of 1,146 individuals (N = 41 self-identified asexual) completed online questionnaires assessing sexual history, sexual inhibition and excitation, sexual desire, and an open-response questionnaire concerning asexual identity. Asexuals reported significantly less desire for sex with a partner, lower sexual arousability, and lower sexual excitation but did not differ consistently from non-asexuals in their sexual inhibition scores or their desire to masturbate. Content analyses supported the idea that low sexual desire is the primary feature predicting asexual identity
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