100,381 research outputs found
Comportamento della fosfatasi acida e della beta-glucuronidasi in organi di pulcini trattati con rRNA da toprina BP
No full textFasciola hepatica: increase of glycogen-phosphorylase activity due to prostaglandins
No full textBoth prostaglandin E1 (PGE1) and prostaglandin F2α (PGF2α) stimulate the glycogen phosphorylase (EC 2.4.1.1.) activity of Fasciola hepatica. Whole or sliced parasites were incubated with PGE1 (2.8 × 10-7 and 2.8 × 10-5M) and PGF2α(2.1 × 10-7 and 2.1 × 10-5 M) and enzyme activity was measured in homogenates prepared immediately following the incubation. No substantially different effect was noted between the two assayed doses of prostaglandins. Prostaglandins appeared to be less effective in sliced parasites
Cyclic AMP phosphodiesterase activity in Fasciola hepatica
No full textThe authors studied the behaviour of cyclic 3',5' AMP phosphodiesterase in the 2,000 g supernatant of the F. hepatica homogenate, under basal conditions and after addition of various substances. Dopamine markedly inhibits the enzyme activity, imidazole causes a strong activation, while serotonin, theophylline, PGE1, and PGF(2α) appear to be ineffective
A putative protein structurally related to zygote arrest 1 (Zar1), Zar1-like, is encoded by a novel gene conserved in the vertebrate lineage
No full textIdentification and characterization of a bovine cDNA and the corresponding gene coding for a novel protein structurally related to Zar1, therefore called Zar1-like, are here reported for the first time. Structure of Zar1-like is similar to Zar1 gene, nevertheless they are located on distinct chromosomes. We demonstrated that the new gene as well as its genomic context are conserved along the whole vertebrate lineage. Analysis of the deduced protein primary structure showed a high conservation, among vertebrates, of the C-terminal region, where the putative presence of both zinc finger motifs and classical nuclear localization signals is also shared with Zar1. Bovine Zar1-like and the only two other available mRNA leader sequences (human and chicken) exhibit a number of upstream AUGs, suggesting that they are likely to be regulated at translational level. Expression patterns of the cattle transcripts show that Zar1-like is absent in early stages of embryo development, whereas Zar1 is expressed in matured oocytes and in in vitro produced pre-implantation embryos. In adult tissues Zar1-like transcript expression appears to be less restricted than Zar1, nevertheless, at least in bovine, both mRNAs are co-expressed in gonads, raising the question of a possible functional link
Ciclic AMP involvement in "in vitro" lysosomal enzyme release from the scraped intestinal mucosa of rat
No full textLiver glycogen-phosphorylase activity in rats after intraperitoneal implanctation of Fasciola hepatica
No full textInfluence of age and cortisone treatment on two intestinal lysosomal hydrolases in young rats
No full textThe effects of cortisone acetate injected i.p. on acid phosphatase and beta-glucuronidase in the small intestine mucosa of two groups of rats aging 12-14 and 20-22 days are reported. The following results are obtained: a) Total and free activities of the two enzymes show age-dependent differences. b) Cortisone acetate causes a very marked decrease of the enzymic activities only in the younger rats while being ineffective on the older ones. c) The kinetics of the enzyme release at 37 degrees C appear independent either of age or of cortisone injection. d) The "in vitro" lysosome labilizing treatments are ineffective
Molecular cloning of the cDNA coding for Xenopus laevis prion protein
No full textAbstractIsolation and characterization of the cDNA coding for the 216-residue Xenopus laevis prion protein is reported. Existence of this protein in amphibians was suggested by an EST fragment (accession number BG813008), while a conclusive demonstration is presented here. This protein exhibits a higher identity level to avian and turtle prion (more than 44%) than to mammalian prion (about 28%). Although most of the structural motifs common to known prion proteins are conserved in X. laevis, the lack of repeats represents a substantial difference. Other features worth noting are the presence of not perfectly conserved hydrophobic stretch, which is considered the prion signature, as well as the complete absence of histidine residues
Stereospecificity of pig kidney and pea seedling diamino oxidases on 2-methyl-1,4-diaminobutane
No full textDiamine oxidase from pea seedlings (PDAO) oxidizes both (R)- and (S)-2-methylputrescine at the less hindered C-4, whereas pig-kidney diamine oxidase (KDAO) shows a dependence on the stereochemistry of the substrate, since the (R)-isomer is oxidized at C-1 and the (S)-isomer at the less hindered amino group at C-4
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