23 research outputs found
RabAnalyser: a computational framework to explore the cell-to-cell variability of Rab GTPases organization
No full textL'abstract è presente nell'allegato / the abstract is in the attachmen
microRNA-Mediated Encoding and Decoding of Time-Dependent Signals in Tumorigenesis
No full textmicroRNAs, pivotal post-transcriptional regulators of gene expression, in the past decades have caught the attention of researchers for their involvement in different biological processes, ranging from cell development to cancer. Although lots of effort has been devoted to elucidate the topological features and the equilibrium properties of microRNA-mediated motifs, little is known about how the information encoded in frequency, amplitude, duration, and other features of their regulatory signals can affect the resulting gene expression patterns. Here, we review the current knowledge about microRNA-mediated gene regulatory networks characterized by time-dependent input signals, such as pulses, transient inputs, and oscillations. First, we identify the general characteristic of the main motifs underlying temporal patterns. Then, we analyze their impact on two commonly studied oncogenic networks, showing how their dysfunction can lead to tumorigenesis
Uncertainty and the politics of employment protection
No full textThis paper investigates social preferences over employment protection regulation in a general equilibrium model of dynamic labor demand and sheds some light on the comparative dynamics of Eurosclerosis. When firing costs are low, a transition to a rigid labor market is favored by all the employed workers with idiosyncratic productivity below some threshold; when their status quo level is high, preserving a rigid labor market is favored only by the employed with intermediate productivity. A more volatile environment and a lower rate of productivity growth increase the political support for labor market rigidity only in high-rents economies
Improving the performances of NafionTM-based membranes for microbial fuel cells with silica-based, organically-functionalized mesostructured fillers
No full textFlexible deposition of TiO2 electrodes for photocatalytic applications: Modulation of the crystal phase as a function of the layer thickness
No full textTiO2 thin films for electrochemical applications (e.g.; photocatalysis) may require a careful control of the crystalline phase and the surface morphology. Flexible radiofrequency magnetron sputtering deposition of TiO2 thin films (anatase, rutile or variable mixtures of the two polymorphs) onto Ti disks has been achieved by controlling the layer thickness at variable temperatures. This result is of great importance in view of the preparation of TiO2 electrodes for photo-catalytic applications. In principle, it allows to modulate the phases present in the active layer by changing the layer thickness even using different deposition temperatures, which also implies modification of the morphology of the layer, therefore addressing two major issues in the field of photocatalysis mediated by titanium dioxide
Analysis of the jurisprudence of the electoral court on the registration of political parties
No full textHistóricamente, la Corte Electoral tuvo un procedimiento reglamentario pa‐
ra la inscripción de nuevos partidos políticos. La Constitución uruguaya a partir de 1997
prevé que para que los partidos políticos puedan participar en el ciclo electoral (elecciones
nacionales, departamentales y municipales), es necesario que realicen elecciones internas
primarias, simultáneas y obligatorias para dichos partidos. La Ley N° 18.485 de 11 de mayo de 2009, en los artículos 7° a 9° establece la forma de cómo deben constituirse los partidos políticos, qué documentos deben presentar los solicitantes, así como los plazos para interponer objeciones y el procedimiento ante la Corte
Electoral. La Corte Electoral desde el año 2010 a la fecha ha recibido solicitudes de inscripción de nuevos partidos políticos, dictando para ello la reglamentación de los artículos señalados. Por Circular N° 8894 de 13 de noviembre de 2012, se estableció que las solicitudes de los partidos políticos que quieran participar en el período electoral siguiente, deben ser presentadas 150 días antes de la fecha de las elecciones internas. Hasta el año 2019 y desde el año 2012 se han presentado alrededor de quince solicitudes de inscripción de nuevos partidos políticos en aplicación de la ley señalada. No todas las solicitudes han culminado con la inscripción del partido político, siendo objetadas por particulares, por otros partidos o por la propia Corte Electoral. El autor se propone exponer el procedimiento para la inscripción de partidos políticos, la ley y su reglamentación vigente, y analizar cada una de las resoluciones de la Corte Electoral desde 2010 hasta el año 2020. Se realiza un estudio sistematizado de cada requisito que establece la normativa para poder constituir un partido político: el nombre, el estatuto, la carta orgánica, el programa de principios, las firmas requeridas y las autoridades provisorias. Los partidos políticos en tanto actores necesarios del sistema electoral uruguayo, también han participado en los procedimientos de inscripción oponiéndose y objetando el registro de otros partidos.Historically, the Electoral Court had a regulatory procedure for the registration of new political parties. The Uruguayan Constitution from 1997 provides that in order for political parties to participate in the electoral cycle (national, departmental and municipal elections), it is necessary that they hold primary, simultaneous and mandatory internal elections for said parties. Law No. 183485 of May 11, 2009, in articles 7 to 9 establishes the way in which political parties must be constituted, what documents applicants must present, as well as the
deadlines for filing objections and the procedure before the Electoral Court. The Electoral Court from 2010 to date has received applications for registration of new political parties, dictating for this the regulations of the indicated articles. By Circular No. 8894 of November 13, 2012, it was established that requests from political parties that want to participate in the following electoral period must be submitted 150 days before the date of the internal elections. Until 2019 and since 2012, around fifteen applications for the registration of new political parties have been submitted in application of the aforementioned law. Not all applications have culminated in the registration of the political party, being objected by individuals, by other parties or by the Electoral Court itself. The author intends to expose the procedure for the registration of political parties, the law and its current regulations, and analyze each of the resolutions of the Electoral Court from 2010 to 2020. A systematic study of each requirement established by the regulations to be able to constitute a political party: the name, the statute, the organic charter, the program of principles, the required signatures and the provisional authorities. Political parties, as necessary actors of the Uruguayan electoral system, have also participated in the registration procedures, opposing and objecting to the registration of other parties.Historicamente, a Justiça Eleitoral tinha um procedimento regulatório para o registro de novos partidos políticos. A Constituição uruguaia de 1997 dispõe que para que os partidos políticos participem do ciclo eleitoral (eleições nacionais, departamentais e municipais) é necessário que realizem eleições internas primárias, simultâneas e obrigatórias para esses partidos. A Lei nº 18.485, de 11 de maio de 2009, nos artigos 7º a 9º estabelece a forma como os partidos políticos devem ser constituídos, os documentos que os candidatos devem apresentar, bem como os prazos para impugnação e o procedimento na Justiça Eleitoral. Desde 2010, a Justiça Eleitoral recebe pedidos de registro de novos partidos políticos, ditando a regulamentação dos artigos citados. Pela Circular nº 8.894, de 13 de novembro de 2012, ficou estabelecido que as solicitações dos partidos políticos que desejam participar do período eleitoral seguinte devem ser apresentadas 150 dias antes da data das eleições internas. Até 2019 e desde 2012, foram apresentados cerca de quinze pedidos de registo de novos partidos políticos em aplicação da referida lei. Nem todos os pedidos culminaram no registro do partido político, sendo contestados
por pessoas físicas, por outros partidos ou pela própria Justiça Eleitoral. O autor pretende expor o procedimento de registro de partidos políticos, a lei e seu regulamento em vigor, e analisar cada uma das resoluções da Justiça Eleitoral de 2010 a 2020. Um estudo sistematizado de cada requisito estabelecido pelo regulamento para poder
Constituir partido político: o nome, o estatuto, a carta orgânica, o programa de princípios, as assinaturas exigidas e as autoridades provisórias. Os partidos políticos, como atores necessários do sistema eleitoral uruguaio, também
participaram dos procedimentos de registro, opondose e objetando ao registro de outros
partidos
A Bioinspired Cownose Ray Robot for Seabed Exploration
No full textThis article presents the design and the experimental tests of a bioinspired robot mimicking the cownose ray. These fish swim by moving their large and flat pectoral fins, creating a wave that pushes backward the surrounding water so that the fish is propelled forward due to momentum conservation. The robot inspired by these animals has a rigid central body, housing motors, batteries, and electronics, and flexible pectoral fins made of silicone rubber. Each of them is actuated by a servomotor driving a link inside the leading edge, and the traveling wave is reproduced thanks to the flexibility of the fin itself. In addition to the pectoral fins, two small rigid caudal fins are present to improve the robot’s maneuverability. The robot has been designed, built, and tested underwater, and the experiments have shown that the locomotion principle is valid and that the robot is able to swim forward, perform left and right turns, and do floating or diving maneuvers
Eleiçoes em tempo de pandemia no Uruguai : entre realidade e a letra da constituiçao
No full textEn el presente artículo el autor se propone analizar cómo incidió la pande mia mundial de COVID-19 surgida a principios de 2020, en la celebración de las elecciones departamentales y municipales previstas constitucionalmente para mayo. La suspensión de las elecciones y su prórroga mediante una solución legislativa, que contó con el consenso de todos los partidos políticos con representación parlamentaria. Esa solución
legislativa, a todas luces, inconstitucional, constituye un antecedente de modificación de la Constitución de forma consensuada y consciente por todos los actores políticos del Uruguay, que debe ser analizado a la luz del Estado constitucional de Derecho y del Derecho Electoral.Throughout this paper the author intends to review the impact of the COVID-19 global pandemic emerged in early 2020 on the local and municipal governments elections constitutionally provided for to be held in May. The suspension of such elections and extension thereof by means of a legislative solution was endorsed by all political
parties with parliamentary representation. This legislative solution, strikingly unconstitutional, portrays a precedent of an agreed and conscious Constitutional amendment by all political stakeholders in Uruguay that must be analyzed in the light of the constitutional Rule of Law and Electoral Law.No presente artigo o autor propõe-se analizar como incidiu a pandemia mundial do COVID-19 surgida no começo do ano 2020, na celebração das eleições departamentais e municipais previstas no texto constitucional para o mês de maio. A suspensãdas eleições e sua prorrogação através de uma solução legislativa, que contou com consenso de todos os partidos políticos com representação parlamentar. Essa solução legislativa, claramente, inconstitucional, constitui um antecedente de modificação da Constituição de maneira consensuada e consciente por todos os atores políticos do Uruguai, o que deve ser analisado à luz do Estado constitucional de Direito e do Direito Eleitora
Discovery of a Small Molecule with an Inhibitory Role for RAB11
No full textRAB11, a pivotal RabGTPase, regulates essential cellular processes such as endocytic recycling, exocytosis, and autophagy. The protein was implicated in various human diseases, including cancer, neurodegenerative disorders, viral infections, and podocytopathies. However, a small-molecular inhibitor is lacking. The complexity and workload associated with potential assays make conducting large-scale screening for RAB11 challenging. We employed a tiered approach for drug discovery, utilizing deep learning-based computational screening to preselect compounds targeting a specific pocket of RAB11 protein with experimental validation by an in vitro platform reflecting RAB11 activity through the exocytosis of GFP. Further validation included the exposure of Drosophila by drug feeding. In silico pre-screening identified 94 candidates, of which 9 were confirmed using our in vitro platform for Rab11 activity. Focusing on compounds with high potency, we assessed autophagy, which independently requires RAB11, and validated three of these compounds. We further analyzed the dose–response relationship, observing a biphasic, potentially hormetic effect. Two candidate compounds specifically caused a shift in Rab11 vesicles to the cell periphery, without significant impact on Rab5 or Rab7. Drosophila larvae exposed to another candidate compound with predicted oral bioavailability exhibited minimal toxicity, subcellular dispersal of endogenous Rab11, and a decrease in RAB11-dependent nephrocyte function, further supporting an inhibitory role. Taken together, the combination of computational screening and experimental validation allowed the identification of small molecules that modify the function of Rab11. This discovery may further open avenues for treating RAB11-associated disorders
