206 research outputs found
Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines
No full textBackground. Fruit coloration of red-skinned grapevines is mainly due to anthocyanin pigments. We analysed a panel of nine cultivars that included extreme phenotypes for berry colour, ranging from green (absence of anthocyanins) to red, purple, violet and blue. Expression of six genes of the anthocyanin pathway coding for flavanone-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), flavonoid 3',5'-hydroxylase (F3'5'H), UDP-glucose:flavonoid-3-O-glucosyltransferase (UFGT), glutathione-S-transferase (GST), O-methyltransferase (OMT) and four transcription factors (MybA, MybB, MybC, MybD) was analysed by quantitative RT-PCR at four developmental stages from before the onset of ripening until full maturity and compared to anthocyanin metabolites. Results. Total anthocyanin content at full maturity correlated well with the cumulative expression of F3H, UFGT and GST throughout ripening. Transcripts of the last two genes were absent in the green-skinned cultivar 'Sauvignonasse', also known as 'Tocai friulano', and were at least 10-fold less abundant in pale red cultivars, such as 'Pinot gris' and 'Gewürztraminer', compared to fully coloured cultivars. Predominance of tri-hydroxylated anthocyanins (delphinidin, petunidin and malvidin) in cultivars bearing dark berries with violet and blue hue was associated with higher ratios of F3'5'H/F3'H transcription, compared to red-skinned cultivars. Higher levels of OMT transcripts were observed in berries of cultivars that accumulated methoxylated forms of anthocyanins more abundantly than non-methoxylated forms. Conclusion. Colour variation of the grape berry conforms to a peculiar pattern of genotype-specific expression of the whole set of anthocyanin genes in a direct transcript-metabolite-phenotype relationship. Cumulative mRNA levels of the structural genes and their relative abundance throughout ripening explained per se the final phenotype for anthocyanin content, anthocyanin composition, colour intensity and colour hue of grapes at berry maturity. © 2007 Castellarin and Di Gaspero; licensee BioMed Central Ltd
Fruit ripening in Vitis vinifera L.: An overview of the relationships among hormones, transcripts, and metabolites
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Effetto dello stress idrico sulla biosintesi dei composti fenolici nella bacca di Vitis vinifera L. cv 'Merlot'
No full textPreveraison water deficit accelerates berry color change in merlot grapevines
No full textIn red varieties, berry color change from green to red is one of the first events associated with ripening and is often used as an indicator of veraison by viticulturists. Water deficit can accelerate ripening and increase pigment accumulation in the berry skin. The impact of water deficit on the timing and progression of berry color change in the vineyard has been largely overlooked. Here we present three years of observations (2011 to 2013) on the progression of color change in Merlot vines subjected to water deficit (WD) or irrigation (C) regimes. Water deficit did not affect the date at which berries started changing color in 2011 and 2012, but pigmentation began three days earlier in WD than in C vines in 2013. Water deficit accelerated pigmentation in all three years and WD berries completed color change five days earlier than C berries on average. © 2016 by the American Society for Enology and Viticulture
Expression of flavonoid genes in the red grape berry of 'Alicante Bouschet' varies with the histological distribution of anthocyanins and their chemical composition
No full textBerry phenolics of grapevine under challenging environments
No full textPlant phenolics have been for many years a theme of major scientific and applied interest. Grape berry phenolics contribute to organoleptic properties, color and protection against environmental challenges. Climate change has already caused significant warming in most grape-growing areas of the world, and the climatic conditions determine, to a large degree, the grape varieties that can be cultivated as well as wine quality. In particular, heat, drought and light/UV intensity severely affect phenolic metabolism and, thus, grape composition and development. In the variety Chardonnay, water stress increases the content of flavonols and decreases the expression of genes involved in biosynthesis of stilbene precursors. Also, polyphenolic profile is greatly dependent on genotype and environmental interactions. This review deals with the diversity and biosynthesis of phenolic compounds in the grape berry, from a general overview to a more detailed level, where the influence of environmental challenges on key phenolic metabolism pathways is approached. The full understanding of how and when specific phenolic compounds accumulate in the berry, and how the varietal grape berry metabolism responds to the environment is of utmost importance to adjust agricultural practices and thus, modify wine profile. © 2013 by the authors; licensee MDPI, Basel, Switzerland
Water deficit enhances anthocyanin accumulation in berry skin through up-regulation of genes of the biosynthetic pathway
No full textImpact of water deficit on the synthesis of phenolic compounds during berry ripening of Vitis vinifera cv. Merlot
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