36 research outputs found
Author Correction: Six-pack holography for dynamic profiling of thick and extended objects by simultaneous three-wavelength phase unwrapping with doubled field of view
No full textSix-pack holography and dynamic synthetic aperture superresolution (Conference Presentation)
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Six-pack holography for dynamic profiling of thick and extended objects by simultaneous three-wavelength phase unwrapping with doubled field of view
No full textAbstract Dynamic holographic profiling of thick samples is limited due to the reduced field of view (FOV) of off-axis holography. We present an improved six-pack holography system for the simultaneous acquisition of six complex wavefronts in a single camera exposure from two fields of view (FOVs) and three wavelengths, for quantitative phase unwrapping of thick and extended transparent objects. By dynamically generating three synthetic wavelength quantitative phase maps for each of the two FOVs, with the longest wavelength being 6207 nm, hierarchical phase unwrapping can be used to reduce noise while maintaining the improvements in the 2π phase ambiguity due to the longer synthetic wavelength. The system was tested on a 7 μm tall PDMS microchannel and is shown to produce quantitative phase maps with 96% accuracy, while the hierarchical unwrapping reduces noise by 93%. A monolayer of live onion epidermal tissue was also successfully scanned, demonstrating the potential of the system to dynamically decrease scanning time of optically thick and extended samples
Dynamic Tomographic Phase Microscopy by Double Six-Pack Holography
No full textThree-dimensional
(3D) optical imaging of rapidly moving biological
cells is difficult to achieve as such samples cannot be scanned over
time. Here, we present a dynamic scan-free optical tomography approach
for stain-free 3D imaging of biological cells using our new double
six-pack tomography technique, whereby 12 off-axis holograms are captured
in a single camera exposure without sacrificing resolution or field
of view. The proposed system illuminates the sample from 12 angles
simultaneously, and 3D refractive index (RI) tomograms are reconstructed
from each recorded video frame of the dynamic sample. The technique
is verified experimentally by recording flowing silica beads, 3 μm
in diameter, with the resulting tomogram RI accuracy being 98.5%.
A live swimming sperm cell is also imaged, and dynamic 3D imaging
results for both beads and sperm cell are presented. The proposed
technique represents a 12-fold increase in dynamic holographic data
for tomography
Automated analysis of individual sperm cells using stain-free interferometric phase microscopy and machine learning
No full textStain-free interferometric phase microscopy correlation with DNA fragmentation stain in human spermatozoa
No full textDynamic Tomographic Phase Microscopy by Double Six-Pack Holography
No full textThree-dimensional
(3D) optical imaging of rapidly moving biological
cells is difficult to achieve as such samples cannot be scanned over
time. Here, we present a dynamic scan-free optical tomography approach
for stain-free 3D imaging of biological cells using our new double
six-pack tomography technique, whereby 12 off-axis holograms are captured
in a single camera exposure without sacrificing resolution or field
of view. The proposed system illuminates the sample from 12 angles
simultaneously, and 3D refractive index (RI) tomograms are reconstructed
from each recorded video frame of the dynamic sample. The technique
is verified experimentally by recording flowing silica beads, 3 μm
in diameter, with the resulting tomogram RI accuracy being 98.5%.
A live swimming sperm cell is also imaged, and dynamic 3D imaging
results for both beads and sperm cell are presented. The proposed
technique represents a 12-fold increase in dynamic holographic data
for tomography
Dynamic Tomographic Phase Microscopy by Double Six-Pack Holography
No full textThree-dimensional
(3D) optical imaging of rapidly moving biological
cells is difficult to achieve as such samples cannot be scanned over
time. Here, we present a dynamic scan-free optical tomography approach
for stain-free 3D imaging of biological cells using our new double
six-pack tomography technique, whereby 12 off-axis holograms are captured
in a single camera exposure without sacrificing resolution or field
of view. The proposed system illuminates the sample from 12 angles
simultaneously, and 3D refractive index (RI) tomograms are reconstructed
from each recorded video frame of the dynamic sample. The technique
is verified experimentally by recording flowing silica beads, 3 μm
in diameter, with the resulting tomogram RI accuracy being 98.5%.
A live swimming sperm cell is also imaged, and dynamic 3D imaging
results for both beads and sperm cell are presented. The proposed
technique represents a 12-fold increase in dynamic holographic data
for tomography
Individual sperm selection by microfluidics integrated with interferometric phase microscopy
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