213,278 research outputs found
Silver City Public Library Collection; no.05280
No full textSepia image of Hanover- a small mining town situated in a valley at the foot of an arid scrub covered mountainous terrain outside of Silver City. Stamped on the lower left corner of the original negative; ""Hermosa Copper Co."" Stamped on the lower right corner of the original negative; ""Hanover N. M."" Image mounted on a dark gray matte board.Master file: image/tiff; 124,455 KB; Computer Hardware: Intel Pentium (R) 4 3.20 GHz/ 1.99 GB RAM manufactured by Dell; Operating system: Windows XP 2002; Creation software: Adobe Photoshop CS2 version 9.0.2; Scanner: flatbed reflective scanner Microtek 1000XL; Scanner software: Microtek SilverFast Ai 6.4.2r2b; Scanned by Jackie Becker on 2009-10-15
Silver City Chamber of Commerce Collection; no.05787
No full textBlack and white image of a large number of cattle walking through a dry river bed near Mimbres, N. M. Two unidentified cowboys are seen herding the cattle. A few buildings can be seen at left. Verso: Stamped in purple ink; ""Melvin H Porterfild, Silver City, NM.""Master file: image/tiff; 112,503 KB; Computer Hardware: Intel Pentium (R) 4 3.20 GHz/ 1.99 GB RAM manufactured by Dell; Operating system: Windows XP 2002; Creation software: Adobe Photoshop CS2 version 9.0.2; Scanner: flatbed reflective scanner Microtek 1000XL; Scanner software: Microtek SilverFast Ai 6.4.2r2b; Scanned by Jackie Becker on 2009-10-15
Silver City- Grant County Chamber of Commerce Collection; no.02056
No full textBlack and white image of two unidentified children standing and looking at a large formation of rocks, location at the City of Rocks. A late 30's or early 40's era automobile is parked in front of the rocks. Verso: Typed in black ink; ""Located a short distance from Silver City off the old Butterfield trail (now U. S. ## 260). Park consists of 640 acres and consists of weird rock formations. Picnic facilities are available."" Non-mounted image.Master file: image/tiff; 111,815 KB: Computer Hardware: Intel Pentium (R) 4 3.20 GHz/ 1.99 GB RAM manufactured by Dell; Operating system: Windows XP 2002; Creation software: Adobe Photoshop CS2 version 9.0.2; Scanner: flatbed reflective scanner Microtek 1000XL; Scanner software: Microtek SilverFast Ai 6.4.2r2b; Scanned by Jackie Becker on 2010-02-22
Silver City- Grant County Chamber of Commerce Collection; no.01673
No full textBlack and white image of six unidentified children mingling outside the family civilian quarters at Fort Bayard, New Mexico. Non-mounted image.Master file: image/tiff; 107,749 KB: Computer Hardware: Intel Pentium (R) 4 3.20 GHz/ 1.99 GB RAM manufactured by Dell; Operating system: Windows XP 2002; Creation software: Adobe Photoshop CS2 version 9.0.2; Scanner: flatbed reflective scanner Microtek 1000XL; Scanner software: Microtek SilverFast Ai 6.4.2r2b; Scanned by Jackie Becker on 2010-02-26
Silver City- Grant County Chamber of Commerce Collection; no.01674
No full textBlack and white image of four unidentified children posed standing in front of a large tent structure possibly at Fort Bayard, New Mexico. Non-mounted image.Master file: image/tiff; 103,275 KB: Computer Hardware: Intel Pentium (R) 4 3.20 GHz/ 1.99 GB RAM manufactured by Dell; Operating system: Windows XP 2002; Creation software: Adobe Photoshop CS2 version 9.0.2; Scanner: flatbed reflective scanner Microtek 1000XL; Scanner software: Microtek SilverFast Ai 6.4.2r2b; Scanned by Jackie Becker on 2010-02-26
Silver City- Grant County Chamber of Commerce Collection; no.01675
No full textBlack and white image of three unidentified children posed sitting and a goat in front of a large tent structure possibly at Fort Bayard, New Mexico. Non-mounted image.Master file: image/tiff; 106,598 KB: Computer Hardware: Intel Pentium (R) 4 3.20 GHz/ 1.99 GB RAM manufactured by Dell; Operating system: Windows XP 2002; Creation software: Adobe Photoshop CS2 version 9.0.2; Scanner: flatbed reflective scanner Microtek 1000XL; Scanner software: Microtek SilverFast Ai 6.4.2r2b; Scanned by Jackie Becker on 2010-02-26
Persistence of recombinant bacteria to antimicrobial silver
No full textSilver, owing to its effective antimicrobial properties, has been used against a broad range of microorganisms. Silver is now utilized commonly in numerous consumer products, medical devices and clinical applications. However, the mechanism of action of the silver is not yet fully established and well-understood. In addition, it is also important to understand the biochemical and evolutionary pathways that give rise to resistance. Here, we report new genetic determinants for silver resistance in E. coli and explore aspects of their mechanism and laboratory evolution.
Initial exploration of the antimicrobial activity of silver showed that (1) antimicrobial ability of silver is time and dose-dependent; (2) Ag ions have much more antibiotic activity than silver nanoparticles (AgNPs) and (3) the antimicrobial ability of AgNPs is size-dependent. Further selection for resistance genes of E. coli using AgNO3 and AgNPs led to the identification of several candidates, including cysD and ycdB, which displayed cross-resistance to Ag ion and AgNPs as well as Cu+ and Cd2+. The genes cysD and ycdB conferred less resistance to metallic Ag(0) under anaerobic incubation than aerobic incubation. These results support that Ag+ ions are the main toxic agents of AgNPs. These novel anti-silver genes also endowed resistance to the antibiotics kanamycin and ampicillin; in these experiments, antibacterial synergy between kanamycin and silver, but not between ampicillin and silver, was also found. Quantification of oxygen radicals suggest that silver ion is bactericidal through production of reactive oxygen species and that silver-resistance genes prevent their generation.
The selected gene ycdB and control gene cueO, both of which led to increased silver resistance, encode Tat-dependent proteins, which are transported after folding from cytoplasm to periplasm. Chapter 2 focuses on several Tat-containing genes, which also gave more resistance to Ag ion. The 7 selected Tat sequence genes, including torA, yedY, sufI, ycdO and hybA, were recombinantly expressed in various truncated forms, showing that for ycdB and yedY deleting Tat sequences impaired export and silver-resistance ability, despite increased expression, but that for other Tat genes deleting Tat had little effect on either periplasmic translocation or resistance. In all cases, expression of the Tat export sequence alone or with the his-tag in absence of the gene led to suppression of resistance.
Finally, we explored the evolvability of selected genes, such as yeaO, ydgT, iscA and ycdB for silver-resistance. Evolved mutants of yeaO and ydgT were found that endowed increased resistance to silver compared to wildtypes. In these two cases, increased resistance to silver did not lead to increased antibiotic resistance. In short, several kinds of anti-silver genes were identified in our studies, showing various pathways rendering resistance to silver. Weak resistance functions for some genes were evolvable. Our studies provide a deeper insight into silver’s mechanism of action and of the possible resistance pathways in bacteria, which may in some cases lead also to cross-resistance to antibiotics
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Inactivation of the antibacterial and cytotoxic properties of silver ions by biologically relevant compounds
No full textThere has been a recent surge in the use of silver as an antimicrobial agent in a wide range of domestic and clinical products, intended to prevent or treat bacterial infections and reduce bacterial colonization of surfaces. It has been reported that the antibacterial and cytotoxic properties of silver are affected by the assay conditions, particularly the type of growth media used in vitro. The toxicity of Ag+ to bacterial cells is comparable to that of human cells. We demonstrate that biologically relevant compounds such as glutathione, cysteine and human blood components significantly reduce the toxicity of silver ions to clinically relevant pathogenic bacteria and primary human dermal fibroblasts (skin cells). Bacteria are able to grow normally in the presence of silver nitrate at >20-fold the minimum inhibitory concentration (MIC) if Ag+ and thiols are added in a 1:1 ratio because the reaction of Ag+ with extracellular thiols prevents silver ions from interacting with cells. Extracellular thiols and human serum also significantly reduce the antimicrobial activity of silver wound dressings Aquacel-Ag (Convatec) and Acticoat (Smith & Nephew) to Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli in vitro. These results have important implications for the deployment of silver as an antimicrobial agent in environments exposed to biological tissue or secretions. Significant amounts of money and effort have been directed at the development of silver-coated medical devices (e.g. dressings, catheters, implants). We believe our findings are essential for the effective design and testing of antimicrobial silver coatings
Formation of nanoscale elemental silver particles via enzymatic reduction by Geobacter sulfurreducens
No full textGeobacter sulfurreducens reduced Ag(I) (as insoluble AgCl or Ag+ ions), via a mechanism involving c-type cytochromes, precipitating extracellular nanoscale Ag(0). These results extend the range of metals known to be reduced by Geobacter species and offer a method for recovering silver from contaminated water as potentially useful silver nanoparticles
Effect of silver content on the structure and antibacterial activity of silver-doped phosphate-based glasses
No full textStaphylococcus aureus can cause a range of diseases, such as osteomyelitis, as well as colonize implanted medical devices. In most instances the organism forms biofilms that not only are resistant to the body's defense mechanisms but also display decreased susceptibilities to antibiotics. In the present study, we have examined the effect of increasing silver contents in phosphate-based glasses to prevent the formation of S. aureus biofilms. Silver was found to be an effective bactericidal agent against S. aureus biofilms, and the rate of silver ion release (0.42 to 1.22 µg·mm–2·h–1) from phosphate-based glass was found to account for the variation in its bactericidal effect. Analysis of biofilms by confocal microscopy indicated that they consisted of an upper layer of viable bacteria together with a layer (20 µm) of nonviable cells on the glass surface. Our results showed that regardless of the silver contents in these glasses (10, 15, or 20 mol%) the silver exists in its +1 oxidation state, which is known to be a highly effective bactericidal agent compared to that of silver in other oxidation states (+2 or +3). Analysis of the glasses by 31P nuclear magnetic resonance imaging and high-energy X-ray diffraction showed that it is the structural rearrangement of the phosphate network that is responsible for the variation in silver ion release and the associated bactericidal effectiveness. Thus, an understanding of the glass structure is important in interpreting the in vitro data and also has important clinical implications for the potential use of the phosphate-based glasses in orthopedic applications to deliver silver ions to combat S. aureus biofilm infections
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