178,961 research outputs found

    Biochemical characterization of chromatin from the dinoflagellate Crypthecodinium cohnii, the binucleate dinoflagellate Peridinium balticum and a chromophyte Olisthodiscus luteus

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    Typescript (photocopy).Understanding of the complex genetic control mechanisms of higher organisms may be facilitated by examining primitive organisms that possess much lower degrees of gene complexity, yet have developed advanced modes of gene transcription, replication, and organization. The group of organisms collectively called the algae is of particular interest with respect to chromatin structure due to the tentative classification of many of its members. Classification places a few species close to higher plants while others border on the lower plant-protozoan boundary. Chromatin from a uninucleate dinoflagellate, Crypthecodinium cohnii, a binucleate dinoflagellate, Peridinium balticum and a chromophyte, Olisthodiscus luteus, were examined. Scanning electron microscopy reveals recognizable differences between the dinokaryotic nucleus and the eukaryotic nucleus. The eukaryotic nucleus reveals a very smooth surface when viewed with the scanning microscope and nuclear pores are evident. Scanning electron microscopy revealed a dinokaryotic nucleus whose nuclear envelope was highly convoluted. Higher magnification of the extruded chromatin from the dinokaryotic nucleus reveals fibers ranging from 400 to 500 (ANGSTROM). That these fibers are higher order structures of the unit thread does not seem likely. Similar to higher eukaryotes, staphylococcal nuclease digestion of nuclei isolated from O. luteus reached a limit of 50% digestion. In contrast, digested C. cohnii nuclei only reached a 20% limit of digestion. Digestion of nuclei isolated from P. balticum exhibited characteristics expected from a mixture of dinokaryotic and eukaryotic nuclei. Gel analysis of the products of digestion of the eukaryotic nucleus from Olisthodiscus and Peridinium revealed a basic repeat unit measuring 220 (+OR-) 5 base pairs. Limit digestion gave a core particle of 140 base pairs revealing that these longer repeat sizes are due to longer linker regions. No repeating subunit structure was found upon electrophoresis of digests of C. cohnii nuclei. Examination of the DNA fragments produced by DNase I digestion of nuclei isolated from P. balticum and O. luteus showed the same ladder of 10 base multiples as seen in other chromatin examined. The kinetics of DNase I digestion varied with respect to the three different organisms. C. cohnii nuclei reached a limit of only 20% acid solubility, while about half of the chromatin from Olisthodiscus was sensitive to DNase I. . . . (Author's abstract exceeds stipulated maximum length. Discontinued here with permission of author.) UM

    Simulation of thermal plant optimization and hydraulic aspects of thermal distribution loops for large campuses

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    Following an introduction, the author describes Texas A&M University and its utilities system. After that, the author presents how to construct simulation models for chilled water and heating hot water distribution systems. The simulation model was used in a $2.3 million Ross Street chilled water pipe replacement project at Texas A&M University. A second project conducted at the University of Texas at San Antonio was used as an example to demonstrate how to identify and design an optimal distribution system by using a simulation model. The author found that the minor losses of these closed loop thermal distribution systems are significantly higher than potable water distribution systems. In the second part of the report, the author presents the latest development of software called the Plant Optimization Program, which can simulate cogeneration plant operation, estimate its operation cost and provide optimized operation suggestions. The author also developed detailed simulation models for a gas turbine and heat recovery steam generator and identified significant potential savings. Finally, the author also used a steam turbine as an example to present a multi-regression method on constructing simulation models by using basic statistics and optimization algorithms. This report presents a survey of the author??s working experience at the Energy Systems Laboratory (ESL) at Texas A&M University during the period of January 2002 through March 2004. The purpose of the above work was to allow the author to become familiar with the practice of engineering. The result is that the author knows how to complete a project from start to finish and understands how both technical and nontechnical aspects of a project need to be considered in order to ensure a quality deliverable and bring a project to successful completion. This report concludes that the objectives of the internship were successfully accomplished and that the requirements for the degree of Degree of Engineering have been satisfied

    Intern experience at CH���M Hill, Inc.: an internship report

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    Includes author's vita"Submitted to the College of Engineering of Texas A&M University in partial fulfillment of the requirement for the degree of Doctor of Engineering."Includes bibliographical referencesA review of the author's internship experience with CH���M HILL, Inc. during the period September 1975 through May 1976 is presented. During this nine month internship the author worked as an Engineer II in the Industrial Processes discipline of this large consulting engineering firm... The author's prime responsibility was as one of three lead design engineers on the design of a large wastewater treatment facility for a pulp mill in Hoquiam, Washington owned by ITT Rayonier Inc. The work generally consisted of the design of individual treatment units and associated piping and pumping. The purpose of the project was to provide wastewater treatment capabilities that would satisfy the effluent limitations (standards) imposed upon the mill by the State of Washington Department of Ecology and the U.S. Environmental Protection Agency. The author's assignment also entailed necessary interaction with the project manager and other CH���M HILL design engineers and support staff members, the client's representatives, and representatives of two other consulting engineering firms working on the project. Thus, the internship position at CH���M HILL provided considerable experience coordinating the author's work with the work of other engineers, guiding the design and administrative efforts of a support staff, and interacting regularly with the client and other consulting firms. This broad exposure to a variety of engineering and organizational problems provided a valuable educational experience

    Intern experience at the Texas Transportation Institute: an internship report

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    "Submitted to the College of Engineering of Texas A&M University in partial fulfillment of the requirements for the degree of Doctor of Engineering."Includes vita (leaf 91)Includes bibliographical references (leaf 57)This report discusses the author's engineering experience at the Texas Transportation Institute (TTI), Texas A&M University from March 1974 through July 1977. A report of this experience plus twelve additional hours of academic course work were substituted for an internship according to requirements established by the College of Engineering. Although the author could not retroactively establish objectives of the type associated with a typical internship, the work experience gained could be related to the two general objectives of an intership: 1. To demonstrate an identifiable contribution to the organization in which the intern served, and 2. To enable the intern to become aware of the non-technical aspects of working as an engineer in a non-academic environment. While a member of the Texas Transportation Institute staff, the author worked on a number of projects in the areas of highway traffic engineering research and the preparation of continuing education courses for transportation engineering officials. These assignments were contained within the Urban Transportation Systems Division of TTI. This report delineates the author's contribution to four particular projects and discusses the technical as well as non-technical experience gained from each. Having been exposed to more engineering situations than many of the other Doctor of Engineering students, the author has already formulated some rather specific milestones for the future. The Doctor of Engineering program is discussed in conjunction with its potential for helping the author attain these career goals and objectives. Also, some thoughts are presented regarding the Doctor of Engineering as a viable alternative to the Ph.D. in preparing for a career in engineering higher education

    Effect of rearing and laying house environments on performance of incross egg production type pullets

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    Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to [email protected], referencing the URI of the item.Not availabl

    The long-wavelength view of GG Tau A: rocks in the ring world

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    We present the first detection of GG Tau A at centimetre wavelengths, made with the Arcminute Microkelvin Imager Large Array at a frequency of 16 GHz (λ = 1.8 cm). The source is detected at >6 σrms with an integrated flux density of S16GHz = 249 ± 45 µJy. We use these new centimetre-wave data, in conjunction with additional measurements compiled from the literature, to investigate the long-wavelength tail of the dust emission from this unusual protoplanetary system. We use an MCMC-based method to determine maximum likelihood parameters for a simple parametric spectral model and consider the opacity and mass of the dust contributing to the microwave emission. We derive a dust mass of Md ~ 0.1 Msun, constrain the dimensions of the emitting region and find that the opacity index at λ > 7 mm is less than unity, implying a contribution to the dust population from grains exceeding ~4 cm in size. We suggest that this indicates coagulation within the GG Tau A system has proceeded to the point where dust grains have grown to the size of small rocks with dimensions of a few centimetres. Considering the relatively young age of the GG Tau association in combination with the low derived disc mass, we suggest that this system may provide a useful test case for rapid core accretion planet formation models

    Intern experience at Honeywell, Inc., Large Information Systems Division: an internship report

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    Includes author's vita"Submitted to the College of Engineering of Texas A&M University in partial fulfillment of the requirement for the degree of Doctor of Engineering."Includes bibliographical references (leaves 169-173)This report describes the author's internship assignment at Honeywell Inc. - Large Information Systems Division from January, 1982 through August, 1982. The purpose of the assignment was to fulfill the internship requirements of the Doctor of Engineering Program. During the internship, the author held the position of VLSI design engineer, where he worked on a predefined project. The project's objective was to evaluate how circuit designs based on an advanced CMOS process, can be applied to hardware products at LISD. The internship allowed the author to successfully apply the MOS circuit design training, which ws developed at Texas A&M, to an industrial project. In addition, involvement in different activities and exposure to the various problems not encountered in academia, made this internship an invaluable experience

    \u27Letters Full of Love\u27: Wayne Booth\u27s Theories of the Implied Author and Reader in Brad Marsh\u27s WWII Correspondence

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    This project consists of a qualitative study of the WWII correspondence of Captain Brad Marsh from the collection of letters donated by his daughter on April 26, 2013. I draw from the narrative theory and works of Wayne Booth to examine Marsh’s construction of self as an implied author by examining how he presents his experiences and portrays his emotions. I also examine how Marsh constructs his implied self by paying attention to details such as the information he shares with Lynn and how he presents it. I explore the nature of the implied author and reader in the correspondence and also how the relationship between the implied writer and reader influences the understanding of an unintended audience

    Pastoral Misconduct: The American Black Church Examined

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    In the past, clergy malfeasance was mentioned only in passing by group members or adherents. The subject was invisible and those who studied it were often stigmatized as hostile to religion itself. Today clergy misconduct is acknowledged as a social problem with growing conceptual and theoretical implications. In Pastoral Misconduct, Anson Shupe and Janelle M. EliassonNannini argue that the history and traditions of black pastoral leadership, coupled with the close identity of many black congregants with their pastor, congregation, and racial subculture, creates opportunity structures that facilitate predatory behavior. Familiarity and mutual identity frequently leads victims to drop their normal levels of wariness. Major denominations and minor sects have been studied, but this unique study by Shupe and EliassonNannini pursues nuances of pastoral bad behavior in a new context. This book is not a tabloid treatment of the American black church. In fact, the black church becomes the vehicle for a major new sociological development: a theory of clergy misconduct in any minority religion.https://digitalcommons.tacoma.uw.edu/socialwork_books/1004/thumbnail.jp

    Intern Experience at MEL, Inc.: An Internship Report

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    Includes author's vita"Submitted to the College of Engineering of Texas A&M University in partial fulfillment of the requirement for the degree of Doctor of Engineering."Includes bibliographical references (leaves 82-85)...The author spent a one-year internship at MEL, Inc., a civil and mechanical engineering consulting firm located in Baton Rouge, Louisiana. By special arrangement with the president of the firm, the author was able to participate in roles at various levels within the firm. These activities included engineering design, supervision and management, project cost control, and interface with top level management. During this period, the author has been engaged in the following assignments: 1. Serving as senior project engineer on the Louisiana Training Institution air conditioning and heating project, 2. Serving at the review and advisory level for three projects, 3. Studying and implementing computer aided design and drafting system for MEL, Inc. 4. Developing a projet cost control system that is interactive with the firm's payroll program. 5. Upgrading the firm's computer capacity. 6. Performing special assignments from the President, Executive Vice-President, Manager of Operation and Manager of Business and Fiscal Affairs at MEL, Inc. These activities involved the application of scientific principles to the design, installation, and improvement of integrated systems of people, materials, and equipment to provide the most effective operating and work procedures. As a result of this involvement in a wide range of assignments, the author now has a broadly based experience in a consulting engineering firm as both engineer and manager. The author also found that well-developed, practical courses in communication, business, engineering, law, and management from the Doctor of Engineering program at Texas A&M University contributed greatly to this successful internship with MEL, Incorporated
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