149,901 research outputs found
The Shu complex is a conserved regulator of Rad51 filament formation
No full textThe budding yeast Shu complex, a heterotetramer of Shu1, Shu2, Csm2, and Psy3, is important for homologous recombination (HR)-mediated chromosome damage repair and was first characterized a decade ago as promoting Rad51-dependent HR in response to replicative stress, but its mechanistic function and conservation in eukaryotes has remained unknown. Here we provide evidence that the Shu complex is evolutionarily conserved throughout eukaryotes, where it is comprised of a clear Shu2 orthologue physically associating with Rad51 paralogues. The Shu complex itself physically interacts with the rest of the HR machinery during DNA damage repair. Finally, we uncover that the mechanistic function of the Shu complex as a stimulatory co-factor of Rad51 filament formation in vitro, likely explaining the in vivo function of the eukaryotic Shu complex in suppressing error-prone repair. Moving forward, our findings provide a framework for studying the function of the human Shu complex, which will have broad importance in our understanding of DNA damage repair
The role of the yeast Shu complex in the error-free bypass of abasic sites and 3-Methylcytosines
No full textAccurate DNA replication is critical to prevent genomic instability, which is a hallmark of cancer. Homologous recombination (HR) is responsible for error-free DNA damage bypass during replication. The yeast Shu complex is a recombination mediator primarily specialized in preventing replication-associated mutagenesis from endogenous DNA lesions as well as those from the alkylating agent methyl methanesulfonate (MMS). However, it remains unclear which are the specific DNA lesions that are tolerated by a Shu complex-mediated error-free pathway. To approach this problem, we performed a genome-wide sequencing of Shu complex disrupted cells chronically exposed to MMS. The analysis of the mutation pattern suggested abasic sites and 3-Methylcytosines as major contributors of mutagenesis in Shu complex deficient cells exposed to MMS. We, therefore, thought to validate these observations. In this work, we found that the Shu complex is enriched at the chromatin in cells that accumulate abasic sites and that it is important for the error-free bypass of APOBE3B-induced abasic sites. Moreover, we also showed that ectopic expression of the 3-Methylcytosine repair enzyme, ALKBH2, specifically rescues MMS-induced phenotypes seen in Shu complex mutant cells, such as growth defects and increased mutagenesis. Overall, our results demonstrate that yeast cells rely on a Shu complex-mediated error-free pathway to prevent mutagenesis from abasic sites and 3-Methylcytosines
Stronger Together Newsletter, April 2023
No full textContents: Letter from the Chief Diversity and Inclusion Officer, Maurice D. Nelson -- Introducing MACC Pioneers (Mentors Advocating for Cultural Connection) -- Sexual and Gender Equity (SAGE) Center transitioning to OIE -- Inclusive excellence education at SHU -- Multicultural events -- SHU student naqmed AAIDN/CIEE Intern Scholar -- Inclusive Excellence Faculty and Staff Fellowship program -- Spotlights and Events
Stronger Together Newsletter, September 2023
No full textWelcome to the 2023-2024 academic year / Maurice D. Nelson -- New SHU chosen name policy -- Multicultural Affairs kickbacks and cookouts -- New MACC Pioneers program -- Gender Sexuality Alliance(GSA) held its Second Annual Queerientation -- New Bias Education Support Team (BEST) -- Juneteenth -- Pride Month -- SHU Chief Diversity and Inclusion Officer Maurice Nelson opinion on the Supreme Court ruling on race-based admissions -- Faculty grants -- Dr. Sue Goncalves receives certification from the AACN-sponsored Diversity Leadership Institute -- Anne Burmeister presents at a second convening of DEI leadership
Spectrum, Volume 35, Issue 1
No full textHighlights include: Interview with New York Times #i Best Selling Author of Wild, Cheryl Strayed --The SHU women’s vocal quartet, also known as UnFOURgettable, will no longer be a group on campus due to the lack of an accompanist --Online Startup Connecting College Students with Odd Jobs --Navigating freshman year: tips and tricks from seasoned SHU students Melanie Vollono and Giovanna Gatto --Spectrum editorial board 2015-2017 (portraits) --Get to know the Greek societies on campus --College student Kaitlyn Kreitzman keeps her life in balance with yoga --Report on fifth annual Presidential Gala --Blogger of the week: President Petillo --Inside look at the upcoming Theatre Arts program Time Stands Still --Spectrum Exclusive: Interview with former ESPN President George Bodenheimer --Head Coach Mark Nofri brought in three transfer students and two coaches to join the team. Fifth year seniors Chris Hutton, Phil Paulhill, and junior Khaamal Whitaker, are the playing additions, while Mike Livingston and Patrick Saporito are the new assistant coaches --Women\u27s rugby begins D-I campaign
DIETARY CONDITIONS AND DIFFERENTIAL ACCESS TO FOOD RESOURCES AMONG THE VARIOUS CLASSES DURING THE HAN PERIOD
No full textIn this thesis, I study how food resources and dietary conditions were determined by social and economic status during the Han period in China, B.C. 206~A.D.220. Even though earlier scholars have published research concerning the Chinese food culture of this period, these studies were limited in that they only illustrated the dietary culture of the upper class or the available food resources in one geographic area. Also, without any persuasive data, it has been assumed by these earlier scholars that there were big differences in food resources and food consumption between the upper and lower classes. In this thesis, for comparison among the classes, I divide the social and economic classes into five stratified groups: nobles, officials, peasants, soldiers and convicts. After a brief introduction of the nature of each social class, I examine the food resources and nutritional condition of each group using information such as the wealth and income of each group, the market price of food resources, the agricultural products of peasants, and the amount of food distribution to soldiers and convicts. I found these data from archaeological remains, received historical records and pictorial data, and excavated texts. This research shows a broader view of Chinese dietary condition focusing not only on the variety of food resources of nobles, but also on the different food accessibilities among the officials, and the food deficiencies of peasants. It also deals with the situations of food supply for soldiers and convicts in an effort to reveal the true dietary consumption and nutritional conditions for all Chinese. This research proves that the various classes during the Han period in China had different food resources and dietary conditions
Man Shu, piano, February 27, 2016
No full textThis is the concert program of the Man Shu, piano performance on Saturday, February 27, 2016 at 4:30 p.m., at the Concert Hall, 855 Commonwealth Avenue. Works performed were Piano Sonata No. 18 in G major, D. 894 by Franz Schubert, Le tombeau de Couperin by Maurice Ravel, Années de pèlerinage, Book I, S.160 by Franz Liszt, and 3 Preludes for Piano by George Gershwin. Digitization for Boston University Concert Programs was supported by the Boston University Humanities Library Endowed Fund
MeSH term explosion and author rank improve expert recommendations
No full textInformation overload is an often-cited phenomenon that reduces the productivity, efficiency and efficacy of scientists. One challenge for scientists is to find appropriate collaborators in their research. The literature describes various solutions to the problem of expertise location, but most current approaches do not appear to be very suitable for expert recommendations in biomedical research. In this study, we present the development and initial evaluation of a vector space model-based algorithm to calculate researcher similarity using four inputs: 1) MeSH terms of publications; 2) MeSH terms and author rank; 3) exploded MeSH terms; and 4) exploded MeSH terms and author rank. We developed and evaluated the algorithm using a data set of 17,525 authors and their 22,542 papers. On average, our algorithms correctly predicted 2.5 of the top 5/10 coauthors of individual scientists. Exploded MeSH and author rank outperformed all other algorithms in accuracy, followed closely by MeSH and author rank. Our results show that the accuracy of MeSH term-based matching can be enhanced with other metadata such as author rank
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