8,494 research outputs found
Proline-rich tyrosine kinase 2 mediates gonadotropin-releasing hormone signaling to a specific extracellularly regulated kinase-sensitive transcriptional locus in the luteinizing hormone beta-subunit gene
Get PDFG protein-coupled receptor regulation of gene transcription primarily occurs through the phosphorylation of transcription factors by MAPKs. This requires transduction of an activating signal via scaffold proteins that can ultimately determine the outcome by binding signaling kinases and adapter proteins with effects on the target transcription factor and locus of activation. By investigating these mechanisms, we have elucidated how pituitary gonadotrope cells decode an input GnRH signal into coherent transcriptional output from the LH beta-subunit gene promoter. We show that GnRH activates c-Src and multiple members of the MAPK family, c-Jun NH2-terminal kinase 1/2, p38MAPK, and ERK1/2. Using dominant-negative point mutations and chemical inhibitors, we identified that calcium-dependent proline-rich tyrosine kinase 2 specifically acts as a scaffold for a focal adhesion/cytoskeleton-dependent complex comprised of c-Src, Grb2, and mSos that translocates an ERK-activating signal to the nucleus. The locus of action of ERK was specifically mapped to early growth response-1 (Egr-1) DNA binding sites within the LH beta-subunit gene proximal promoter, which was also activated by p38MAPK, but not c-Jun NH2-terminal kinase 1/2. Egr-1 was confirmed as the transcription factor target of ERK and p38MAPK by blockade of protein expression, transcriptional activity, and DNA binding. We have identified a novel GnRH-activated proline-rich tyrosine kinase 2-dependent ERK-mediated signal transduction pathway that specifically regulates Egr-1 activation of the LH beta-subunit proximal gene promoter, and thus provide insight into the molecular mechanisms required for differential regulation of gonadotropin gene expression
Resilient cooling strategies: a preliminary study of an all-air system application
No full textResilient cooling is the ability of a building-plant system to withstand and recover after a disruptive event. In particular, heatwaves and power outages have been considered in this study as perturbations of a building-plant system. The present work investigated the operation of an all-air HVAC system combined with two air to water heat pumps in Copenhagen, Denmark. TRNSYS simulations considered both current and future outdoor climatic conditions, both in mid and long term, to evaluate building’s energy use. The results showed that during heatwaves operative temperatures higher than 28 °C were registered, but only for 5 to 20% of the occupied time in the worst scenario. The variation was due to the cooling load, which increased as the heatwave increased in length and intensity. Nevertheless, the energy use did not considerably increase with values between 33 to 39 kWh/(m2 y) but the system resulted to be non-resilient in most of the cases that combine heatwaves with power outages
author
No full textauthorIn other places [Old Joe, the trapper] pointed out otter (or, as he pronounced it, "author") slidesPRINTED ITEM G.M.Story April 1959Not UsedNot UsedWithdrawn[see 'otter']Checked by Jordyn Hughes on Thu 09 Jun 201
Dense ceramic coatings deposited by aerosol deposition for multilayered architecture towards thermal/environmental barrier coatings
Get PDFThe demands for thermal/environmental barrier coatings (T/EBCs) have been increased as the operating temperature of gas turbines increased in harsh environment [1]. Multilayered and multifunctional coatings are required for advanced T/EBCs [2], varied from porous insulative layer to dense environmental barrier layer. Aerosol deposition (AD) method is a unique deposition method that enables the deposition of dense ceramic coatings with high adhesion strength without melting of injected powder based on room temperature impact consolidation (RTIC) phenomena [3-5]. Thus, it will be interesting to apply this process for T/EBC applications. However, in order to apply the AD method to these applications, the deposition rate and the ability of three-dimensional coverage should be improved. Mori et al. preliminary reported that the introduction of plasma assistance drastically improved the deposition rate for lead zirconate titanate [6]. Thus, it would be worth to try to enhance aerosol deposition by introduction of plasma assistance [7]. The use of mesoplasma flow, which is transitional state from thermal plasma to low-pressure plasma, is the key to the deposition [8]. Fine powder of 8-wt% yttria-stabilized zirconia was sprayed by an rf-inductively coupled plasma at a reduced pressure. The effect of plasma assistance was confirmed at the power input of several kilowatts, which was much smaller compared to conventional plasma spray. Coatings with uniform thickness of 5-20 µm was obtained. The Vickers hardness of the coating reached to 1200 HV. This coating will be useful for the architecture of multilayered advanced T/EBCs.
References
[1] J. M. Drexler et al., Adv. Mater., 23 (2011) 2419-2424.
[2] V. Viswanathan et al., J. Am. Ceram. Soc., 97 (2014) 2770-2778.
[3] J. Akedo and M. Levedev, Jpn. J. Appl. Phys. Part 1, 38 (1999) 5397-5401.
[4] J. Akedo, J. Am. Ceram. Soc. 89 (2006) 1834-1839.
[5] J. Akedo, J. Therm. Spray Technol. 17 (2008) 181-198.
[6] M. Mori et al., Proc. IEEE Int. Symp. Appl. Ferroelectric. 1-2 (2007) 454-456.
[7] A. Vardelle et al., J. Therm. Spray Technol. 25 (2016) 1376-1440 (J. Akedo and K. Shinoda, Section 2.2, 1379-1383).
[8] T. Yoshida, Pure Appl. Chem. 78 (2006) 1093-1107
Jia ru ju he wu dui jun yun tuan liu ji jun yun tuan liu dui liu de ying xiang
No full textWong, Chai Kwok = 加入聚合物對均勻湍流及均勻湍流對流的影響 / 黃濟國.Thesis M.Phil. Chinese University of Hong Kong 2013.Includes bibliographical references (leaves 89-91).Abstracts also in Chinese.Title from PDF title page (viewed on 01, November, 2016).Wong, Chai Kwok = Jia ru ju he wu dui jun yun tuan liu ji jun yun tuan liu dui liu de ying xiang / Huang Jiguo
Author Correction: Manipulating anion intercalation enables a high-voltage aqueous dual ion battery
No full textThe original version of this Article did not acknowledge Prof. Jun Fan as a corresponding author. This has now been corrected in both the PDF and HTML versions of the Article.link_to_subscribed_fulltex
Author Correction: A proteomic landscape of diffuse-type gastric cancer
Get PDFThe original version of this Article contained an error in the email address of the corresponding author Jun Qin. The correct email is [email protected]. The error has been corrected in the HTML and PDF versions of the Article.</jats:p
Corrigendum: The taeniaticornis-group of genus Apanteles Foerster (Hymenoptera, Braconidae, Microgastrinae) from China with one new species. Journal of Hymenoptera Research 96: 21–31. doi: 10.3897/jhr.96.99649
Get PDFIn a paper about a new species of Apanteles (Microgastrinae)(Liu & Chen, 2023), we regret the omission of one author Jun-hua Chen in the second place of the author list who did great job in construction of the ZJUH collection for this study and the mistake in institution order and corresponding author. We provide the correct information below.Zhen Liu1, 2, Jun-hua He1, Xue-xin Chen11 Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. 2 Zoology Key Laboratory of Hunan Higher Education, College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China
Yong yu you hua zhi bei chao leng yuan zi hun he wu de guang xue ou ji jing
No full textChen, Jun = 用於優化製備超冷原子混合物的光學偶極阱 / 陳軍.Thesis M.Phil. Chinese University of Hong Kong 2014.Includes bibliographical references (leaves 109-117).Abstracts also in Chinese.Title from PDF title page (viewed on 29, November, 2016).Chen, Jun = Yong yu you hua zhi bei chao leng yuan zi hun he wu de guang xue ou ji jing / Chen Jun
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