3,434 research outputs found

    Suppression of the Pth(Ts) phenotype mediated by the overproduction of tRNA maintains moderate levels of the Pth(Ts) protein

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    <p><b>Copyright information:</b></p><p>Taken from "Excess of charged tRNA maintains low levels of peptidyl-tRNA hydrolase in (Ts) mutants at a non-permissive temperature"</p><p>Nucleic Acids Research 2006;34(5):1564-1570.</p><p>Published online 15 Mar 2006</p><p>PMCID:PMC1408313.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () Depicts the cellular growth of the (Ts) mutant strain AA7852 separately transformed with pVH124 (ΔU, ΔV), pVH125 (U, ΔV) or pVH119 (U, V) incubated at different temperatures. Isolated colonies of the independent transformants were streaked onto LB-Ap plates and incubated overnight at the indicated temperatures. () Presents the immunodetection of Pth(Ts) in the (Ts) mutant strain AA7852 separately transformed with pVH124, pVH125, pVH119, ptRNACCA (X, R, T, M) or pTH2 (W) and grown at 32°C prior to transfer at time = 0 min at 41 or 43°C. The concentration of Pth(Ts) protein was estimated by immunoblot analysis. The left lane shows purified wild-type Pth protein, which migrates slightly faster in SDS–PAGE than the Pth(Ts) variant (arrowed) ()

    Emergence of oblique TS mode due to longitudinal wall oscillation in 2D channel flow

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    Stabilizing effects of wall oscillation on two dimensional (2D) Tollmein-Schlichting (TS) waves and oblique TS waves developing in 2D channel flow are numerically investigated using the Floquet method. It was shown from the previous study[1] that the wall oscillation mostly has stabilizing effect and then TS mode is occasionally more stable than the oblique mode. In the present study the characteristics of the stability are investigated in detail. Then it is cleared that the exchange of the most unstable mode form the 2D TS to the oblique TS can occur at a certain value of Reynolds number
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