2,588 research outputs found
Data_Sheet_1_Microbial degradation of aristolochic acid I by endophytic fungus A.h-Fs-1 of Asarum heterotropoides.zip
No full textAsari Radix et Rhizoma is commonly used in classic prescriptions of herbal medicine in several Asian countries for resuscitation, pain relief, and sore treatment, and Asarum heterotropoides (A. heterotropoides) is an important source material of Asari Radix et Rhizoma. However, the plants of the Asari Radix et Rhizoma and some plants in Asarum spp. contain aristolochic acid I (AAI), which is considered as a carcinogen. The objective of the current study is to detoxify Asarum spp. through microbial degradation of AAI in order to ensure drug safety. Based on the observation of the close correlation between endophytic fungi of A. heterotropoides and AAI, we identified an AAI-degrading fungus and screened for candidate genes involved in AAI degradation. Full-length O-demethylase genes (ODMs) were cloned including A.h-ODM-5, Fs-ODM-4, and Fs-ODM-1, and their ability to degrade AAI was tested in vitro. The results showed that the AAI-degrading fungus was identified as Neocosmospora solani (A.h-Fs-1, endophytic fungi of A. heterotropoides), and verified the capability of specific O-demethylation to modify the structure of AAI. We further identified the functional ODMs in A.h-Fs-1 capable of degrading AAI and uncovered the AAI degradation mechanism of A.h-Fs-1. The microbial degradation of AAI demonstrated in the present study offers a new method to detoxify plant materials used for herbal medicine, and would enhance the regulation of toxic ingredients content in herbal medicine source materials.</p
The construction of Karen Karnak: The multi-author-function
No full textThis thesis is situated within the comparatively recent developments of Web 2.0 and the emergence of interactive WikiMedia, and explores the mode of authorship within a Read/Write culture compared to that of a Read/Only tradition. The hypothesis of this study is that the role of the audience has become merged with the author, and as such, represents new functions and attributes, distinct from a more conventional concept of authorship, in which the roles of audience and author are more separate. Read/Write and participatory culture, as defined by this study, is focused on collaboration, and includes the influences of D.I.Y. culture, Open-Source practices and the production of text by multiple authors. Multi-authorship presents a re-thinking of several concepts which support the notion of the individual author, since the focus of multi-authorship is not on attribution and ownership of a finished text, but on the continued malleability of a text. Modes of multi-authorship, demonstrated in the use of the pseudonyms Alan Smithee and Karen Eliot, represent declarative authors whose names signify multiple origins, whilst concurrently indicating a distinct body of work. The function of these names form an important context to this study, since primary research involves the construction of an experimental mode of multi-authorship utilising WikiMedia technology and the interaction of thirty nine participants, who are invited to create a body of work under the collective pseudonym Karen Karnak. The data generated by this experiment is analysed using aspects of Michel Foucault's author-function to identify and determine power structures inherent in the WikiMedia context. The interplay of power structures, including concepts such as identity, ownership and the body of work, affect the resulting mode of authorship and contribute to the construction of Karen Karnak, suggesting further areas of research into the emerging multi-author
FSD-FS
No full textFSD-FS is a publicly-available database of human labelled sound events for few-shot learning. It spans across 143 classes obtained from the AudioSet Ontology and contains 43805 raw audio files collected from the FSD50K. FSD-FS is curated at the Centre for Digital Music, Queen Mary University of London.
Citation
If you use the FSD-FS dataset, please cite our paper and FSD50K.
@article{liang2022learning,
title={Learning from Taxonomy: Multi-label Few-Shot Classification for Everyday Sound Recognition},
author={Liang, Jinhua and Phan, Huy and Benetos, Emmanouil},
journal={arXiv preprint arXiv:2212.08952},
year={2022}
}
@ARTICLE{9645159, author={Fonseca, Eduardo and Favory, Xavier and Pons, Jordi and Font, Frederic and Serra, Xavier}, journal={IEEE/ACM Transactions on Audio, Speech, and Language Processing}, title={FSD50K: An Open Dataset of Human-Labeled Sound Events}, year={2022}, volume={30}, number={}, pages={829-852}, doi={10.1109/TASLP.2021.3133208}}
About FSD-FS
FSD-FS is an open database for multi-label few-shot audio classification containing 143 classes drawn from the FSD50K. It also inherits the AudioSet Ontology. FSD-FS follows the ratio 7:2:1 to split classes into base, validation, and evaluation sets, so there are 98 classes in the base set, 30 classes in the validation set, and 15 classes in the evaluation set (More details can be found in our paper).
LICENSE
FSD-FS are released in Creative Commons (CC) licenses. Same as FSD50K, each clip has its own license as defined by the clip uploader in Freesound, some of them requiring attribution to their original authors and some forbidding further commercial reuse. For more details, ones can refer to the link.
FILES
FSD-FS are organised in the structure:
root
|
└─── dev_base
|
└─── dev_val
|
└─── eval
REFERENCES AND LINKS
[1] Gemmeke, Jort F., et al. "Audio set: An ontology and human-labeled dataset for audio events." 2017 IEEE international conference on acoustics, speech and signal processing (ICASSP). IEEE, 2017. [paper] [link]
[2] Fonseca, Eduardo, et al. "Fsd50k: an open dataset of human-labeled sound events." IEEE/ACM Transactions on Audio, Speech, and Language Processing 30 (2021): 829-852. [paper] [code
Yb-doped femtosecond lasers and their frequency doubling
No full textUltralow threshold, compact and highly efficient femtosecond lasers based on Yb³⁺-doped
potassium yttrium tungstate (Yb:KYW) and Yb³⁺-doped vanadium yttrium oxide
(Yb:YVO 4 ) have been demonstrated within this PhD-research project. For a continuous
wave unmode-locked Yb:KYW laser a threshold as low as 101 mW was obtained with a
slope efficiency of 74%. By employing a single prism for dispersion control, the laser was
tunable between 1012 nm to 1069 nm. When operated in the mode-locked regime, this
laser produced transform-limited pulses having durations of 210 fs at a central wavelength
of 1044 nm. Stable mode locking was observed for an optimised incident pulse fluence on
the SESAM between 140 μJ/cm²
to 160 μJ/cm²
which was 2-3 times higher than the
designed energy pulse fluence of the SESAM (70 μJ/cm²).
The employment of several combinations of chirped mirror designs for control of
intracavity group velocity dispersion led to excellent results. The threshold for mode
locking was satisfied for a pump power of 255 mW where the slope efficiency was
measured to be 62%. This is the most efficient SESAM-assisted femtosecond laser yet
reported and the highest optical-to-optical efficiency of 37% is exceptional. Transform-limited pulses with durations as short as 90 fs were produced in a spectral region centred
on 1052 nm. The success of this research thus represents a good foundation on which to
design and build more compact configurations that will incorporate just one chirped mirror
for dispersion compensation.
A relatively high nonlinear refractive index, n₂ , of 15 x 10⁻¹⁶ cm²/W was measured
in Yb:YVO₄ and this affords particular potential for this candidate material in Kerr-lens
mode locking. In fact, for operation in the femtosecond domain, the threshold power was
190 mW with a slope efficiency of 26% and near-transform-limited pulses as short as 61fs were generated at a centre wavelength of 1050 nm. The main objectives in developing
this type of laser relate to a demonstration of high peak power operation in thin disc laser
configurations.
The deployment of a diode-pumped Yb:KYW femtosecond laser as a pump source
for frequency doubling in a periodically-poled LiTaO₃ crystal was realised. The maximum
realized output power of 150 mW corresponded to an impressive second harmonic
conversion efficiency of 43%. 225-fs duration green pulses (centred at 525 nm) were
generated under the condition of strong focusing in the nonlinear crystal
Experimental Investigation Contrast to Coal Dust and FS Powder Application
No full textWe preliminary know FS powder and coal dust play in mix sand and analysis comparison influence of each powder through a series of mixture sand performance testing . We put forward on same addition, FS powder substitute coal dust is profitable to reduce defect of M593 Shell structure adhering sand.</jats:p
Tissue architecture of the anterior pituitary showing the epithelial cell cords with hormonal cells and folliculo-stellate (FS) cells, the capillaries (C) with fenestrated endothelial cells (EC) and connective tissue (CT)
No full text<p><b>Copyright information:</b></p><p>Taken from "Paracrinicity: The Story of 30 Years of Cellular Pituitary Crosstalk"</p><p></p><p>Journal of Neuroendocrinology 2008;20(1):1-70.</p><p>Published online Jan 2008</p><p>PMCID:PMC2229370.</p><p>© 2008 The Author. Journal Compilation © 2008 Blackwell Publishing Ltd</p> The cell cords are a cluster of endocrine cells surrounding an aggregate of FS cells that make a follicle (F). FS cells also make a meshwork between the hormonal cells, making junctions among each other (thick lines) and extending foot processes (f) ending on the basal membrane (BM) in the periphery of the cord. The cords are surrounded by BM, which may have extensions between some cells. A second BM surrounds the capillary vessels and between these two some connective tissue resides. Small and larger lacunae are present between hormonal cells. Paracrine substances may circulate from cell-to-cell but also could be released in these lacunae and reach more remote places. FS cells make gap junctions mostly among each other, but occasionally also with some hormonal cells. Hormonal cells can make interdigitations with FS cells (small arrows) to favour cell-to-cell communication. Adapted from Vila-Porcile ()
Adaptive pulse compression for transform-limited 15-fs high-energy pulse generation
No full textWe demonstrate the use of a deformable-mirror pulse shaper, combined with an evolutionary optimization algorithm, to correct high-order residual phase aberrations in a 1-mJ, 1-kHz, 15-fs laser amplifier. Frequency resolved optical gating measurements reveal that the output pulse duration of 15.2 fs is within our measurement error of the theoretical transform limit. This technique significantly reduces the pulse duration and the temporal prepulse energy of the pulse while increasing the peak intensity by 26%. It is demonstrated, for what is believed to be the first time, that the problem of pedestals in laser amplifiers can be addressed by spectral-domain correction
Self-compression of 4.9 µm pulses to sub-40 fs with 2 mJ energy in Zinc Sulfide
No full textNonlinear self-compression of few-cycle multi-mJ pulses at 4.9 µm in ZnS is presented. 80 fs input pulses are compressed to 37 fs with 2.1 mJ energy at a 1 kHz repetition rate. © 2024 The Author(s
Minor effect of physical size sorting on iron solubility of transported mineral dust
No full textObservations show that the fractional solubility of Fe (FS-Fe, ratio of dissolved to total Fe) in dust aerosol increases dramatically from ~0.1% in regions of high dust mass concentration to 80% in remote regions where concentrations are low. Here, we combined laboratory geochemical measurements with global aerosol model simulations to test the hypothesis that the increase in FS-Fe is due to physical size sorting during transport. We determined the FS-Fe and fractional solubility of Al (FS-Al) in size-fractionated dust generated from two representative soil samples collected from known Saharan dust source regions using a customized dust re-suspension and collection system. The results show that the FS-Fe is size-dependent and ranges from 0.1-0.3 % in the coarse size fractions (>1 µm) to ~0.2-0.8 % in the fine size fractions
Microbial degradation of aristolochic acid I by endophytic fungus A.h-Fs-1 of Asarum heterotropoides
No full textAsari Radix et Rhizoma is commonly used in classic prescriptions of herbal medicine in several Asian countries for resuscitation, pain relief, and sore treatment, and Asarum heterotropoides (A. heterotropoides) is an important source material of Asari Radix et Rhizoma. However, the plants of the Asari Radix et Rhizoma and some plants in Asarum spp. contain aristolochic acid I (AAI), which is considered as a carcinogen. The objective of the current study is to detoxify Asarum spp. through microbial degradation of AAI in order to ensure drug safety. Based on the observation of the close correlation between endophytic fungi of A. heterotropoides and AAI, we identified an AAI-degrading fungus and screened for candidate genes involved in AAI degradation. Full-length O-demethylase genes (ODMs) were cloned including A.h-ODM-5, Fs-ODM-4, and Fs-ODM-1, and their ability to degrade AAI was tested in vitro. The results showed that the AAI-degrading fungus was identified as Neocosmospora solani (A.h-Fs-1, endophytic fungi of A. heterotropoides), and verified the capability of specific O-demethylation to modify the structure of AAI. We further identified the functional ODMs in A.h-Fs-1 capable of degrading AAI and uncovered the AAI degradation mechanism of A.h-Fs-1. The microbial degradation of AAI demonstrated in the present study offers a new method to detoxify plant materials used for herbal medicine, and would enhance the regulation of toxic ingredients content in herbal medicine source materials
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