40 research outputs found
Microbial expression profiles in the rhizosphere of two maize lines differing in N use efficiency
No full textStudy of the microbial expression profile in the rhizosphere of two contrasting maize lines, differing in the Nitrogen Use efficiency (NUE). The Lo5 and T250 inbred maize characterized by high and low NUE, respectively, were grown inrhizoboxes allowing precise sampling of rhizosphere and bulk soils. We conducted metatranscriptomic of rhizosphere and bulk soil by m-RNA sequencing. High activity of bacteria was observed com-pared to archaea and fungi in both rhizosphere and bulk soils of both maize lines. Proteobacteria and Actinobacteria were involved in all processes, while significant shifts occurred in the expression of Bacteroidetes, Chloroflexi, Firmicutes, Acidobacteria, Cyanobacteria , archaea and fungi, indicating their possible role in specific processes occurring in rhizo-sphere of two maize lines. Maize plants with different NUE induced changes in microbial processes, especially in N cycling, with high NUE maize favouring ammo-nification and nitrification processes and low NUE maize inducing expression of genes encoding for denitrifying process, likely favoured by longer N residence time in the rhizosphere. Theseresults can improve our understanding on plant-microbe interaction in the rhizosphere of crop plantswith potential applications for improving the manage-ment practices of the agro-ecosystems
Enzyme activity and microbial community structure in the rhizosphere of two maize lines differing in N use efficiency
No full textAims: Study of the changes in soil microbial biomass, enzyme activity and the microbial community structure in the rhizosphere of two contrasting maize lines differing in the nitrogen use efficiency (NUE).
Methods: The Lo5 and T250 inbred maize characterized by high and low NUE, respectively, were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil and solution. We also determined microbial biomass, enzyme activities involved in the C, N, P and S cycles, and the microbial community structure using a phylogenetic group specific PCR-DGGE approach in the rhizosphere and bulk soil of both Lo5 and T250 maize lines.
Results: High NUE Lo5 maize induced faster inorganic N depletion in the rhizosphere and larger changes in microbial biomass and enzyme activities than the low NUE T250 maize line. The two maize lines induced differences in the studied microbial groups in the rhizosphere, with the larger modifications induced by the high NUE Lo5 maize line.
Conclusions: The Lo5 maize line with higher NUE induced larger changes in soil chemical properties and in the enzyme activity, soil microbial biomass and community structure than the low NUE T250 maize line, probably due to differences in the root exudates of the two maize lines
Microbial β-glucosidase genesin soil: Molecular diversity,gene expression and enzymatic activity
No full textVariations of soil organic carbon fractions in response to conservative vegetation successions on the Loess Plateau of China
No full textDiversified crop rotation improves continuous monocropping eggplant production by altering the soil microbial community and biochemical properties
No full textEcotone Dynamics and Stability from Soil Scientific Point of View
No full textTransitional areas between two or more different biomes—ecotones—are clearly visible due to the sudden changes in vegetation structures and patterns. However, much is still unknown about the crucial soil factors that control such vegetational changes across ecotones and how different soil properties vary across ecotones. In this study, we try to understand the spatial variation in soil properties across a clearly defined ecotone from a forest stand to meadow field at the Training Forest Enterprise (T.F.E), Masaryk Forest Křtiny, Czechia. Thirteen sampling sites were selected: six in the forest region, six in the meadow and one in the ecotone zone between forest and meadow. Soil samples were taken at 5 cm below the soil surface once every month from April to November. All the collected soil samples were examined for minimal air capacity, actual and potential soil reaction and maximum capillary water. The results showed a pattern of soil acidity decreasing from the forest stand towards the meadow field but that increased sharply at the ecotone zone. The water holding capacity showed a decreasing trend approaching the ecotone zone from the meadow region and markedly decreased from the meadow site closest to the ecotone zone. The minimum air capacity showed an increasing trend from the forest region but suddenly declined at the ecotone region
Microbial expression profiles in the rhizosphere of two maize lines differing in N use efficiency
No full text
Maize lines with different nitrogen use efficiency select bacterial communities with different β-glucosidase-encoding genes and glucosidase activity in the rhizosphere
No full textWe studied the molecular diversity of β-glucosidase-encoding genes, microbial biomass, cellulase, N-acetyl-glucosaminidase, β-glucosidase, and β-galactosidase activities in the rhizosphere and bulk soil of two maize lines differing in nitrogen use efficiency (NUE). The maize lines had significant differences in diversity of β-glucosidase-encoding genes in their rhizosphere, and Actinobacteria and Proteobacteria were the dominating phyla in all samples, but representatives of Bacteroidetes, Chloroflexi, Deinococcus-Thermus, Firmicutes, and Cyanobacteria were also detected. Among the Proteobacteria, β-glucosidase genes from α-, β-, and γ-Proteobacteria were dominant in the rhizosphere of the high NUE maize line, whereas δ-Proteobacteria β-glucosidase genes were dominant in the rhizosphere of the low NUE maize line. The high NUE maize line also showed higher glucosidase activities in the rhizosphere than the low NUE maize line. We concluded that plants with high NUE select bacterial communities in the rhizosphere differing in the diversity of β-glucosidase-encoding genes which likely result in higher C-hydrolyzing enzyme activities. These effects on the diversity of β-glucosidase-encoding genes may influence the C dynamics in the agro-ecosystems
