1,721,166 research outputs found
Measurement of mitochondrial membrane potential and proton leak
No full textThe major component of mitochondrial electrochemical potential gradient of protons is the mitochondrial membrane potential (δψ), and hence it is a suitable parameter for assessment of mitochondrial function. Dissipation of the mitochondrial membrane potential causes uncoupling of the electron transport through the respiratory chain and the phosphorylation reaction for ATP synthesis (proton leak). Proton leak functions as a regulator of mitochondrial reactive oxygen species (ROS) production and its modulation by uncoupling proteins, which may be involved in pathophysiology. In this report, we describe the assays for mitochondrial membrane potential and proton leak, which require a TPP+ electrode and a Clark electrode. The determination of mitochondrial peroxide production with homovanillic acid is also included. © 2010 Humana Press, a part of Springer Science + Business Media, LLC
Impatto della terapia con Peg-interferone o entecavir sulla fibrosi epatica mediante elastomeri: osservazione dopo 4 anni
No full textClinical, diagnostic features and complications of hand, foot, and mouth disease caused by coxsackievirus A6 in children and adults
No full textAutoimmune liver disease and multiple sclerosis: state of the art and future perspectives
No full textMiRNA 106a-5p in cerebrospinal fluid as signature of early relapsing remitting multiple sclerosis: a cross sectional study
No full textHypoxic reperfusion limits oxidative stress and improves myocardial post ischemic functional recovery
No full textDrug hypersensitivity cutaneous diseases in the elderly
No full textThe worldwide increase of life expectancy, changes in immunological capacity, comorbidities and polytherapy are responsible for the increasing prevalence of geriatric drug related allergic skin diseases. In the elderly other factors contribute to the onset of these phenomena, such as changes in the structure of the skin and mucous tissues. The integrity of the epithelial barrier in old people is compromised by the loss of its constituents, that predisposes to alterations of the hydrolipidic film with dryness, xerosis and pruritus. Also at the skin level are frequently found abnormal immunological reactions towards new antigens and a chronic inflammatory state that predisposes to a response oriented towards the Th2 cytokinic pattern, allowing allergens to penetrate into tissues. Nevertheless, in the elderly drug related allergic disorders, in particular at the skin level, are often underdiagnosed and difficult to treat.Among the IgE mediated cases, urticaria and angioedema are frequent. However, there are also cell-mediated mechanisms; in particular delayed type reactions to drugs often arise in the elderly for the reiterate use of topical medications (anesthetic, antibiotic and anti-inflammatory creams).A detailed anamnestic history is essential to establish the causal link between an adverse drug reaction and the specific drug. Moreover, in some cases it is necessary the specific knowledge of the histological picture
Atypical hand foot and mouth disease related Coxsackievirus-A10 infection in an adult patient
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Emphysematous cystitis in an elderly male diabetic patient: a case report. A complicated urinary tract infection
No full textEmphysematous Cystitis (EC) is a rare form of complicated urinary tract infection, characterized by the presence of gas in the bladder and in its wall. Diabetes Mellitus (DM) is the major risk factor. Clinical manifestations in EC can range from asymptomatic form to severe sepsis and fatal events. Abdominal imaging is requested to diagnose EC and evaluate its severity. Early diagnosis and appropriate management improve the outcome. We report a case of incidental EC diagnosis in an 82-year-old diabetic male who was investigated for severe anemia
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