55 research outputs found

    Evaluation of Antioxidant Properties, Elemental and Phenolic Contents Composition of Wild Nettle (Urtica Dioica L.) from Tunceli in Turkey

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    Evaluation of antioxidant properties, elemental and phenolic contents composition of wild nettle (Urtica dioica L.) from Tunceli in Turkey. (PMID:24199953) Abstract Citations Related Articles Data BioEntities External Links Yildirim NC 1 , Turkoglu S , Ince OK , Ince M Affiliations Cellular and Molecular Biology (Noisy-le-Grand, France) [03 Nov 2013, Suppl 59:OL1882-8] Type: Journal Article Abstract Wild nettle (Urtica dioica L.) types were sampled from different geographical regions in Tunceli (Turkey) to determine their mineral, vitamin, phenolic contents and their antioxidant properties. The total phenol varied from 37.419 ± 0.380 to 19.182 ± 1.00 mg of GAEs g(-1) of dry nettle. The highest radical scavenging effect was observed in Mazgirt parting of the ways 7.5 km with 33.70 ± 0.849 mg mL(-1). The highest reducing power was observed in the nettles from Mazgirt parting of the ways 7.5 km. Among the various macronutrients estimated in the plant samples, potassium was present in the highest quantity followed by calcium and phosphate. Kaempferol and resveratrol were not determined in some nettle samples but rutin levels were determined in all samples. Vitamin A concentrations were ranged between 13.64 ± 1.90 and 5.74 ± 1.00 (mg kg(-1) dry weight). These results show that Urtica dioica L. collected from Tunceli in Turkey could be considered as a natural alternative source for food, pharmacology and medicine sectors

    Novel Hybrid Scaffolds for the Cultivation of Osteoblast Cells

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    Turkoglu Sasmazel, Hilal/0000-0002-0254-4541In this study, natural biodegradable polysaccharide, chitosan, and synthetic biodegradable polymer, poly(epsilon-caprolactone) (PCL) were used to prepare 3D, hybrid polymeric tissue scaffolds (PCL/chitosan blend and PCL/chitosan/PCL layer by layer scaffolds) by using the electrospinning technique. The hybrid scaffolds were developed through HA addition to accelerate osteoblast cell growth. Characteristic examinations of the scaffolds were performed by micrometer, SEM, contact angle measurement system, ATR-FTIR, tensile machine and swelling experiments. The thickness of all electrospun scaffolds was determined in the range of 0.010 +/- 0.001-0.012 +/- 0.002 mm. In order to optimize electrospinning processes, suitable bead-free and uniform scaffolds were selected by using SEM images. Blending of PCL with chitosan resulted in better hydrophilicity for the PCL/chitosan scaffolds. The characteristic peaks of PCL and chitosan in the blend and layer by layer nanofibers were observed. The PCL/chitosan/PCL layer by layer structure had higher elastic modulus and tensile strength values than both individual PCL and chitosan structures. The layer by layer scaffolds exhibited the PBS absorption values of 184.2; 197.2% which were higher than those of PCL scaffolds but lower than those of PCL/chitosan blend scaffolds. SaOs-2 osteosarcoma cell culture studies showed that the highest ALP activities belonged to novel PCL/chitosan/PCL layer by layer scaffolds meaning better cell differentiation on the surfaces. (C) 2011 Elsevier B.V. All rights reserved.Turkish Academy of Science (TUBA) L'Oreal; L'OrealThe author is greatly thankful to Turkish Academy of Science (TUBA) & L'Oreal for honoring this study with the award "Young Women in Science" in Materials Science in 2009. Her special thanks also go to L'Oreal for the precious financial support. The author also appreciates the invaluable contribution of AWAC (Academic Writing Advisory Center) to this study in linguistic terms

    Turkish truffles I: 18 new records for Turkey

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    WOS: 000352486200014We report the first records of 18 truffle species in Turkey. Three belong to the Ascomycota: Elaphomyces leucocarpus, E. muricatus, and Genea sphaerica; and 15 to the Basidiomycota: Alpova corsicus, Gautieria otthii, G. retirugosa, G. trabutii, Hymenogaster citrinus, H. hessei, H. luteus, H. lycoperdineus, Hysterangium clathroides, H. epiroticum, H. fragile, H. nephriticum, Leucogaster tozzianus, Octaviania asterosperma, and Protoglossum aromaticum. We also report new localities within Turkey for Picoa juniperi, P. lefebvrei, Geopora cooperi, Terfezia arenaria, T. claveryi, Tuber aestivum, and T. nitidum in the Ascomycota; and Leucogaster nudus, Hymenogaster thwaitesii, H. vulgaris, and Melanogaster broomeanus in the Basidiomycota.Scientific and Technological Research Council of Turkey projectTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [T-BAG-111T530, BIDEB-2221]The first author received funding from the Scientific and Technological Research Council of Turkey project number T-BAG-111T530 and BIDEB-2221. We appreciate the help from Abdulkadir Simsek, Ahmet Oksuzoglu, Cemhan Bucak, Coskun Bilgi, Duran Celik, Ekrem Toprak, Esra Er, Fatih Kaya, Gulsum Turkoglu, Idris Sener, Kadir Bazlica, Kadir Ceryan, Mehmet Halil Solak, Mehmet Metin, Mehmet Yucel, Murat Kilic, Mustafa Demir, Mustafa Turuncoglu, Niyazi Ulucoban, Okan Kursun, Osman Coban, Serkan Sevinc, Seyit Ahmet Akay, Tolga Keser, Ugur Demirbilek, Veysel Kodalak, and Yavuzalp Turkoglu in the collection of some of the specimens

    A THERMO-STABLE DEHALOGENASE IN THE EXTRACTS FROM PSEUDOMONAS SP (STRAIN-19S)

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    A thermo-stable dehalogenase was demonstrated in the crude extracts from Pseudomonas sp (19S). The ability of the enzyme to catalyze the dehalogenation of various halogen-substituted organic acids was investigated and the highest activity was found with monochloroacetate. The enzyme followed Michael is-Menten kinetics, and the Km for monochloroacetate was O.2mM. Maximum activity was found at pH 10.5 and 60~ The enzyme activity in the cell-free extract was unaffected by EDTA or by Mn, Zn, or Cu ions, but was dramatically reduced by HgClz (70 %) and Pb (N03)~ (80 %)

    Is ischemia-modified albumin a reliable tool for the assessment of acute pancreatitis?

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    Abdurrahman Sahin,1 Semra Turkoglu,2 Nurettin Tunc,1 Deccane Duzenci,3 Ozgen Arslan Solmaz,4 Ibrahim Halil Bahcecioglu,1 Mehmet Yalniz1 1Medicine Faculty, Department of Gastroenterology, Firat University, Elazig, Turkey; 2Department of Nutrition and Dietetics, Health Sciences Faculty, Firat University, Elazig, Turkey; 3Department of Internal Medicine, Elazig Education and Training Hospital, Elazig, Turkey; 4Department of Pathology, Elazig Education and Training Hospital, Elazig, Turkey Purpose: Oxidative stress has been implicated in several disorders, including acute pancreatitis (AP). Ischemia-modified albumin (IMA), which reflects the ability to bind cobalt, has been found to be elevated in conditions of oxidative stress and tissue hypoxia. This study examined IMA and adjusted IMA levels in patients with AP, and examined the associations of IMA and adjusted IMA levels to the severity of AP.Patients and methods: A total of 42 consecutive patients with AP and 43 age- and sex-matched control subjects were enrolled. Serum samples were obtained from patients with AP on admission as well as 48–72 hours after hospitalization, and from the controls, at the time of enrollment. Adjusted IMA was calculated by multiplying the IMA value of each patient with the ratio of the patient’s albumin value and the median albumin value of the study population. The severity of AP was assessed according to the modified Atlanta classification, and the patients were divided into 2 groups: mild AP and severe AP.Results: The serum IMA and adjusted IMA values of patients with AP on admission and those of the controls did not differ (p=0.86 and p=0.99, respectively). The second measurements of IMA and adjusted IMA in the AP group were higher than the first measurements of both the AP group and controls (for all, p<0.01). Among the IMA measurements, only adjusted IMA on admission had the ability to predict the severity of AP. Severe AP was correlated with albumin, and the area under the curve of adjusted IMA values on admission was 0.746 for differentiating patients with severe AP from mild AP with statistical significance (p=0.005).Conclusion: It was shown that IMA and adjusted IMA levels rise with the progression of AP. Lower levels of adjusted IMA predict the severity of AP. Further studies with serial measurements of IMA are warranted to explore the indicative role of IMA in the course of AP. Keywords: ischemia modified albumin, adjusted ischemia modified albumin, acute pancreatitis, severit

    ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC AND FLAVONOID CONTENT OF WATER AND ETHANOL EXTRACTS FROM ACHILLEA MILLEFOLIUM L

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    Achillea millefolium (Asteraceae, civanperçemi) deri iltihaplanmaları, spazmodik ve gastrointestinal bozukluklar gibi çeşitli rahatsızlıklara karşı halk ilacı olarak kullanılmıştır. Bu çalışmada, etanol ve su ekstreleri A. millefolium çiçek, yaprak ve tohumlarından hazırlandı. Antioksidan aktivite, ferik tiyosiyanat metodu ve H2O2 radikal temizleme testleriyle ölçüldü ve A. millefolium ekstrelerinin fenolik bileşikleri ve flavonoit içerikleri saptandı. Sonuç olarak, A. millefolium çiçek, yaprak ve tohumları etkili H2O2 radikal temizleme aktivitesi ve lipid peroksidasyon inhibisyonu aktivitesi gösterdi ve bu antioksidan aktiviteler referans antioksidanlar BHA ve ?-tokoferol ile karşılaştırıldı.Achillea millefolium (Asteraceae, yarrow) has been used in folk medicine against several disturbances including skin inflammations, spasmodic and gastrointestinal disorders. In this study, ethanol and water extracts were prepared from A. millefolium flowers, leaves and seeds. Antioxidant activities were measured by ferric thiocyanate method, and H2O2radical scavenging activity assays and phenolic compounds and flavonoid contents of A. millefolium extracts were also determined. In conclusion, extracts of A. millefolium flowers, leaves and seeds had effective H2O2radical scavenging activity, total antioxidant activity, and these antioxidant activities were compared with BHA and ?tocopherol as reference antioxidant

    The effect of Pistacia terebinthus extract on lipid peroxidation, glutathione, protein, and some enzyme activities in tissues of rats undergoing oxidative stress

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    This study measures the effects of Pistacia terebinthus flower extract on the amounts of lipid peroxidation (LPO), total protein, glutathione, and enzyme activities in blood. It also measures the effects of the plant extract on glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) enzyme activities and it arrives at the conclusion that GSH-Px activity significantly increased in the Pistacia terebinthus-hydrogen peroxide group (P < 0.0001) and SOD activity meaningfully increased in the Pistacia terebinthus (P < 0.05) and Pistacia terebinthus-hydrogen peroxide (P < 0.0001) groups. It is also found that P. terebinthus extract has protective effects against LPO exerted in the kidney, brain, and lungs (P < 0.0001). The positive effects of P. terebinthus extract are also found on the levels of glutathione and total protein in all organs, except for the level of glutathione in the spleen and brain, and the increase of glutathione and total protein (P < 0.0001). These results are parallel to the effect of the extract used in this study on the LPO levels in tissues and they show that the plant species used in the study has positive effects on the activities of antioxidant molecules as well as protective effects against LPO

    Determination of Antioxidant Capacities of Phlomis pungens Wild. var. hispida Hub.-Mor.

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    WOS: 000305033900091The genus Phlomis L. belongs to the Lamiaceae family and encompasses 100 species native to Turkey, North Africa, Europe and Asia. It is a popular herbal tea enjoyed for its taste and aroma. Phlomis species are used to treat various conditions such as diabetes, gastric ulcer, hemorrhoids, inflammation and wounds. In this study, ethanol and water extracts were prepared from powdered P pungens flowers and leaves. Antioxidant activities were measured by 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(center dot+)) radical scavenging, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH center dot) scavenging, superoxide anion (02) radical scavenging, reducing activity and ferrous metal chelating activity assays. In conclusion, P. pungens flowers and leaves had effective ABTS(center dot+) and DPPH center dot radical scavenging, superoxide anion radical scavenging, reducing power and metal chelating activities when compared with BHA, BHT and alpha-tocopherol as reference antioxidants
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