578 research outputs found
sciseim/GHSROS_MS: Supplemental information, code, and data for the MS: The long non-coding RNA GHSROS promotes tumor growth and mediates expression of genes associated with metastasis and adverse disease outcome.
No full textGHSROS_MS
<p>Supplemental information, code, and data for the MS: Patrick B. Thomas, Inge Seim, Eliza Whiteside, Carina Walpole, Michelle Maugham, Lidija Jovanovic, Jennifer H. Gunter, Colleen C. Nelson, Adrian C. Herington, Rakesh Veedu, Penny L. Jeffery, Lisa K. Chopin. <strong>The long non-coding RNA <em>GHSROS</em> promotes tumor growth and mediates expression of genes associated with metastasis and adverse disease outcome</strong>.</p>
The Neighbourhood of Infinity
No full textFanzine dedicated to the work of Mark E Smith and The Fall. Collaboration between myself and artists, Inge Marleen and David Powell. Sole author of text:
“And then I heard a voice say, ‘Hey, you’re lost in music.’
George B. Inge papers, MSS.0728
No full textAbstract: Research material for the book, The Herndon and Inge Families: Genealogical, Historical, Biographical.Scope and Content Note: This collection contains research material for the book, The Herndon and Inge Families: Genealogical, Historical, Biographical, written by Inge and published by the Gregath Company of Cullman, Alabama, in 1977. The papers include correspondence regarding the book, correspondence from Inge family members, note cards, handwritten notes, newspaper clippings, excerpts from books containing genealogical information, and drafts of the manuscripts.Biographical/Historical Note: Colonel George B. Inge was born and raised in Mobile, Alabama. Inge served in the U. S. Army Corps of Engineers Reserve during World War II and worked for many years in the United States Civil Service prior to his retirement in 1962. He has been honored with numerous military medals and ribbons and has been a member and leader in many civic organizations in Mobile. In addition to The Herndon and Inge Families: Genealogical, Historical, Biographical, Inge is also the author of Our Book of State, a history of the Order of Myths, Mobile's oldest parading Mardi Gras society. He is married to Marie Bishop Inge. Information obtained from The Herndon and Inge Families: Genealogical, Historical, Biographical
A re-examination of the Ghrelin and Ghrelin receptor genes
No full textThe last few years have seen dramatic advances in genomics, including the discovery of a large number of non-coding and antisense transcripts. This has revolutionised our understanding of multifaceted transcript structures found within gene loci and their roles in the regulation of development, neurogenesis and other complex processes. The recent and continuing surge of knowledge has prompted researchers to reassess and further dissect gene loci. The ghrelin gene (GHRL) gives rise to preproghrelin, which in turn produces ghrelin, a 28 amino acid peptide hormone that acts via the ghrelin receptor (growth hormone secretagogue receptor/GHSR 1a). Ghrelin has many important physiological and pathophysiological roles, including the stimulation of growth hormone (GH) release, appetite regulation, and cancer development. A truncated receptor splice variant, GHSR 1b, does not bind ghrelin, but dimerises with GHSR 1a, and may act as a dominant negative receptor. The gene products of ghrelin and its receptor are frequently overexpressed in human cancer While it is well known that the ghrelin axis (ghrelin and its receptor) plays a range of important functional roles, little is known about the molecular structure and regulation of the ghrelin gene (GHRL) and ghrelin receptor gene (GHSR). This thesis reports the re-annotation of the ghrelin gene, discovery of alternative 5’ exons and transcription start sites, as well as the description of a number of novel splice variants, including isoforms with a putative signal peptide. We also describe the discovery and characterisation of a ghrelin antisense gene (GHRLOS), and the discovery and expression of a ghrelin receptor (growth hormone secretagogue receptor/GHSR) antisense gene (GHSR-OS). We have identified numerous ghrelin-derived transcripts, including variants with extended 5' untranslated regions and putative secreted obestatin and C-ghrelin transcripts. These transcripts initiate from novel first exons, exon -1, exon 0 and a 5' extended 1, with multiple transcription start sites. We used comparative genomics to identify, and RT-PCR to experimentally verify, that the proximal exon 0 and 5' extended exon 1 are transcribed in the mouse ghrelin gene, which suggests the mouse and human proximal first exon architecture is conserved. We have identified numerous novel antisense transcripts in the ghrelin locus. A candidate non-coding endogenous natural antisense gene (GHRLOS) was cloned and demonstrates very low expression levels in the stomach and high levels in the thymus, testis and brain - all major tissues of non-coding RNA expression. Next, we examined if transcription occurs in the antisense orientation to the ghrelin receptor gene, GHSR. A novel gene (GHSR-OS) on the opposite strand of intron 1 of the GHSR gene was identified and characterised using strand-specific RT-PCR and rapid amplification of cDNA ends (RACE). GHSR-OS is differentially expressed and a candidate non-coding RNA gene. In summary, this study has characterised the ghrelin and ghrelin receptor loci and demonstrated natural antisense transcripts to ghrelin and its receptor. Our preliminary work shows that the ghrelin axis generates a broad and complex transcriptional repertoire. This study provides the basis for detailed functional studies of the the ghrelin and GHSR loci and future studies will be needed to further unravel the function, diagnostic and therapeutic potential of the ghrelin axis
Uncloseting Drama: Tennessee Williams, William Inge, and Gay Identity in Terry Teachout's Billy and Me
No full textThis paper was presented at the 39th Annual William Inge Theater Festival & Conference hosted by the William Inge Center for the Arts in April 2022.Terry Teachout’s 2017 play, Billy and Me, imagines two fictional encounters between Tennessee Williams and William Inge: first, in a bar in Chicago in 1944 immediately following a pre-Broadway tryout of The Glass Menagerie, then in New York in 1959 following the premiere of Inge’s A Loss of Roses. Through fictional dialogue, Teachout builds upon the historical relationship between these two playwrights to imagine the conversations that must have connected them as two midcentury gay playwrights in America: success and failure, sexual conquests, relationships, and addiction. In this way, Teachout’s play attempts to “uncloset” the issues that were at the heart of Williams’ and Inge’s life and work. Through a comparative analysis of specific characters and situations in their plays, this paper explores how the representation of white, gay male identity varies from the closet dramas of Williams and Inge to the uncloseted and celebrated representation of sexual identity in the theatre of today.
Teachout was the lead drama critic for The Wall Street Journal, playwright of Satchmo at the Waldorf, and author or editor of nearly eight books until his untimely death in 2022. His passion and respect for the writing and craft of America’s midcentury playwrights is apparent in the text of Billy and Me, which has had three productions up until now, providing an interesting study in how this work revivifies its historical subjects through both content as well as form
Inge Lehmann’s work materials and seismological epistolary archive
No full text<p style="margin: 0.0px 0.0px 0.0px 0.0px; font: 9.0px Times;"> </p> <p style="margin: 0.0px 0.0px 0.0px 0.0px; font: 9.0px Times;">The Inge Lehmann archive contains thousands of seismological work documents from Inge Lehmann’s private home. For a long time the author thought that the main concern was to keep the documents for posterity. There is now a renewed interest in Inge Lehmann, and some documents were presented in a poster at ESC Potsdam 2004, and the collection of documents were scanned and catalogued 2005-2006 at Storia Geofisica Ambiente in Bologna. Inge Lehmann (1888-1993) is famous for her discovery in 1936 of the earth’s inner core and for work on the upper mantle. A short biography is given. After her retirement in 1953 she worked at home in Denmark, and abroad in USA and in Canada. She took part in the creation of the European Seismological Commission in 1951, and in the creation of the International Seismological Centre in 1964. Inge Lehmann received many awards. Some letters from her early correspondence with Harold Jeffreys are discussed, they show how the inner core was discussed already in 1932. A few of the author’s reminiscences of Inge Lehmann are given.</p> <br />
Turtle ghrelin
No full textIn a recent paper, Wang and colleagues described the genomes of two turtles, the Chinese soft-shell turtle (Pelodiscus sinensis) and the green sea turtle (Chelonia mydas)1. A salient finding was an apparent absence of GHRL, the gene encoding the only known circulating orexigen, the peptide hormone ghrelin. The highly conserved GHRL encodes at least two bioactive peptide hormones, ghrelin2 and obestatin3, which are recognized to have a diverse range of functions in a number of cell types and physiological systems4, 5. Wang and colleagues hypothesized that the absence of ghrelin was associated with the low metabolic rate observed in these turtle species1
Tandem B1 SINE retro-elements may provide a basis for natural antisense transcription in the Magi1 locus of the mouse (Mus musculus)
No full textTransposable elements, which are DNA sequences that can move between different sites in genomes, comprise approximately 40% of the genome of mammals and are emerging as important contributors to biological diversity. Here we report a transcription unit lying within intron 1 of the murine Magi1 (membrane associated guanylate kinase inverted 1) gene that codes for a cell-cell junction scaffolding protein. The transcription unit, termed Magi1OS (Magi1 Opposite Strand), originates from a region with tandem B1 short interspersed nuclear elements (SINEs) and is an antisense gene to Magi1. Mag1OS transcription initiates in a proximal B1 element that shows only 4% divergence from the consensus sequence, indicating that it has been recently inserted into the mouse genome and could be replication competent. Moreover, a chimaeric transcript may result from intra-chromosomal interaction and trans-splicing of the Magi1 antisense transcript (Magi1OS) and Ghrl, which codes for the multifunctional peptide hormone ghrelin. These two genes are 20 megabases apart on chromosome 6 and are transcribed in opposite directions. We propose that the Magi1OS locus may serve as a useful model system to study exaptation and retrotransposition of B1 SINEs, as well as to examine the mechanisms of intra-chromosomal trans-splicing
Gene expression signatures of human cell and tissue longevity
No full textDifferent cell types within the body exhibit substantial variation in the average time they live, ranging from days to the lifetime of the organism. The underlying mechanisms governing the diverse lifespan of different cell types are not well understood. To examine gene expression strategies that support the lifespan of different cell types within the human body, we obtained publicly available RNA-seq data sets and interrogated transcriptomes of 21 somatic cell types and tissues with reported cellular turnover, a bona fide estimate of lifespan, ranging from 2 days (monocytes) to a lifetime (neurons). Exceptionally long-lived neurons presented a gene expression profile of reduced protein metabolism, consistent with neuronal survival and similar to expression patterns induced by longevity interventions such as dietary restriction. Across different cell lineages, we identified a gene expression signature of human cell and tissue turnover. In particular, turnover showed a negative correlation with the energetically costly cell cycle and factors supporting genome stability, concomitant risk factors for aging-associated pathologies. In addition, the expression of p53 was negatively correlated with cellular turnover, suggesting that low p53 activity supports the longevity of post-mitotic cells with inherently low risk of developing cancer. Our results demonstrate the utility of comparative approaches in unveiling gene expression differences among cell lineages with diverse cell turnover within the same organism, providing insights into mechanisms that could regulate cell longevity
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