1,720,966 research outputs found
NEW APPLICATION OF AFFINITY CHROMATOGRAPHY - IDENTIFICATION AND STUDY OF SPECIFIC CARRIER PROTEINS
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POWERFUL INHIBITOR OF GUANINE DEAMINASE
No full textThe synthesis of 9-(p-carbetoxyphenyl) guanine is reported. The assays carried out on guanine deaminase from rat and rabbit liver and pig brain show that this compound is a powerful inhibitor. The compound has a Ki = 5 uM for the enzyme from pig brain. The use of the inhibitor for the synthesis of a specific adsorbent for guanine deaminase was studied
PRELIMINARY INVESTIGATIONS ON PLASMA-BINDING OF URATE BY MEANS OF AFFINITY CHROMATOGRAPHY
No full textEFFECTS OF SUBSTITUTED 1,2,3-TRIAZOLES ON ADENOSINE-DEAMINASE, GUANINE DEAMINASE AND XANTHINE-OXIDASE
No full textThe action of some known and new synthesized substituted 1,2,3-triazoles on adenosine deaminase, guanine deaminase and xanthine oxidase was studied. The effect of substituents in 1, 4 and 5 positions was studied and discussed. The presence of a carboxamido group in 4 position seems to be essential in the binding to adenosine deaminase
AFFINITY LABELING OF HISTIDINE AND LYSINE RESIDUES IN THE ADENOSINE-DEAMINASE SUBSTRATE BINDING-SITE
No full text1. Adenosine deaminase was inactivated by 9-(4-bromoacetamidobenzyl)-adenine (I) and 9-(2-bromoacetamidobenzyl)adenine (II), two affinity labels. 2. The stoichiometry of the reaction with reagent II is reported: 1 mol reagent is bound per mol inactive enzyme. Amino acid analysis of the 6 N HCl hydrolyzate of the inactive enzyme identified CM-histidine as the main alkylation product. This is the first evidence of the presence of a histidine in the active site region. 3. The alkylation rate and involved amino acid residues were studied for both reagents I and II, at pH 8 and 5.5. The particular reactivity of a lysine near or in the active site is discussed
EVIDENCE OF SOLUBLE-PROTEINS BINDING ADRIAMYCIN BY AFFINITY CHROMATOGRAPHY
No full textThe presence of various tissues of soluble proteins binding adriamycin is evidenced by affinity chromatography
EFFECTS OF 6,9-DISUBSTITUTED 8-AZAPURINES ON ADENOSINE-DEAMINASE, GUANINE DEAMINASE AND XANTHINE-OXIDASE
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