1,739,210 research outputs found

    Jun N-terminal kinase 1 (JNK1) as a molecular target to limit cellular mortality under hypoxia

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    Many pathological conditions and environmental impacts lead to a decrease in tissue oxygen supply and severe cellular hypoxia. This secondary hypoxia can disturb cellular homeostasis, limiting the efficacy of the prescribed treatment for the primary disease, eventually leading to cellular and organismal death. Jun N-terminal kinase 1 (JNK1) plays a central role in the development of cellular damage under hypoxia, hypoxia/reoxygenation and ischemia/reperfusion conditions. Therefore, we selected JNK1 protein as a molecular target to limit cellular damage and death during hypoxia. The primary objective of this research was to study the influence of the suppression of JNK1 on the development of cellular hypoxic damage. It was hypothesized that suppression of JNK1 will decrease cellular mortality under hypoxia and may increase the efficacy of traditional treatment of many pathological conditions. The hypothesis was verified under both in vitro and in vivo conditions. Another objective was to develop a non-viral system for intracellular delivery of antisense oligonucleotides (ASO) and small interfering RNA (siRNA). ASO and siRNA targeted to JNK1 mRNA delivered by neutral and cationic liposomes, respectively, showed high efficiency in suppressing JNK1 protein. Such suppression limited the caspase dependent apoptosis signaling pathway and decreased cellular mortality induced by severe hypoxia. The results suggest that JNK1 protein might be an attractive target for antihypoxic therapy to increase resistance to many pathological conditions and diseases accompanied by cellular hypoxia.Ph.D.Includes bibliographical references (p. 138-153)by Seema S. Betiger

    The Haunting of Seema: a Supernatural Struggle

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    One evening Belahur's daughter Seema went outside to fetch water. She felt something strange but ignored it and carried the water home. Later, she went to the shop along the same path to get a tobacco dip (nasw?r, a moist, powdered tobacco used in Pakistan and Afghanistan) for her father. This time, she saw an ugly man with long hair near a big oak tree. This tree was known to be haunted by a jinn who appeared in human form and possessed people. So, people usually avoided passing by this tree especially in the evening. When Seema returned home, her voice had changed to that of an old man, and her hands were shivering. When her mother, Belahur, asked what was going on Seema replied in a deep voice: I am not alone. We are three, and the other two are girls. All of us liked your daughter and want to take her. Belahur, terrified and desperate, tried to choke Seema, thinking she was squeezing the neck of the man possessing her. Seema reacted violently, crying, beating herself, and behaving unusually. Belahur's husband decided to take Seema to a religious scholar molvi, for help. Using his knowledge and skills, the molvi managed to expel the [bad] spirits from Seema and made them promise never to return. He explained that the jinns were non-Muslim supernatural beings who always tried to harm their victims. However, despite the molvi's intervention, Seema began to show unusual symptoms again, indicating that the supernatural forces continued to have an influence on her.2.3.2.

    READ Poster: Seema Kakade

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    Professor Seema Kakade recommends Lawrence Susskind, Paul F. Levy, and Jennifer Thomas\u27s Negotiating Environmental Agreements: How to Avoid Escalating Confrontation, Needless Costs, and Unnecessary Litigation.https://digitalcommons.law.umaryland.edu/read/1051/thumbnail.jp

    Ceremony - Seema Patel, Nicholas Petrovski, Justin Poh

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    Graduates Seema Patel, Nicholas Petrovski, and Justin Poh receive their hoods.https://scholarship.kentlaw.iit.edu/commencement_2013/1146/thumbnail.jp

    The Post Office Horizon system and Seema Misra

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    The author highlights the significance of the Seema Misra case in raising questions about the reliablity of the Post Office Horizon system and more widely suggesting that all digital systems have the possibility of latent defects, and these can never be discounted. He argues that when the efficacy of digital systems is called into question in legal proceedings, the onus of proof must be placed on the supplier of these systems and not the accuser.Index words: Post Office; Horizon; prosecutions; software errors; disclosure Full transcript of the trial Regina v Seema Misra, T2009007 (England & Wales; theft; electronic evidence; Post Office Horizon System; ‘reliability’ of computers) with case commentary and index to original papers held in the Documents Supplement of Volume 12: 2015
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