17 research outputs found
Molecular epidemiology of canine parvovirus in Morocco
No full textSince it first emergence in the mid-1970's, canine parvovirus 2 (CPV-2) has evolved giving rise to new antigenic variants termed CPV-2a, CPV-2b and CPV-2c, which have completely replaced the original strain and had been variously distributed worldwide. In Africa limited data are available on epidemiological prevalence of these new types. Hence, the aim of the present study was to determine circulating variants in Morocco. Through TaqMan-based real-time PCR assay, 91 samples, collected from symptomatic dogs originating from various cities between 2011 and 2015, were diagnosed. Positive specimens were characterised by means of minor groove binder (MGB) probe PCR. The results showed that all samples but one (98.9%) were CPV positive, of which 1 (1.1%) was characterised as CPV-2a, 43 (47.7%) as CPV-2b and 39 (43.3%) as CPV-2c. Interestingly, a co-infection with CPV-2b and CPV-2c was detected in 4 (4.4%) samples and 3 (3.3%) samples were not characterised. Sequencing of the full VP2 gene revealed these 3 uncharacterised strains as CPV-2c, displaying a change G4068A responsible for the replacement of aspartic acid with asparagine at residue 427, impacting the MGB probe binding. In this work we provide a better understanding of the current status of prevailing CPV strains in northern Africa
Impact of the initialisation on population balance CFD models coupled with two-phase flow
No full text
Crystal structure, Hirshfeld surface analysis and interaction energy, DFT and antibacterial activity studies of ethyl 2-[(2Z)-2-(2-chlorobenzylidene)-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-4-yl]acetate
No full textThe title compound, C19H16ClNO3S, consists of chlorophenyl methylidene and dihydrobenzothiazine units linked to an acetate moiety, where the thiazine ring adopts a screw-boat conformation. In the crystal, two sets of weak C-HPh center dot center dot center dot ODbt (Ph = phenyl and Dbt = dihydrobenzothiazine) hydrogen bonds form layers of molecules parallel to the bc plane. The layers stack along the aaxis direction with intercalation of the ester chains. The crystal studied was a two component twin with a refined BASF of 0.34961 (5). The Hirshfeld surface analysis of the crystal structure indicates that the most important contributions to the crystal packing are from H center dot center dot center dot H (37.5%), H center dot center dot center dot C/C center dot center dot center dot H (24.6%) and H center dot center dot center dot O/O center dot center dot center dot H (16.7%) interactions. Hydrogen-bonding and van der Waals interactions are the dominant interactions in the crystal packing. Computational chemistry indicates that in the crystal, C-H-Ph center dot center dot center dot O-Dbt hydrogen bond energies are 38.3 and 30.3 kJ mol(-1). Density functional theory (DFT) optimized structures at the B3LYP/ 6-311 G(d,p) level are compared with the experimentally determined molecular structure in the solid state. The HOMO-LUMO behaviour was elucidated to determine the energy gap. Moreover, the antibacterial activity of the title compound has been evaluated against gram-positive and gram-negative bacteria
Crystal structure, Hirshfeld surface analysis and interaction energy, DFT and antibacterial activity studies of (Z)-4-hexyl-2-(4-methylbenzylidene)-2H-benzo[b][1,4]thiazin-3(4H)-one
No full textThe title compound, C22H25NOS, consists of methylbenzylidene and benzothiazine units linked to a hexyl moiety, where the thiazine ring adopts a screw -boat conformation. In the crystal, inversion dimers are formed by weak C Hmthn' " OBnzthz hydrogen bonds and are linked into chains extending along the a -axis direction by weak C H- -Bnz" " " OBnzthz (Bnz = benzene, Bnzthz = benzothiazine and Mthn = methine) hydrogen bonds. A Hirshfeld surface analysis of the crystal structure indicates that the most important contributions for the crystal packing are from H" " "H (59.2%) and H" " -C/C- " "H (27.9%) interactions. Hydrogen bonding and van der Waals interactions are the dominant interactions in the crystal packing. Computational chemistry indicates that in the crystal, the C HBnz" " " OBnzthz and C HMthn. " " OBnzthz hydrogenbond energies are 75.3 and 56.5 kJ mol-1, respectively. Density functional theory (DFT) optimized structures at the B3LYP/ 6-311 G(d,p) level are compared with the experimentally determined molecular structure in the solid state. The HOMO LUMO behaviour was elucidated to determine the energy gap. Moreover, the antibacterial activity of the title compound was evaluated against gram -positive and gram-negative bacteria
Isolation and identification of group A rotaviruses among neonatal diarrheic calves, Morocco
No full textProfil moléculaire et épidémiologique du virus de la bursite infectieuse aviaire circulant au Maroc entre 2013 et 2016
No full textGumboro disease or infectious bursal disease (IBD) is associated with the infectious bursal disease virus (IBDV), which causes considerable economic losses. This virus belongs to the family Birnaviridae (Avibirnavirus). This infection continues to be prevalent in poultry farms in Morocco, particularly in broiler farms, despite the various vaccination programs applied. Thus, the objective of the present work is the epidemiological and pathotypic characterization of IBDV in Morocco. The study involved 102 suspect broiler farms throughout Morocco surveyed between 2013 and 2016. As well as Fabricius Bursa (FB) samples (pool of 5 BF per flock) were taken from affected chickens for molecular investigations by real-time RT-PCR. The RT-PCR used allowed both detection and discrimination between highly virulent IBDV (vvIBDV) and non-vvIBDV strains using allelic discrimination probes. The epidemiological investigation revealed the suspicion of IBDV in poultry farms due to the very characteristic clinical and necroptic signs caused by IBDV (apathy, ruffled feathers, prostration, watery whitish diarrhea, and the necrotic triad (hypertrophy and hemorrhage of the Fabricius bursa; kidney congestion and petechiae in the muscles). The suspicion was confirmed by the results of RT-PCR in all regions of the kingdom with a prevalence of overall IBDV infection of 81% of which 60% for classical strains (non-vvIBDV) and 40% for highly virulent strains (vvIBDV).
Keywords: Infectious bursal disease, IBDV, RT-PCR, Epidemiology, MoroccoLa maladie de Gumboro ou bursite infectieuse aviaire (IBD) est associée au virus de la bursite infectieuse (IBDV), qui cause des pertes économiques considérables. Ce virus appartient à la famille des Birnaviridae (Avibirnavirus). Cette infection continue à sévir au sein des élevages avicoles au Maroc particulièrement ceux de poulets de chair malgré les différents programmes de vaccination appliqués. Ainsi, l’objectif du présent travail est la caractérisation épidémiologique et pathotypique de l’IBDV au Maroc102 élevages de poulets de chair suspects à travers tout le Maroc enquêtés entre les années 2013 et 2016. Ainsi que les bourses de Fabricius (BF) (pool de 5 BF par élevage) ont été prélevés à partir de poulets affectés pour des investigations moléculaires par RT-PCR en temps réel. La RT-PCR utilisée permettait aussi bien la détection que la discrimination entre les souches très virulentes de l’IBDV (vvIBDV) et les non-vvIBDV en utilisant des sondes de discrimination allélique. L\u27enquête épidémiologique révèle la suspicion de l’atteinte des élevages avicoles par l’IBDV vu les signes cliniques et nécropsiques très caractéristiques causés par l’IBDV (apathie, plumes ébouriffées, prostration, diarrhée blanchâtre aqueuse, et la triade nécrosique (hypertrophie et hémorragie de la Bourse de Fabricius ; congestion des reins et pétéchie au niveau des muscles)). La suspicion a été confirmée par les résultats de la RT-PCR dans toutes les régions du royaume avec une prévalence de l’atteinte par l’IBDV globale de 81% dont 60% pour les souches classiques (non-vvIBDV) et 40% pour les souches très virulentes (vvIBDV).
Mots clés: Bursite infectieuse, IBDV, RT-PCR, épidémiologie, Maro
New 1,2,3-triazole containing benzimidazolone derivatives: Syntheses, crystal structures, spectroscopic characterizations, Hirshfeld surface analyses, DFT calculations, anti-corrosion property anticipation, and antibacterial activities
No full textA new series of 1,4 and 1,5-disubstituted-1,2,3-triazole derivatives containing a benzimidazolone moiety [(3a-3b), (4a-4b), (5a-5b) and (6a-6b)] were synthesized using 1,3-dipolar cycloaddition in toluene under thermal conditions with N-alkyl, N-propargyl- benzimidazolones as dipolarophiles and azide derivatives as dipoles. Furthermore, the 1,4-disubstituted-1,2,3-regioisomers (3a-6a) have been also obtained exclusively using click chemistry (Copper-Catalyzed Azide-Alkyne Cycloaddition). The structures of all compounds were characterized by (1) H-and C-13-NMR spectroscopy. The molecular and crystal structures of three compounds (3a, 4a, and 5a) were confirmed by single crystal X-ray crystallography. In addition, Density Functional Theory (DFT) was used to predict spectral data at the B3LYP/6-31G (d, p) level. Intermolecular interactions in the crystals of 3a, 4a, and 5a were determined by Hirshfeld surface analyses and the Monte Carlo method was used to investigate the interfacial interactions of these derivatives with iron, copper, and aluminum surfaces. Based on the Monte Carlo results, the new compounds can provide better anti-corrosion properties for iron as compared to copper and aluminum. The expected inhibition efficiency is 5a > 3a > 4a, which is attributed to the favorable effect of the lateral carbon chain of the triazole moiety in this process. The antibacterial activities of 1, 2, 3a, 3b, 4a, 5a, 5b, 6a, and 6b against Gram-positive and Gram-negative microbial strains, such as Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were evaluated, and the results obtained showed antibacterial activities for all of them using a minimum inhibitory concentration (MIC) test. (C) 2021 Elsevier B.V. All rights reserved
Production and Evaluation of an Inactivated Adjuvanted Vaccine against Canine Parvovirus in Morocco
No full textThe study conducted in Morocco focused on addressing the challenges posed by canine parvovirus (CPV-2) through comprehensive research, vaccine development, and efficacy assessment. Through real-time PCR screening and genotyping, CPV-2 variants were identified circulating in the region. An inactivated vaccine, derived from a CPV-2 strain isolated from a symptomatic dog, was produced and evaluated for safety and efficacy. The vaccine, from the strain named “CaPV M/3-2022”, demonstrated safety in vaccinated puppies, with no adverse reactions observed during the trial period. Efficacy trials showed that vaccinated puppies remained healthy and exhibited lower viral excretion post-challenge compared to unvaccinated controls. These results indicate that the vaccine effectively protects against illness related to CPV-2 and reduces viral shedding. The study provides valuable insights into CPV-2 epidemiology in Morocco, offers a promising vaccine solution, and underscores the importance of vaccination in controlling CPV-2 outbreaks and protecting canine health
Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco
No full text
