46 research outputs found
Distributed compression for the uplink channel of a coordinated cellular networkwith a backhaul constraint
No full textEnhanced protein secretion in reduced genome strains of Streptomyces lividans
No full textHamed MB, Busche T, Simoens K, et al. Enhanced protein secretion in reduced genome strains of Streptomyces lividans. Microbial Cell Factories . 2024;23(1): 13.BACKGROUND: S. lividans TK24 is a popular host for the production of small molecules and the secretion of heterologous protein. Within its large genome, twenty-nine non-essential clusters direct the biosynthesis of secondary metabolites. We had previously constructed ten chassis strains, carrying deletions in various combinations of specialized metabolites biosynthetic clusters, such as those of the blue actinorhodin (act), the calcium-dependent antibiotic (cda), the undecylprodigiosin (red), the coelimycin A (cpk) and the melanin (mel) clusters, as well as the genes hrdD, encoding a non-essential sigma factor, and matAB, a locus affecting mycelial aggregation. Genome reduction was aimed at reducing carbon flow toward specialized metabolite biosynthesis to optimize the production of secreted heterologous protein.; RESULTS: Two of these S. lividans TK24 derived chassis strains showed~15% reduction in biomass yield, 2-fold increase of their total native secretome mass yield and enhanced abundance of several secreted proteins compared to the parental strain. RNAseq and proteomic analysis of the secretome suggested that genome reduction led to cell wall and oxidative stresses and was accompanied by the up-regulation of secretory chaperones and of secDF, a Sec-pathway component. Interestingly, the amount of the secreted heterologous proteins mRFP and mTNFalpha, by one of these strains, was 12 and 70% higher, respectively, than that secreted by the parental strain.; CONCLUSION: The current study described a strategy to construct chassis strains with enhanced secretory abilities and proposed a model linking the deletion of specialized metabolite biosynthetic clusters to improved production of secreted heterologous proteins. © 2023. The Author(s)
Compress-And-Forward Cooperative Relaying In Mimo-Ofdm Systems
No full textIn this paper, we investigate the capacity of Compressand -Forward (C&F) cooperative relaying scheme when the C&F relay operates in Time Division Duplex (TDD). In our evaluation we consider MIMO-OFDM transmission. An achievable rate was previously derived in [6] assuming scalar channel. We extend this Wyner-Ziv bound to MIMO-OFDM, by applying results from Bayesian vector estimation and rate-distortion coding theory. Then we derive the mutual information of a sub-optimum relaying scheme in which the relay applies Karhunen-Loeve transform to the signal received from the source before quantizing it and forwarding it to the destination as a new codeword. Finally, we illustrate by simulations (in an environment similar to IEEE802.16) the fact that for some scenarios, the C&F approach outperforms other known relaying techniques. This remains true even if the C&F sub-optimum scheme is considered
Use of economic evaluation in decision making: evidence and recommendations for improvement
No full textInformation about the value for money of a medicine as derived from an economic evaluation can be used for decision-making purposes by policy makers, healthcare payers, healthcare professionals and pharmaceutical companies. This article illustrates the use of economic evaluation by decision makers and formulates a number of recommendations to enhance the use of such evaluations for decision-making purposes. Over the last decades, there has been a substantial increase in the number of economic evaluations assessing the value for money of medicines. Economic evaluation is used by policy makers and healthcare payers to inform medicine pricing/reimbursement decisions in more and more countries. It is a suitable tool to evaluate medicines and to present information about their value for money to decision makers in a familiar format. In order to fully exploit the use of economic evaluation for decision-making purposes, researchers need to take care to conduct such economic evaluations according to methodologically sound principles. Additionally, researchers need to take into account the decision-making context. They need to identify the various objectives that decision makers pursue and discuss how decision makers can use study findings to attain these objectives. These issues require further attention from researchers, policy makers, healthcare payers, healthcare professionals and pharmaceutical companies with a view to optimizing the use of economic evaluation in decision making.sponsorship: Financial support for this research project was received from Pharma.be, the Belgian association of the innovative pharmaceutical industry. The sponsor was not involved in the study design; the collection, analysis and interpretation of data; the writing of the manuscript; or the decision to submit the paper for publication. The author has no conflicts of interest that are relevant to the content of this manuscript. (Pharma.be, the Belgian association of the innovative pharmaceutical industry)status: Publishe
Utilisation des réseaux sociaux pour la conception urbaine et architecturale
No full textpeer reviewedthe article focuses on the analysis of new digital media, in particular the socionumeric platforms such as Facebook, Twitter..., writing on these platforms and their impact on architectural or urban projects. In order to deconstruct these new processes of information and data made easily available to the authors of projects, we work from theories allowing to give value (meaning) to writing according to the context in which it is written and materializing in the form of powerful citizen or individual commitments. We are looking for the keys of analysis allowing to pass from an opinion of influence to an analysis of recurrent commitments to be the object of collective stakes. These different approaches of reading the media/medias crossed with the territory and its collective commitments allow us to develop a method of analysis of the stakes involved in the advent of a new project on the scale of a district or a piece of city. Its ambition is to offer any project author (rarely a data scientist) to offer any project author an appropriate approach that is more within his or her reach while being in phase with the digital reality that transcends the traditional approach to project design.11. Sustainable cities and communities17. Partnerships for the goal
Health Technology Assessment in Health Economics
No full textThis chapter introduces health technology assessment and health economics as tools for decision makers to allocate scarce resources in the healthcare sector. It argues that information about the safety, efficacy and effectiveness, organizational implications, social and ethical consequences, legal considerations, and health economic aspects of the application of a health technology needs to be taken into account with a view to informing decisions about the registration and reimbursement of a health technology. Also, the author hopes that understanding the methodology and use of health technology assessment and health economics will persuade the reader of the added value of such studies and promote the application of health technologies that support further health improvements, whilst containing health expenditure.</jats:p
Health Technology Assessment in Health Economics
No full textThis chapter introduces health technology assessment and health economics as tools for decision makers to allocate scarce resources in the healthcare sector. It argues that information about the safety, efficacy and effectiveness, organizational implications, social and ethical consequences, legal considerations, and health economic aspects of the application of a health technology needs to be taken into account with a view to informing decisions about the registration and reimbursement of a health technology. Also, the author hopes that understanding the methodology and use of health technology assessment and health economics will persuade the reader of the added value of such studies and promote the application of health technologies that support further health improvements, whilst containing health expenditure.</jats:p
The use of social networks as a tool for architectural and urban design
No full text[EN] The paper focuses on the analysis of new digital media, in particular the sociometric platforms such as Facebook, Twitter..., the writing on these platforms and their impact on architectural or urban projects. In order to deconstruct these new processes of information and data made easily available to the authors of projects, we work from theories allowing to give value (meaning) to writing according to the context in which it is written and materializing in the form of powerful citizen or individual commitments. We look for the keys of analysis allowing to pass from an opinion of influence to an analysis of recurrent commitments to be the object of collective stakes. These different approaches of reading the media/medias crossed with the territory and its collective commitments allow us to develop a method of analysis of the stakes because of the advent of a new project at the scale of a district or a piece of city. Its ambition is to offer to any project author (rarely a data scientist) to appropriate an approach more within his reach while being in phase with the digital reality that transcends the traditional approach of project design.Institut Numédiart, UMONS, FNRSSimoens, P. (2023). The use of social networks as a tool for architectural and urban design. Editorial Universitat Politècnica de València. 184-197. https://doi.org/10.4995/VIBRArch2022.2022.15134OCS18419
