73 research outputs found
Immunocytochemical staining of Drosophila larval body-wall muscles
No full textFirst author Preethi Ramachandran is a doctoral student in the Neuroscience Program in the Morningside Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.Over the last two decades, the Drosophila larval neuromuscular junction (NMJ) has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. This article describes a procedure for labeling various proteins with antibodies in dissected larval body-wall muscles and visualizing their localization and distribution in the brain, NMJ, and muscle.Neuroscienc
Dissection of Drosophila larval body-wall muscles
No full textFirst author Preethi Ramachandran is a doctoral student in the Neuroscience Program in the Morningside Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.Over the last two decades, the Drosophila larval neuromuscular junction has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. A number of different techniques have been used to dissect third-instar larval preparations to expose the body-wall muscles. Here, we describe a procedure that uses magnetic chambers and pins to allow for fine control in spreading the larval body wall.Neuroscienc
Representation of Women Characters in Preethi Shenoy’s ‘It’s All in the Planets’
No full text \u27It’s all in the Planets\u27 is a charismatic love story written by Preethi Shenoy. It was published in September 2016. The author of the novel, Preethi Shenoy is an Indian author, Speaker and famous blogger. In this paper, I am analysing this novel from the perspective of feminism, especially how the author presented her female characters. Feminism originated as an offshoot of the women\u27s suffrage movement. The major feminist theorist is Toril Moi, Simone de Beauvoir, Elaine Showalter etc. It is a movement against men’s ideologies and society’s certain representations. Their main concern was the representations of women in literature. Traditionally females are represented in literature as passive characters. Though the feminists broke all such representations, our literary works do not completely get rid of such representations. Many of the authors still present women as passive in their works. In this novel too there are such representations. Superficially it is a love story between two persons who are living in two different worlds. They have their tastes, opinions and attitudes. Even they have two life partners (living relationships). But ultimately fate joins them
Improved Low Voltage Ride through Capability of a Fixed Speed Wind Generator Using Dynamic Voltage Restorer
No full textAbstractWind energy is one of the fastest growing renewable energy technologies in world. The new grid code proposes that the wind turbine should remain connected to the grid during voltage disturbances. Three phase voltage sags and faults cause reduction in voltage at the point of interconnection to the grid when fixed speed wind turbines connected to squirrel cage induction generators are employed resulting in disconnection of wind turbine from the grid. Dynamic voltage restorer is a series connected custom power device used for voltage compensation during sags and swells. In this paper, the Low voltage ride through capability of a fixed speed wind turbine is improved using dynamic voltage restorer. Simulation studies are done to determine the transient stability of a fixed speed wind turbine with squirrel cage induction generator using Matlab Simulink
Physiological roles and regulation of the cryptic prophage-encoded small protein DicB and small RNA DicF in Escherichia coli
No full textBacterial adaptation to stress conditions is vital for their survival in different environments. In the past decade, the important roles that small RNAs (sRNAs) and small proteins perform in regulating the stress response in bacteria has become evident. While the roles of numerous sRNAs and small proteins encoded on the bacterial core genome have been characterized, very few that are phage or prophage-encoded have been studied. This dissertation describes the roles and regulation of the sRNA DicF and small protein DicB encoded on the Qin cryptic prophage of Escherichia coli K12. Bacterial genomes harbor cryptic prophages that have lost genes required for induction, excision from host chromosomes, or production of phage progeny. DicB and DicF are encoded on the dicBF operon, which is located in the immunity region of the cryptic prophage Qin. DicB and DicF have previously been implicated in inhibiting cell division of the host bacterium. In this study, we show that the small protein DicB protects the host cells from phage infection. DicB specifically inhibits infection by λ and other phages that use ManYZ inner membrane proteins to inject their DNA into the host cell. DicB also inhibits the canonical function of ManYZ, which is mannose sugar transport. We demonstrated that the previously known interaction between DicB and MinC, a host protein involved in proper positioning of the Z ring during cell division, is necessary for the DicB-dependent phenotypes involving ManYZ identified in this study. The sRNA DicF is widely conserved is many E. coli strains and inhibits ftsZ mRNA translation in E. coli. In this study, we establish the mechanism of this regulation by characterizing the base pairing interaction between DicF and ftsZ, and delineate the roles of other host factors involved in this regulation. Additionally, we identify new mRNA targets of the sRNA DicF, that are primarily involved in host cell metabolism. The final part of this thesis work describes the genetic mechanisms defining the regulation of the dicBF operon. The dicBF operon is constantly repressed under laboratory conditions by the repressor DicA. In this work, we identified that the protein Rem impairs DicA repression of the dicBF operon and is the antirepressor of DicA. We show that Rem induces expression of dicB and dicF, which leads to the concomitant cell filamentation phenotype as DicB and DicF are cell division inhibitors. In the absence of the dicBF gene products, the promoter of the dicBF operon was found to undergo spontaneous induction in a subset of cells, reminiscent of the λ phage bistable genetic switch. Lastly, urea and high temperature were identified as strong inducers of the dicBF promoter in strains deleted for the dicBF operon. Our results suggest that expression of the dicBF operon is regulated in multiple ways in E. coli K12, indicative of the complex relationship that exists between the host cell and cryptic prophages.Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-05-01The student, Preethi Narayani Thattai Ragunathan, accepted the attached license on 2020-12-22 at 15:01.The student, Preethi Narayani Thattai Ragunathan, submitted this Dissertation for approval on 2020-12-22 at 16:38.This Dissertation was approved for publication on 2020-12-23 at 13:10.DSpace SAF Submission Ingestion Package generated from Vireo submission #16136 on 2021-09-16 at 17:01:29Made available in DSpace on 2021-09-17T02:34:10Z (GMT). No. of bitstreams: 2
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Previous issue date: 2020-12-23Embargo set by: Seth Robbins for item 118462
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Stability Analysis of a Discrete Time Model on Plant-Soil Interactions
No full textIn this paper, a nonlinear mathematical model on plant soil interactions is proposed and analyzed. We consider the variables namely, density of the plant species, nutrient concentration in the soil and in the plant for metabolic activity. We consider that the growth rate of the plant species is dependent on the density of the nutrient concentration in the plant. The relationship between the concentration and the rate of uptake is often described quantitatively by Michaelis?Menten kinetics. We discretize the model by applying Backward Euler method and analyse the stability of the model both locally and globally. We analyse the nutrient concentration in Tomato plant and provide numerical simulations for the dynamical behaviour of plant soil interactions for each nutrient. The numerical simulations are provided using MATLAB
A Mathematical Model on Deforestation Due to Human Population and Its Effect on Farm Fields: Role of Technology in Its Conservation
No full textP97. Oral cancer profile in Trivandrum – Capital City of Kerala, P.G. Balagopal, Nebu Abraham George, Preethi Sara George, Aleyamma Mathew, Paul Sebastian, Regional Cancer Centre, Trivandrum, Kerala, India
No full textA Game Theory Approach for Efficient Node Clustering Based on Unstable Ranging Transmission in MANET
No full textIn Mobile Ad Hoc Network (MANET), improving scalability, energy efficiency and network lifetime are the most important and challenging issues. Node Clustering is a well known solution for handling these issues. While existing work focused on stable transmission range, this paper uses unstable ranges transmission for clustering nodes in network. Game theory has been used in fields of science such as economics and biology, but recently it was used in MANET for clustering problem. Grouping mobile nodes into convenient clusters can reduce routing overhead and provide more scalable solution. The main objective of this work is to clustering of nodes in MANET and selects energy efficient cluster head to improve the network lifetime using game theoretical model. This paper proposes an efficient algorithm for Node Clustering based on Unstable Ranging Transmission (NCURT). In addition to this, a game theory based cluster head (CH) selection algorithm is proposed to selects CH based on Evolutionary game theory approach where each node behaves selfish or unselfish based on the strategy selection. Analytical and simulation studies are carried out to assess the performance of the proposed cluster formation and cluster head selection algorithm. The numerical results verify that the proposed approach is effective and efficient to guarantee life time and energy efficiency in MANET. </jats:p
Isolation, Purification and Partial Characterization of DDMS- Dehydrochlorinase from Pseudomonas Dimunita
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
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