2,845 research outputs found
Supplemental Material, SPPS699252_suppl_mat - A Longitudinal Test of Three Theories of Overconfidence
No full textSupplemental Material, SPPS699252_suppl_mat for A Longitudinal Test of Three Theories of Overconfidence by Sean C. Murphy, Fiona Kate Barlow, and William von Hippel in Social Psychological and Personality Science</p
Collaboration and interconnectivity: Nottinghamshire Fire and Rescue Services and higher education institutions in Nottingham
No full textThis paper will describe the developing relationship between Nottinghamshire Fire and Rescue Services and the two Higher Education Institutions in Nottingham. It will chronicle how a very traditional relationship has been transformed, initially by a simple consultancy project, into a much closer working relationship characterised by a much richer variety of collaborative projects. It demonstrates the potential mutual benefits that greater trust and reciprocity between the institutions can bring to both academia and to practice and the impact it has already had on curriculum development, teaching and learning in Nottingham
Lee_Supplemental_Material_rev – Supplemental material for Sex Differences in Misperceptions of Sexual Interest Can Be Explained by Sociosexual Orientation and Men Projecting Their Own Interest Onto Women
No full textSupplemental material, Lee_Supplemental_Material_rev for Sex Differences in Misperceptions of Sexual Interest Can Be Explained by Sociosexual Orientation and Men Projecting Their Own Interest Onto Women by Anthony J. Lee, Morgan J. Sidari, Sean C. Murphy, James M. Sherlock and Brendan P. Zietsch in Psychological Science</p
Lee_OpenPracticesDisclosure_rev – Supplemental material for Sex Differences in Misperceptions of Sexual Interest Can Be Explained by Sociosexual Orientation and Men Projecting Their Own Interest Onto Women
No full textSupplemental material, Lee_OpenPracticesDisclosure_rev for Sex Differences in Misperceptions of Sexual Interest Can Be Explained by Sociosexual Orientation and Men Projecting Their Own Interest Onto Women by Anthony J. Lee, Morgan J. Sidari, Sean C. Murphy, James M. Sherlock and Brendan P. Zietsch in Psychological Science</p
Supplementary_Materials_for_SPPS_Manuscript_Final - Preferences for Sexually Dimorphic Body Characteristics Revealed in a Large Sample of Speed Daters
No full textSupplementary_Materials_for_SPPS_Manuscript_Final for Preferences for Sexually Dimorphic Body Characteristics Revealed in a Large Sample of Speed Daters by Morgan J. Sidari, Anthony J. Lee, Sean C. Murphy, James M. Sherlock, Barnaby J. W. Dixson and Brendan P. Zietsch in Social Psychological and Personality Science</p
The road to progressive taxation
No full textRichard Murphy examines the recent increase in the rate of tax for high income earners in the UK and argues that this should be the beginning of a journey whose destination is a wholly progressive taxation system. Copyright (c) 2009 The Author. Journal compilation (c) 2009 ippr.
The Thermal Decomposition of 1,2-Dioxetane Revisited
No full textThe ground state (S0) and lowest energy triplet state (T1) energy surfaces of the parent dioxetane have been extensively explored using various CASSCF active spaces with MP2 corrections in several basis sets. In particular, the singlet/triplet surface crossing regions have been examined and the spin - orbit coupling and energetics computed. The computed energy barrier for the ring-opening of dioxetane is 16 kcal mol-1, which is lower than the experimentally observed threshold (22 kcal mol-1) for unsubstituted dioxetane decomposition. However, the surface topology is in good agreement with the experimental observations. The barrier for O - O cleavage on the ground state surface is found to lie at nearly the same energy as the transition structure for C - C biradical cleavage on the triplet energy surface. More significantly, the computational results indicate that the singlet and triplet surfaces do not cross along the minimum energy path (MEP) between the ground state O - O cleavage transition state and the singlet biradical. as previously thought. Instead, the S0 → 3(3π) surface crossing is prompted by a motion orthogonal to the reaction coordinate, which has components along both the OC - CO torsional and O - C - C asymmetric bending vibrational modes. In particular, we find evidence for a singlet/triplet crossing "line" that spans the ground state O - O cleavage valley and lies a few kcal mol-1 higher in energy. The computed spin - orbit coupling between the ground state and triplet 3(3π) surfaces is large (ca. 60 cm-1) throughout this crossing region. Therefore it is suggested that facile intersystem crossing (ISC) from the ground state to the triplet surface can occur anywhere along the MEP. ISC leads to production of a .OCH2 - CH2O. triplet biradical that can either fragment to form triplet products or undergo ISC back to the ground state surface. The existence of a triplet/singlet crossing region located very close to the computed triplet biradical, suggests that this species is metastable with a short (picosecond) lifetime
sj-doc-1-tai-10.1177_20499361221108005 – Supplemental material for Design and protocol of the Buprenorphine plus Outpatient Parenteral Antimicrobial Therapy (B-OPAT) study: a randomized clinical trial of integrated outpatient treatment of opioid use disorder and severe, injection-related infections
No full textSupplemental material, sj-doc-1-tai-10.1177_20499361221108005 for Design and protocol of the Buprenorphine plus Outpatient Parenteral Antimicrobial Therapy (B-OPAT) study: a randomized clinical trial of integrated outpatient treatment of opioid use disorder and severe, injection-related infections by Laura C. Fanucchi, Sean M. Murphy, Hilary Surratt, Shashi N. Kapadia, Sharon L. Walsh, James A. Grubbs, Alice C. Thornton, Paul Nuzzo and Michelle R. Lofwall in Therapeutic Advances in Infectious Disease</p
Method validation for dried blood spot Plasmodium 18S rRNA RT-PCR on Roche cobas automated molecular diagnostic platform
No full textThesis (Master's)--University of Washington, 2025Malaria, caused by Plasmodium spp., is a major health burden worldwide. Plasmodium falciparum is responsible for the most severe cases and most human mortality in sub-Saharan Africa. Conventional diagnostic approaches like microscopy and rapid diagnostic tests (RDTs) provide point-of-care screening but lack sufficient sensitivity for low-density infections and surveillance in endemic regions. Molecular diagnostic platforms, particularly nucleic acid tests (NATs), which target highly abundant Plasmodium 18S ribosomal RNA (rRNA), have emerged as sensitive and specific platforms for malaria detection and quantification. The FDA approved Roche cobas® 6800 system, which is a fully automated, high-throughput molecular platform assay for the qualitative detection of Plasmodium spp. from whole blood samples by targeting the 18S rRNA.This study aimed (1) to adapt the cobas® malaria assay for off-label use with non-standard sample types, including 50 μL of whole blood and dried blood spot (DBS) samples, and (2) to generate a standard curve for absolute quantification of the parasites. To achieve this, asexual-stage P. falciparum cultures were established and diluted to generate a full panel of validation samples across clinically relevant densities (1 x 10⁸–1 x 10¹ parasites/mL). Fifty- microliter blood samples were spotted onto DBS cards. Together, whole blood liquid and DBS samples were processed then tested using the cobas® 6800 system. Plasmodium Armored RNA calibrators were used to generate standard curves for quantification. The study evaluated performance metrics parameters, that included pilot feasibility, standard curve assessment, linearity, limit of detection (LoD), precision, accuracy, carryover, DBS RNA stability under different storage conditions, calibrators-sample matrix matched assessment and extraction buffer elution assessment.
The modified FDA-approved Roche cobas® 6800 malaria assay demonstrated successful adaptation for detecting and quantifying P. falciparum 18S rRNA in 50 µL whole blood samples. However, performance was reduced for DBS samples. Both whole blood and DBS sample formats achieved ≥95% detection at the 100 parasites/mL threshold. A matrix mismatch using liquid calibrators to quantify DBS samples resulted in systematic underestimation bias of +0.79 log₁₀ parasites/mL due to poor RNA recovery from DBS samples. The use of DBS-specific calibrators corrected the loss with a reduced bias of -0.07 log₁₀ parasites/mL, which enabled for the reliable quantification of P. falciparum concentration in the DBS samples. Low (100 parasites/mL) density DBS samples stored at room temperature (22–25 °C) and -80°C consistently maintained a 100% positivity hit rate across all the two timepoints (weeks 1 and 6). All MID (2000 parasites/mL) parasite density DBS samples exhibited 100% positivity across both time points and temperature storage conditions. The stability of DBS samples indicated reliable qualitative detection of P. falciparum RNA for DBS samples stored at 22-25°C or -80°C conditions, which supports their use in decentralized sample collection.
Overall, these results suggest that the modified FDA- approved Roche cobas® 6800 malaria assay performs well with 50 μL liquid whole blood samples but improvements are needed to achieve optimal results for 50 μL DBS samples
Mass spectrometry of lipids
No full textMass spectrometry plays a unique and important role in lipid biochemistry, serving as a tool to elucidate lipid structure while offering a method of analysis that relies on ion chemistry, an intrinsic property of lipid substances, rather than physical propertiesIn this comprehensive reference, Robert C. Murphy provides readers with an understanding of the general physical concepts of mass spectrometry and its use in the analysis of specific lipid substances. Opening the text with a description of the fundamentals of mass spectrometry, Dr. Murphy discusses currently used mass analyzers and ancillary techniques, including high-resolution mass spectrometry, selected ion recording, tandem mass spectrometry, and database generation, and details specific examples of their use. He then examines state-of-the-art desorption ionization techniques - including electron ionization, chemical ionization, fast atom bombardment, and electron capture ionization techniques - which can supply vital structural information when coupled with tandem mass spectrometry. Subsequent chapters focus on the ion chemistry of specific lipids, with coverage ranging from fatty acids and their derivatives to glycosphingolipids. DrMurphy presents over 80 complete mass spectra of these lipid substances and more than 120 detailed mechanistic schemes describing the fragmentation pathways observed in these mass spectra. The author also demonstrates the use of mass spectrometry for quantitative assays of the lipids discussed and the application of specific ion measurement as a strategy to enhance sensitivity and specificit
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