162,823 research outputs found
[Report to Chief J. E. Curry, by an unknown author #1]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
[Report to Chief J. E. Curry, by an unknown author #2]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
A Systemic Approach to Understand Smartphone Usage in Singapore
No full textAbstract not availableNam C. Nguyen, Ockie J. H. Bosch, Fung Yen Ong, Jin Shan Seah, Athena Succu, Thanh V. Nguyen, Kwamina E. Banso
Thermotactile thresholds before, during and after exposure to hand-transmitted vibration
No full textWarm and cool thresholds before, during, and after exposure to vibration, were investigated. Twelve healthy male volunteers with a mean age of 26.3 years participated in the study. Subjects were screened to exclude those with prior regular exposure to hand-transmitted vibration, diabetes, vascular or neurological disorders and injuries to the right hand. The experiment was performed in a room with ambient temperature of 23°C. Subjects were acclimatised for 10 minutes before the skin temperatures were measured. During the pre-exposure period, there is no significant difference in thresholds between the control condition and the 125-Hz session. With 125-Hz vibration, warm thresholds increased over the five periods and also over the three periods during application of vibration
Investigating the Mechanisms and Specificities of BphI-BphJ, an Aldolase-Dehydrogenase Complex From Burkholderia xenovorans LB400
No full textChapter 3 - Reprinted (adapted) with permission from Baker, P., Carere, J., and Seah, S. Y. (2011) Probing the Molecular Basis of Substrate Specificity, Stereospecificity, and Catalysis in the Class II Pyruvate Aldolase, BphI, Biochemistry 50: 3559-3569. Copyright (2011) American Chemical Society. Chapter 4 - Reprinted (adapted) with permission from Baker, P., and Seah, S. Y. (2012) Rational design of stereoselectivity in the class II pyruvate aldolase BphI, J Am Chem Soc 134: 507-513. Copyright (2012) American Chemical Society. Chapter 6 - Reprinted (adapted) with permission from Baker, P., Hillis, C., Carere, J., and Seah, S. Y. (2012) Protein-protein interactions and substrate channeling in orthologous and chimeric aldolase-dehydrogenase complexes, Biochemistry 51: 1942-1952. Copyright (2012) American Chemical Society.Microbial degradation of aromatic hydrocarbons is imperative for maintaining the global carbon cycle and removing potentially toxic aromatic xenobiotics. This thesis focuses on the characterization of a pyruvate-specific class II aldolase (BphI) and acetaldehyde dehydrogenase (BphJ), the final two enzymes of the bph meta-cleavage pathway in Burkholderia xenovorans LB400. This pathway is responsible for the degradation of the industrial pollutant polychlorinated biphenyls (PCB) and therefore mechanistic characterization of these enzymes can be applied to improve pollutant degradation. BphI catalyzes the aldol cleavage of 4-hydroxy-2-oxoacids to pyruvate and an aldehyde while BphJ transforms aldehydes to acyl-CoA, using NAD+ and CoASH as cofactors. Size-exclusion chromatography was used to determine that the oligomeric unit of the BphI-BphJ complex is a heterotetramer. The aldolase BphI was shown to exhibit a compulsory order mechanism and utilize 4-hydroxy-2-oxoacids with an S configuration at C4. The generation of BphI active site variants allowed for the proposal of a catalytic mechanism and a greater understanding as to how stereospecificity occurs. Using steady-state kinetic assays, Arg-16 was demonstrated to be essential for catalysis. Molecular modeling of the substrate and pH dependency (wild-type pKa of ~7, lost in H20A and H20S variants) were used to identify His-20 as the catalytic base. Tyr-290 was originally proposed to be the catalytic acid. However, this was refuted as a Tyr-290 (Y290F) variant did not affect the catalytic efficiency of the enzyme. Instead, the variant was observed to exhibit a loss of stereochemical control. From the crystal structure of an orthologous aldolase-dehydrogenase complex, solvent isotope effect studies, and a proton inventory, a water molecule was implicated as the catalytic acid. Based on their position within the crystal structure, Leu-87 and Leu-89 were implicated in substrate specificity. Replacement of Leu-89 with alanine effectively increased the length of the active site, allowing for the accommodation of longer aldehyde substrates. In contrast, Leu-87 was responsible for hydrophobic stabilization of the C4-methyl of the substrate. Double variants L87N;Y290F and L87W;Y290F were constructed to enable the binding of 4(R)-hydroxy-2-oxoacids. Polarimetric analysis confirmed that the double variants were able to synthesize 4-hydroxy-2-oxoacids of up to 8 carbons in lengths, which were of the opposite stereoisomer to those produced by the wild-type enzyme. Cys-131 was identified as the catalytic thiol that forms an acyl-enzyme intermediate in the dehydrogenase, BphJ. This enzyme was shown to exhibit similar specificity constants for acetaldehyde and propionaldehyde and utilize aliphatic aldehydes from two to five carbons in length as substrates. The enzyme was able to use either NAD+ or NADP+ as the cofactor. Finally, we demonstrated that aldehydes produced in the aldolase reaction are not released into the bulk solvent but are channeled directly to the dehydrogenase, providing the first biochemical determination of substrate channeling in any aldolase-dehydrogenase complex.Natural Sciences and Engineering Research Council of CanadaOntario Graduate Scholarship in Science and Technolog
Allium ullengense H. J. Choi & N. Friensen 2019
No full textAllium ullengense H.J. Choi & N. Friensen (2019: 294) Isotype: KOREA, Gyeongsangbuk-do: Ulleng-gun, Buk-myeon, Nari, 5 June 2019, H. J. Choi 190605-001 (NIBRVP781252; Fig. 1 -32). Note: This isotype was donated by KH. The holotype is conserved in KH (KHB1592342!).Published as part of Jang, Hyun-Do, Hyun, Chang-Woo, Ryu, Seah & Lee, Sang-Jun, 2022, Type specimens of vascular plants in the herbarium of the National Institute of Biological Resources (II), pp. 229-243 in Phytotaxa 539 (3) on page 239, DOI: 10.11646/phytotaxa.539.3.2, http://zenodo.org/record/636408
Allium dumebuchum H. J. Choi 2021
No full textAllium dumebuchum H.J. Choi (2021: 8) Isotype: KOREA, Gyeongsangbuk-do: Ulleung-gun, Namyang, 8 October 2020, H. J. Choi 201008-001 (NIBRVP815478; Fig. 1 -31). Paratypes: KOREA, Gyeongsangbuk-do: Ulleung-gun, 3 September 2008, S. T . Kim & S. K. So SK2008-019 - 096 (NIBRVP205843); Ulleung-gun, 15 October 2009, S. H . Kim ksh84 (NIBRVP246241); Ulleung-gun, Gallyeong, 23 August 2005, H . Kim KH1283 (NIBRVP203887), H . Kim & M. J. Song 1073 (3 sheets, NIBRVP182436), H . Kim, M. J. Song & D. H. Shin 1406 (NIBRVP732384); Hyeonpo, 4 October 2011, J. H . Park & K. H. Kim 19-1 (2 sheets, NIBRVP380193); Nari Basin, 25 September 2001, J. S . Kim s.n. (NIBRVP307168); Sadong-ri, 12 October 2005, J. O . Hyun, J. D. Kim & M. A. Jung NAPI-20101161 (2 sheets, NIBRVP294032). Note: The holotype is preserved in KH, and other isotypes are deposited in the herbaria of Changwon National University, KH, and KIOM.Published as part of Jang, Hyun-Do, Hyun, Chang-Woo, Ryu, Seah & Lee, Sang-Jun, 2022, Type specimens of vascular plants in the herbarium of the National Institute of Biological Resources (II), pp. 229-243 in Phytotaxa 539 (3) on page 239, DOI: 10.11646/phytotaxa.539.3.2, http://zenodo.org/record/636408
R. P. De Guzman et M. A. Reforma. Government and Politics of the Philippines J. S. T. Quah, C. H. Chan et C. M. Seah. Government and Politics of Singapour Somsakdi Xuto. Government and politics of Thailand
No full textLechervy. R. P. De Guzman et M. A. Reforma. Government and Politics of the Philippines J. S. T. Quah, C. H. Chan et C. M. Seah. Government and Politics of Singapour Somsakdi Xuto. Government and politics of Thailand. In: Politique étrangère, n°2 - 1989 - 54ᵉannée. p. 355
Murder on the mountain: author talk with Peter J. Wosh
No full textAuthor talk by Peter J. Wosh on May 5th, 2022, on his book, "Murder on the Mountain: crime, passion, and punishment in gilded age New Jersey.
Mr. Melvin J. Collier, RWWL AUC, June 2011
No full textThis video is a conversation with Mr. Melvin J. Collier. Mr. Collier talks about his book, "From Mississippi to Africa: A Journey of Discovery". Daniel Le, AUC Woodruff Library, is the interviewer
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