8,974 research outputs found
Correspondence from Blake Scott to Oscar E. Monnig, April 7. 1976
No full textLetter to Oscar E. Monnig from Blake Scott inviting Monnig to look at what he suspects are pieces of meteorites.April 7, 1976 Mr Oscar Monnig Dear Sir: Received your card. Was glad to hear [hear] from you. I would be good for you to come out any time and see what I believe is pieces of the large meteorite I sold you thank you for writing Blake D. Scott Blake D. Scott Rt 1 Forestburg, Texas 76239 Oscar Monnig 29 Chelsea Drive Fort Worth TX 7613
RT-PCR primer sequences.
No full textFluorescence-based assays provide sensitive and adaptable methods for point of care testing, environmental monitoring, studies of protein abundance and activity, and a wide variety of additional applications. Currently, their utility in remote and low-resource environments is limited by the need for technically complicated or expensive instruments to read out fluorescence signal. Here we describe the Genes in Space Fluorescence Viewer (GiS Viewer), a portable, durable viewer for rapid molecular assay readout that can be used to visualize fluorescence in the red and green ranges. The GiS Viewer can be used to visualize any assay run in standard PCR tubes and contains a heating element. Results are visible by eye or can be imaged with a smartphone or tablet for downstream quantification. We demonstrate the capabilities of the GiS Viewer using two case studies–detection of SARS-CoV-2 RNA using RT-LAMP and quantification of drug-induced changes in gene expression via qRT-PCR on Earth and aboard the International Space Station. We show that the GiS Viewer provides a reliable method to visualize fluorescence in space without the need to return samples to Earth and can further be used to assess the results of RT-LAMP and qRT-PCR assays on Earth.</div
Accreditation of Prior Learning in Pre-registration Nursing Programmes 2: The Influence of Prior Qualifications on Perceived Learning During the Foundation Year
No full texthe perceive learning of pre-registration students nurses during the foundation programme in relation to their prior entry qualifications is investigated at two universities using a quantitative questionnaire. Having ‘A’ levels or National Vocational Qualifications was found to have a significant influence on an individual’s perceived learning. The effect of having level 3 vocational qualifications had a slight negative influence, whilst that of ‘A’ levels was stronger. Findings in relation to perceived learning and prior experiential learning obtained from the application of this questionnaire were reported earlier (Scott, 2007). The implications of both the findings from Scott (2007) and the analysis are discussed here. No evidence was found to support the notion that those with relevant prior learning suffer costs through having to undertake learning the foundation year
Moving into housing: experiences of developing specialist occupational therapy posts in local authority housing departments
No full textThe pathways for the delivery of adaptations to the homes of people with disabilities are complex, incorporating social care expertise together with funding and building experience from housing professionals. Integrating the housing and social care departments does not necessarily lead to smoother working. A number of authorities have taken the option of moving or seconding occupational therapists from social care into housing to have better coordination of the delivery of adaptations and improvement of working practice between the disparate professionals involved.This qualitative study aimed to explore, through semi-structured interviews, the experiences of nine occupational therapists establishing and developing these new posts. Background information about the establishment of these posts was collated. The thematic analysis of data by two researchers taking an interpretive phenomenological perspective identified five themes: practitioner freedom versus isolation, strategic versus operational roles, professional cultural differences, learning and development, and evaluation and aspiration.For managers founding such posts and for practitioners moving into them, the findings of this study offer insights into what helped and hindered these practitioners, what surprised them and how their development needs were identified, which informed their intentions for the future of their posts
RT-LAMP detection of SARS-CoV-2 RNA.
No full textSamples containing 1000, 500, or 100 copies of SARS-CoV-2 RNA were used as template for RT-LAMP reactions. Reactions were monitored continuously in the GiS Viewer and maximum signal was observed at 30 minutes. Samples 1–2: 1000 copies of RNA, samples 3–4: 500 copies of RNA, samples 5–6: 100 copies RNA, samples 7–8: no template control. Limit of detection for this assay was 100 copies within 15 minutes.</p
Radioactive reverse transcription PCR (RT-PCR) analysis of strain SSB318 complemented with wt or C238/C239
No full text<p><b>Copyright information:</b></p><p>Taken from " and investigation of bacterial type B RNase P interaction with tRNA 3′-CCA"</p><p></p><p>Nucleic Acids Research 2007;35(6):2060-2073.</p><p>Published online 13 Mar 2007</p><p>PMCID:PMC1874595.</p><p>© 2007 The Author(s)</p> PCR products were analyzed on a 10% polyacrylamide/8 M urea gel. Lanes 1–30: total RNA from SSB318 complemented with wt (lanes 1–4 and 13-16), C238 (lanes 5–8, 17–20 and 25–30) or C239 (lanes 9–12 and 21–24) grown at 37°C in the absence of IPTG and in the presence of 2% xylose (w/v); amounts of total RNA were 200 ng in lanes 1–24, 26 and 29, 100 ng in lanes 25 and 28, and 400 ng in lanes 27 and 30. P : presence (+) or absence (−) of a xylose-inducible plasmid-encoded gene. Lanes 1–12 and 25–27: primers specific for ; lanes 13–24 and 28–30: primers specific for the mRNA encoding ribosomal protein S18 (S18). AMV: presence (+) or absence (−) of reverse transcriptase. For details on RT-PCR, see the Material and Methods section. Lanes 25–30 document that the amount of RT-PCR product was sensitive to RNA template concentration. The figure illustrates a representative experiment, but the results shown here were reproduced in five individual experiments using three independent total RNA preparations
RT-PCR analysis of circRPMS1_E4_E3a and circRPMS1_E4_E2 circular RNAs.
No full textA) RNase R resistance of circRPMS1_E4_E3a and circRPMS1_E4_E2. Divergent primers within RPMS1 exons 4 and 3a detect both exon 4 to exon 3a and exon 4 to exon 2 (exon 4 to exon 2 to exon 3a) circular RNAs (validated by sequencing of excised fragments). All PCR reactions were repeated with similar results. B) circRPMS1_E4_E3a and circRPMS1_E4_E2 are detected in stomach cancer biopsies from two patients (Pat 1 and Pat 2). “N” refers to normal adjacent tissue and “T” refers to tumor tissue. All PCR reactions were repeated with similar results. C) RT-qPCR analysis using junction specific primers shows nuclear enrichment for circRPMS1_E4_E3a. Error bars represent standard deviations and were derived from triplicate qPCR reactions. These experiments were repeated once with similar results. Method for calculating fraction cytoplasmic is outlined in the methods section.</p
Prescribing by mental health nurses: the UK perspective
No full textPURPOSE. This article aims to discuss the growth of mental health nurse (MHN) prescribing in the United Kingdom as an exemplar for readers to compare progress in their own countries and context. This study also aims to provide a historical overview of this process in the United Kingdom where MHNs prescribe safely and competently.
CONCLUSIONS. Finally, evidence has shown that MHNs with prescriptive authority are competent when prescribing when compared to psychiatrists.
PRACTICE IMPLICATIONS. Despite organizational barriers and educational concerns, MHN prescribing is becoming embedded in the healthcare context in the United Kingdo
Limits to growth and what keeps a biofilm finite
No full textTwo of the factors, shear erosion and diffusive mass transfer, which limit the growth of heterogeneous biofilms are considered. For permeable beds of particulates, with a regulated throughflow, equating shear induced erosion and biofilm growth, leads to estimates of biofilm thickness and activity which conform with experimental measurements. In the more open environments of pipes and channels, increased thickness of biofilm is not directly balanced by increased cell erosion from the biofilm surface. However increasing thickness leads to growth limitations as diffusion limits the rate of mass transfer to cells deep in the film. For heterogeneous biofilms , consisting of complex clusters intersected by channels, mass transfer into the biofilm is by a combination of advective flow in the channels and diffusive transfer in clusters. In this paper we have considered mass transfer into simplified cluster forms, that is cylinders and hemispheres. Using the concept of critical dimension we have explored some of the implications of these simplified structures. We discuss the limitation to this approach as fluid shear alters the form of these simplified clusters. The viscoelastic properties of the biofilm clusters are being investigated and should allow better prediction of the effect of lateral shear on simple forms. The advection in biofilm channels and the related mass transfer processes needs further investigation
Modular RT-Motion USB Software Framework
No full textPhilips Applied Technologies has developed the RT-Motion USB platform as a compact distributed real-time motion control platform, but the platform can still be improved by developing a more advanced software framework. The goal of this thesis project is to design a modular software framework to complement the RT-Motion USB platform with extendability, flexibility, and configurability. The design focuses on the extendability of the platform by developing foundation building blocks to integrate software extension modules and device drivers easily. The design emphasizes the principle of simplicity to ensure the lowest possible overhead and highest reliability. The firmware is modular, which allows each module to concentrate on its own area. The implementation of the design has been tested and is proved to provide extendability, flexibility, and configurability while incurring low overhead. The improvement to the RT-Motion USB platform is expected to extend the applicability of the RT-Motion USB platform to a broader application range.Microelectronics & Computer EngineeringElectrical Engineering, Mathematics and Computer Scienc
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