2 research outputs found
Mercury concentrations in wild mink (Mustela vison) and river otters (Lontra canadensis) collected from eastern and Atlantic Canada
No full textTotal mercury (Hg) concentrations were measured in the fur, brain and liver of wild mink (Mustela vison) and river otters (Lontra canadensis) collected from eastern and Atlantic Canada. Total Hg concentrations in fur were strongly correlated with levels in the brain and liver. There was no difference in tissue concentrations between male and female mink; however, female otters had significantly higher fur, brain and liver Hg levels than males. Similarly, there was not a significant relationship between Hg concentration and age of mink, whereas in otters, Hg concentrations in all three tissues decreased significantly with age. In both species, only a very small percentage of the variability in Hg concentration was explained by age. After adjusting the data for site-to-site differences in Hg levels, Hg concentrations in the fur of mink infected by the parasite, Dioctophyma renale, were found to be significantly higher than Hg levels in uninfected mink.; Mercury (Hg) concentrations in liver, brain and fur are correlated in mink (Mustela vison) and otters (Lontra canadensis), allowing the use of fur as an indicator of internal tissue concentrations.ID: S0269749108000948; M3: Article; Accession Number: S0269749108000948; Author: Katherine Klenavic (a); Author: Louise Champoux (b); Author: O'Brien Mike (c); Author: Pierre-Y. Daoust (d); Author: R. Douglas Evans (a); Author: Hayla E. Evans (a, ∗); Affiliation: Environmental and Resource Studies, Trent University, 1600 West Bank Drive, Peterborough, ON K9J 7B8, Canada; Affiliation: Service Canadien de la Faune Environnement Canada, 1141 Route de l'Église, c.p. 10100, Sainte-Foy, QC G1V 4H5, Canada; Affiliation: Furbearers and Upland Game, Department of Natural Resources, Kentville, NS B4N 4E5, Canada; Affiliation: Department of Pathology & Microbiology, Atlantic Veterinary College, University of P.E.I., 550 University Avenue, Charlottetown, PEI C1A 4P3, Canada; Keyword: Mercury; Keyword: Mink; Keyword: Otter; Keyword: Age; Keyword: Dioctophyma renale; Number of Pages: 8; Language: English
Investigating the Role of Moringa oleifera in NF-kB Inhibition in T Cells
No full textNuclear Factor of kappa B (NF-κB) is implicated in many diseases and is a target for therapeutic interventions. Many anti-inflammatory pharmacological drugs are derived from plants. Moringa oleifera or the “miracle tree” has health benefits. Moringa extracts have been shown to inhibit inflammatory responses in macrophages through a mechanism involving NF-κB. Moringa also impacts T cell function, but whether it inhibits NF-κB activation in T cells is unknown. Our lab is interested in characterizing the impact of Moringa oleifera on T cells. To specifically investigate the impact of Moringa on NF-κB activation in T cells, we will culture Jurkat E6.1 T cells [5x10 6 cells/ml] in complete RPMI pre-treated with ethanolic Moringa extracts (MOE) [0%, 0.5%, 1%, 2.5%, and 5% w/v] for 1 hour. Cells will be stimulated with anti-CD3 and CD28 [1 mg/ml] for 15, 30, and 60 min. Nuclear and cytosolic extracts will be assayed by Western blotting to assess NF-κB activation. Data from this study will contribute to gaps in knowledge related to how Moringa impacts the function of T cells, key component of the adaptive immune responses; ultimately contributing to the growing knowledge of how Moringa oleifera serves as a promising therapeutic alternative.https://digital.library.ncat.edu/honorscollegesymposium25/1025/thumbnail.jp
