1,720,988 research outputs found
Research on Orobanche crenata Management in Sicily from the 19th to the Early 20th Century
No full textIn order to offer hints for new research, the agronomic techniques used to manage Orobanche crenata in Sicily in the late 19(th) and early 20(th) Century are examined; the publications in which they were originally described can be obtained only with difficulty. Hand pulling, resistant cultivars and late sowing are the techniques used nowadays. Screening for resistance to broomrape of the traditional cultivars of broad bean, and for the influence of sowing density on broomrape infestations, is still to be done
Semiotic Textology as the "Grundlagenwissenschaft" for Philology. János S. Petőfi’s scientific inheritance
No full textThis paper is addressed to philologists and literary critics and it examines the relationship between Literary Criticism/the Science of Literature and Text Linguistics. Natural Text Processing is an interdisciplinary operating module that describes, analyses, explains, and evaluates texts. From a chronological perspective, literary criticism is the last discipline of the module, and text linguistics plays the role of Grundlagenwissenschaft. In the field of text linguistics, J. S. Petőfi, by combining different types of logical systems, has advanced the most complete theory, one which constitutes the convergence between the two areas of research on natural language, analytic philosophy, and phenomenology. Thanks to Semiotic Textology, disambiguated representations of a text are produced that render the textual analyses highly explicit and discussable, and an optimum theoretical framework is established within which philologists can develop a method of analysis that takes all relevant semiotic aspects into consideration
Chamaerops humilis L.: variabilità ed ecologia delle popolazioni naturali in Sicilia. Prima parte.
No full textChamaerops humilis L.: variabilità ed ecologia delle popolazioni naturali in Sicilia. Seconda parte.
No full textApplication of the DNA barcoding in the identification of Mediterranean wild plants with ornamental attitude.
No full textDiscovery of molecular markers for early detection of Rhynchophorus ferrugineus attacks in Phoenix canariensis.
No full textI cinghiali (Sus scrofa L.) come fattore di potenziale decremento della palma nana (Chamaerops humilis L.)
No full textEVALUATION OF THE DNA BARCODING APPROACH IN HYPERICUM SPP. DISCRIMINATION
No full textHypericum, with more than 450 species, is widespread in temperate zones all over the world.
In Italy 30 taxa are known (1), 26 species and 4 subspecies; ten of them are native to Sicily, in addition to H. calycinum which is recoded as naturalized.
Hypericum biochemical compounds (flavanols, flavonoids, cumarins, glicosidys, terpens, tannins, essential oils) are well recognized for many pharmacological activities: antiflogistic, improving blood flow, against traumas, in wounds and burns recovering. The most important activity is ascribed to the hipericyn, a compound especially derived from Hypericum perforatum L., with successfully application in anti-depressive phytotherapy.
The medical relevance and the related commercial interest push for improving the taxonomic identification method to dispose of certain plant material. Methods for fast and accurate identification of plant species are required to support morphological characterization.
In this study the potential of the “DNA Barcoding” molecular method was investigated in discriminating the Italian Hypericum taxa in order to develop an easy authentication assay helpful in solving taxonomic doubts or in commercial trade traceability of whole plants, portions or derived products.
The samples range was mainly recovered from native habitats in Italy, during the flowering period. Some samples were also sourced from certified herbarium collection.
The DNA extraction was carried in three biological replicates, according to CTAB protocol for plant material (2). The DNA bank and also the ex-situ collection are stored at CRA-SFM of Bagheria.
The three plastid regions, rbcL, matK and trnH-psbA, were assessed, according to the CBOL Plant Working Group indications (3). Phylogenetic analysis of each molecular marker were conducted by comparing sequences including those available from international databases (BOLD/NCBI) based on Kimura 2-parameter (4). The preliminary results indicate the effectiveness of this method in discriminating the taxa of Hypericum, suggesting the possibility to build a fast and accurate molecular identification method by barcode
Contribution to the identification of Dianthus rupicola (Caryophyllaceae) subspecies using morphological and molecular approaches
No full textThe systematic relationships between the five different subspecies actually recognized within Dianthus rupicola (subsp. aeolicus, subsp. bocchorianus, subsp. hermaensis, subsp. lopadusanus and subsp. rupicola), were assessed by means of morphometric and molecular analysis. Our results highlighted the molecular differences between the five subspecies of D. rupicola and a morphological differentiation of four groups being D. rupicola subsp. aeolicus and D. rupicola subsp. rupicola partly overlapping. At the present state of knowledge, a subspecific status seems the most appropriate for all the recognized subspecies of D. rupicola. The names D. aeolicus, D. bisignanii var. virescens, D. hermaeensis, D. rupicola are here lectotypified respectively on specimens preserved at PAL, NAP, P, and BOLO, while D. bisignanii s.str. is lectotypified on a Tenore’s image and a epitype (at K) is designated. Concerning the name D. rupicola subsp. bocchorianus the holotype is traced
CARATTERIZZAZIONE DELLE EUFORBIE DELLA FLORA SICILIANA TRAMITE “DNA BARCODING”
No full textIl genere Euphorbia L. in Sicilia comprende 36 taxa (1, 2) di cui 8 endemici e subendemici, 21 a distribuzione mediterranea, 5 europea e 2 ancora più ampia. Si tratta di entità presenti nei più svariati contesti ecologici, aventi in comune una caratteristica tossicità derivante dalla presenza di vari alcaloidi più o meno concentrati nel latice.
Alcune euforbie sono tipiche della macchia mediterranea, altre si rinvengono nell’ambito di formazioni prative e forestali di alta quota, alcune sono casmofite, altre psammofite. In particolare, le entità native in Sicilia sono: E. akenocarpa Guss., E. aleppica L., E. biumbellata Poir., E. bivonae Steud., E. ceratocarpa Ten., E. characias L., E. corallioides L., E. cuneifolia Guss., E. dendroides L., E. exigua L. var. exigua, E. exigua var. pycnophylla K. U. Kramer &Westra, E. exigua var. retusa L., E. falcata L. var. falcata, E. falcata var. acuminata (Lam.) St.-Amans, E. gasparrinii Boiss., E. helioscopia L., E. helioscopioides Losc. & Pard., E. heterophylla L., E. hirsuta L., E. lathyris L., E. linifolia L., E. melapetala Gasparr., E. meuselii Raimondo & Mazzola, E. myrsinites L., E. papillaris (Boiss.) Raffaelli & Ricceri, E. paralias L., E. peploides Gouan, E. peplus L., E. pithyusa subsp. cupanii (Bertol.) A. R. Sm., E. platyphyllos L., E. pterococca Brot., E. rigida M. Bieb., E. segetalis L., E. serrata L., E. sulcata Loisel., E. terracina L.
Negli ultimi anni è stato avviato uno studio finalizzato a delineare il profilo genetico e le relazioni filogenetiche tra le succitate entità. Lo studio che finora ha comportato l'analisi di 108 accessioni afferenti ai 36 taxa in questione si prefigge, altresì, di produrre dati utili per la tracciabilità nell’ambito dei settori che richiedono nozioni di carattere tossicologico e allergenico.
Il riconoscimento morfologico dei taxa è stato integrato con quello molecolare basato sulla tecnica del “DNA barcoding” promossa dal CBOL (Consortium for the Barcode of Life). In accordo con i protocolli di analisi delineati per il Regno Vegetale (3) è stato valutato, in prima istanza, il potere discriminante delle regioni plastidiali codificanti rbcL e matK. È stata valutata anche l’utilità della porzione nucleare intergenica ITS2 che si è dimostrata molto efficace nella caratterizzazione di piante utili (p.es. medicinali) e di singole famiglie (p. es. Palmae) (4; 5). A queste regioni potrà essere associato un ulteriore marcatore, relativo alla regione plastidiale IGS trnH-psA. Tale porzione è, infatti, nota per supportare un maggiore grado di discriminazione anche tra specie vegetali affini (6).
Sulla base della divergenza genetica specifica multi-locus, i risultati ottenuti consentiranno di definire le relazioni filogenetiche relative alle euforbie siciliane e produrranno una base di riferimento attendibile anche sotto gli aspetti applicativi
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